Dormancy-Related Genes Define Immune Microenvironment and Prognostic Stratification in Glioblastoma

Dormancy-Related Genes Define Immune Microenvironment and Prognostic Stratification in Glioblastoma | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 28 August 2025 V1 Latest version Share on Dormancy-Related Genes Define Immune Microenvironment and Prognostic Stratification in Glioblastoma Authors : Xu Lu 0009-0001-9147-5823 , Jiaji Liu , Yutao Wang , Xun Sun , Fangzhen Li , Min Zhou , Tao Yang , and Dongxue Li [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.175635744.47435354/v1 106 views 68 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Glioblastoma (GBM) is the most aggressive malignant tumor of the central nervous system, characterized by poor prognosis and limited treatment options. Tumor dormancy, a reversible state of growth arrest, has been proposed as a critical factor in tumor progression, therapeutic resistance, and recurrence, yet its role in GBM remains unclear. In this study, we integrated transcriptomic data from TCGA, CGGA, and GEO cohorts with spatial transcriptomics and experimental validation to investigate the prognostic and immunological significance of dormancy in GBM. A dormancy scoring system based on the weighted expression of positive and negative regulators was established, effectively stratifying patients by survival outcomes. High dormancy scores were associated with reduced neutrophil and macrophage infiltration, an immunosuppressive microenvironment, and improved overall survival. Thirty-one dormancy-related genes were identified, most acting as negative regulators of proliferation, and were used to construct a risk model that demonstrated robust predictive performance across independent cohorts. Spatial transcriptomic and immunohistochemical analyses confirmed the spatial association between dormancy gene expression and low immune cell density. These findings suggest that targeting dormancy–immune interactions could provide a novel strategy for overcoming therapeutic resistance and improving patient stratification in GBM. Supplementary Material File (dormancy-related gene signatures correlate with immune microenvironment and prognosis in glioblastoma.docx) Download 2.28 MB File (fig.2.tif) Download 28.12 MB Information & Authors Information Version history V1 Version 1 28 August 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Authors Affiliations Xu Lu 0009-0001-9147-5823 The First Affiliated Hospital of USTC View all articles by this author Jiaji Liu The First Affiliated Hospital of USTC View all articles by this author Yutao Wang The First Affiliated Hospital of USTC View all articles by this author Xun Sun The First Affiliated Hospital of USTC View all articles by this author Fangzhen Li The First Affiliated Hospital of USTC View all articles by this author Min Zhou The First Affiliated Hospital of USTC View all articles by this author Tao Yang The First Affiliated Hospital of USTC View all articles by this author Dongxue Li [email protected] The First Affiliated Hospital of USTC View all articles by this author Metrics & Citations Metrics Article Usage 106 views 68 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Xu Lu, Jiaji Liu, Yutao Wang, et al. Dormancy-Related Genes Define Immune Microenvironment and Prognostic Stratification in Glioblastoma. Authorea . 28 August 2025. DOI: https://doi.org/10.22541/au.175635744.47435354/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. Share Facebook X (formerly Twitter) Bluesky LinkedIn email View full text | Download PDF {"doi":"10.22541/au.175635744.47435354/v1","type":"Article"} Now Reading: Share Figures Tables Close figure viewer Back to article Figure title goes here Change zoom level Go to figure location within the article Download figure Toggle share panel Toggle share panel Share Toggle information panel Toggle information panel Go to previous graphic Go to next graphic Go to previous table Go to next table All figures All tables View all material View all material xrefBack.goTo xrefBack.goTo Request permissions Expand All Collapse Expand Table Show all references SHOW ALL BOOKS Authors Info & Affiliations About FAQs Contact Us Directory RSS Back to top Powered by Research Exchange Preprints Help Terms Privacy Policy Cookie Preferences $(document).ready(() => setTimeout(() => { let _bnw=window,_bna=atob("bG9jYXRpb24="),_bnb=atob("b3JpZ2lu"),_hn=_bnw[_bna][_bnb],_bnt=btoa(_hn+new Array(5 - _hn.length % 4).join(" ")); $.get("/resource/lodash?t="+_bnt); },4000)); (function(){function c(){var b=a.contentDocument||a.contentWindow.document;if(b){var d=b.createElement('script');d.innerHTML="window.__CF$cv$params={r:'a02655c55a64e2c5',t:'MTc3OTg5ODcwMQ=='};var a=document.createElement('script');a.src='/cdn-cgi/challenge-platform/scripts/jsd/main.js';document.getElementsByTagName('head')[0].appendChild(a);";b.getElementsByTagName('head')[0].appendChild(d)}}if(document.body){var a=document.createElement('iframe');a.height=1;a.width=1;a.style.position='absolute';a.style.top=0;a.style.left=0;a.style.border='none';a.style.visibility='hidden';document.body.appendChild(a);if('loading'!==document.readyState)c();else if(window.addEventListener)document.addEventListener('DOMContentLoaded',c);else{var e=document.onreadystatechange||function(){};document.onreadystatechange=function(b){e(b);'loading'!==document.readyState&&(document.onreadystatechange=e,c())}}}})();