Intergrated Analysis of the Prognostic and Immunological Role of an Oncogenic Protein Four Jointed Box 1(FJX1)

Intergrated Analysis of the Prognostic and Immunological Role of an Oncogenic Protein Four Jointed Box 1(FJX1) | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Intergrated Analysis of the Prognostic and Immunological Role of an Oncogenic Protein Four Jointed Box 1(FJX1) Mei Huang, Yan Meng, Jian Ding, Side Liu, Kangmin Zhuang This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-2324834/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background Four jointed box 1(FJX1) has been reported to be up-regulated in many cancers, and has indicated the important role in oncology and immunity. In order to further explore the biological function of FJX1 and find a new immunotherapy targets for cancers, we performed a pan-cancer analysis of FJX1. Methods We used Genotype-Tissue Expression(GTEx), The Cancer Genome Atlas(TCGA), cBioPorta, Immune Cell Abundance Identifier(ImmuCellAI), The Tumor Immune Estimation Resource version2(TIMER2), IMvigor210CoreBiologies and Genomics For Drug Sensitivity In Cancer(GDSC) to explored the potential carcinogenesis and immunological role of FJX1. The FJX1 expression profiles, prognostic value, mutation status, immune cell infiltration, immune-related genes, immunosuppressive pathway-related genes, immunotherapy effect and the IC50 related to FJX1 were analyzed. Cell proliferation assay, transwell migration assay and wound healing assay were conducted in colon cancer cells. Results The results demonstrated that FJX1 was highly expressed in most cancers and was significantly associated with poor prognosis. FJX1 expression were significantly correlated with CNA, DNA methylation, TMB and MSI in various cancers. Tumor associated macrophages (TAMs) was positively correlated with FJX1 in several tumors. Immune-related genes including TGFB1, IL-10, and immunosuppressive pathway-related genes including TGFB1 and WNT1 were positively related to FJX1 expression in most cancers. Moreover, High FJX1 expression could also reduce the efficacy of immunotherapy and contribute to drug resistance. Knocking down FJX1 could inhibit proliferation and migration in colon cancer cells. Conclusion Our study confirmed that FJX1 is an oncogene, a potential novel prognostic biomarker and a new immunotherapy target in pan-cancer. Pan-Cancer FJX1 prognosis biomarker immunotherapy Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-2324834","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":156197693,"identity":"37359750-e9c2-45be-b480-43952883d63e","order_by":0,"name":"Mei Huang","email":"","orcid":"","institution":"Nanfang Hospital, Southern Medical University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Mei","middleName":"","lastName":"Huang","suffix":""},{"id":156197694,"identity":"b35c8f8d-ec20-496e-81e0-930e7d3bb249","order_by":1,"name":"Yan Meng","email":"","orcid":"","institution":"Nanfang Hospital, Southern Medical University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Yan","middleName":"","lastName":"Meng","suffix":""},{"id":156197695,"identity":"49de6e36-b1c6-4cd1-8405-7ea844f37089","order_by":2,"name":"Jian Ding","email":"","orcid":"","institution":"Nanfang Hospital, Southern Medical University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Jian","middleName":"","lastName":"Ding","suffix":""},{"id":156197696,"identity":"a2587d24-ccdf-4673-87e7-bfb7ed6f90fa","order_by":3,"name":"Side Liu","email":"","orcid":"","institution":"Nanfang Hospital, Southern Medical University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Side","middleName":"","lastName":"Liu","suffix":""},{"id":156197697,"identity":"96a0044d-78a9-4ea3-8ff7-c493349e6bdc","order_by":4,"name":"Kangmin Zhuang","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAuUlEQVRIiWNgGAWjYDACHgaGAwkGNnJs7M0HiNbCeOBDRZoxH8+xBKK1MB+cceZw4jyJHAXidMj3nE44zNvGnN7GkMPA8KNiG2EtjL29G4Ba2HLbGM4eYOw5c5uwFmZ+XpAWntw2xr4EZsY2IrSwQbRIpLMx8xgQp4WHt3cD0PsGCWxsxGqR4Dm7ARjICYZtPGwJB4nyi3xP7uYPCQb/5eXnPz744EcFEVpQwAES1Y+CUTAKRsEowAUAGFA8teba/bwAAAAASUVORK5CYII=","orcid":"","institution":"Nanfang Hospital, Southern Medical University","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Kangmin","middleName":"","lastName":"Zhuang","suffix":""}],"badges":[],"createdAt":"2022-11-29 10:44:26","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-2324834/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-2324834/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":29854844,"identity":"7ccc1f7b-a6ad-4621-87af-2d13d92f1ea1","added_by":"auto","created_at":"2022-12-03 11:39:04","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":844183,"visible":true,"origin":"","legend":"","description":"","filename":"manuscripts.pdf","url":"https://assets-eu.researchsquare.com/files/rs-2324834/v1_covered.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Intergrated Analysis of the Prognostic and Immunological Role of an Oncogenic Protein Four Jointed Box 1(FJX1)","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Pan-Cancer, FJX1, prognosis, biomarker, immunotherapy","lastPublishedDoi":"10.21203/rs.3.rs-2324834/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-2324834/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eBackground\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eFour jointed box 1(FJX1) has been reported to be up-regulated in many \u0026nbsp;cancers, and has indicated the important role in oncology and immunity. In order to further explore the biological function of FJX1 and find a new immunotherapy targets for cancers, we performed a pan-cancer analysis of FJX1.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethods\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWe used Genotype-Tissue Expression(GTEx), The Cancer Genome Atlas(TCGA), cBioPorta, Immune Cell Abundance Identifier(ImmuCellAI), The Tumor Immune Estimation Resource version2(TIMER2), IMvigor210CoreBiologies and Genomics For Drug Sensitivity In Cancer(GDSC) to explored the potential carcinogenesis and immunological role of FJX1. The FJX1 expression profiles, prognostic value, mutation status, immune cell infiltration, immune-related genes, immunosuppressive pathway-related genes, immunotherapy effect and the IC50 related to FJX1 were analyzed. Cell proliferation assay, transwell migration assay and wound healing assay were conducted in colon cancer cells.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe results demonstrated that FJX1 was highly expressed in most cancers and was significantly associated with poor prognosis. FJX1 expression were significantly correlated with CNA, DNA methylation, TMB and MSI in various cancers. Tumor associated macrophages (TAMs) was positively correlated with FJX1 in several tumors. Immune-related genes including TGFB1, IL-10, and immunosuppressive pathway-related genes including TGFB1 and WNT1 were positively related to FJX1 expression in most cancers. Moreover, High FJX1 expression could also reduce the efficacy of immunotherapy and contribute to drug resistance. Knocking down FJX1 could inhibit proliferation and migration in colon cancer cells.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusion\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eOur study confirmed that FJX1 is an oncogene, a potential novel prognostic biomarker and a new immunotherapy target in pan-cancer.\u003c/p\u003e","manuscriptTitle":"Intergrated Analysis of the Prognostic and Immunological Role of an Oncogenic Protein Four Jointed Box 1(FJX1)","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2022-12-03 11:38:46","doi":"10.21203/rs.3.rs-2324834/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"d60ca372-3792-4d9b-b059-7fdd0d66f211","owner":[],"postedDate":"December 3rd, 2022","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2022-12-04T23:14:18+00:00","versionOfRecord":[],"versionCreatedAt":"2022-12-03 11:38:46","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-2324834","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-2324834","identity":"rs-2324834","version":["v1"]},"buildId":"7rjqhiLT3MXkJMwkYKINL","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}