Untersuchung der hormonellen Regulation bei Endometriose: Erkenntnisse aus einem Organoidmodell

The paper investigates hormonal regulation in endometriosis using a 3D organoid model derived from endometrial epithelial cells isolated from healthy endometrium (hEMT), eutopic endometrium from endometriosis patients (euEMT), and ectopic endometrium (ecEMT). Organoids from 27 line biobank were generated and validated for similarity to source tissue via immunofluorescence markers (EpCAM, PAX8) and evidence of polarity/differentiation; RNA expression of ESR1, ESR2, PGR, and CYP19A1 was then measured by qPCR after treatment with estradiol, progesterone, and the WNT inhibitor XAV939. While estradiol promoted organoid growth and the expression of CYP19A1 was detected at RNA level across all groups (including after progesterone stimulation and XAV939 modulation), CYP19A1 was reported as under the detection limit after these manipulations, and a comprehensive assessment of ESR1/ESR2/PGR was deferred to ongoing work. This paper is centrally about endometriosis — it uses ectopic and eutopic endometrial organoids to examine differences in estrogen/progesterone signaling and aromatase regulation relevant to endometriosis.

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