HPTLC and GC-MS profiling with the Biological activity of Cinnamomum zeylanicum Blume (St. bark) essential oil between Indian and African varieties

HPTLC and GC-MS profiling with the Biological activity of Cinnamomum zeylanicum Blume (St. bark) essential oil between Indian and African varieties | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article HPTLC and GC-MS profiling with the Biological activity of Cinnamomum zeylanicum Blume (St. bark) essential oil between Indian and African varieties Manosi Das, Sudipto Debnath, Shuvam Sar, Vijay Kumar, Ranjit Kumar Dey, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7300195/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 06 Oct, 2025 Read the published version in Naunyn-Schmiedeberg's Archives of Pharmacology → Version 1 posted 20 You are reading this latest preprint version Abstract Cinnamomum zeylanicum Blume, known for its medicinal and culinary value, was analyzed for comparative phytochemical profiling and antioxidant potential between Indian (Shillong, Kolkata) and African (Tanzania) stem bark varieties. Using HPTLC and GC-MS, the essential oils revealed key variations in chemical constituents, notably the exclusive presence of eugenol in the Tanzanian variety and higher cinnamaldehyde dimethyl acetal content. Physicochemical and organoleptic differences reflected geographic influence. Antioxidant studies using DPPH and FRAP assays confirmed superior activity in the Tanzanian sample, with the lowest IC50 (22.05 µg/ml) and highest FRAP value (579 µM). Phytochemical screening confirmed the presence of multiple bioactive compounds in the samples. These results underscore the significance of geographical origin in the quality and efficacy of medicinal plants, supporting the need for standardization protocols. This study provides a robust framework for evaluating regional variations in C. zeylanicum , enhancing its pharmacological validation and ensuring authenticity in herbal formulations. Molecular docking study with Eugenol revealed strong binding affinity of eugenol with protein targets iNOS, PPARδ, and PPARα in diabetes, and with acetylcholinesterase in Alzheimer’s disease. Cinnamomum zeylanicum Stem bark Volatile oil HPTLC GC-MS Antioxidant activity Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 06 Oct, 2025 Read the published version in Naunyn-Schmiedeberg's Archives of Pharmacology → Version 1 posted Editorial decision: Revision requested 23 Aug, 2025 Reviews received at journal 22 Aug, 2025 Reviewers agreed at journal 22 Aug, 2025 Reviewers agreed at journal 21 Aug, 2025 Reviews received at journal 21 Aug, 2025 Reviews received at journal 18 Aug, 2025 Reviewers agreed at journal 18 Aug, 2025 Reviewers agreed at journal 18 Aug, 2025 Reviewers agreed at journal 18 Aug, 2025 Reviewers agreed at journal 18 Aug, 2025 Reviewers agreed at journal 18 Aug, 2025 Reviewers agreed at journal 18 Aug, 2025 Reviewers agreed at journal 17 Aug, 2025 Reviewers agreed at journal 16 Aug, 2025 Reviewers agreed at journal 16 Aug, 2025 Reviewers agreed at journal 16 Aug, 2025 Reviewers invited by journal 16 Aug, 2025 Editor assigned by journal 12 Aug, 2025 Submission checks completed at journal 12 Aug, 2025 First submitted to journal 05 Aug, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7300195","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":504480188,"identity":"e296624b-4ba8-466a-8288-0b8e85e3291c","order_by":0,"name":"Manosi Das","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABAklEQVRIiWNgGAWjYNACAwbGBgbmAwwMbBL87A0MDAceEKeFLQGkRbIHqPNAAhH2ALXwGAC1MIC1MODTott+9uDDHwUMsv39Z759+FBmIcEjdvgh0BY7Od0G7FrMzuQlGwPNN55xI3fzzBnnJCR4pNMMgFqSjc0O4NByIMdMGuiXxIYbvJuZedsk6uylE0BaDiRuw6Xl/BszyR9ALfPPn3nM/LcNZEv6B/xabuSYSQAdlrjhQA4zMyNYSw4BW268MQb7ZeONNGPGHrBfcgoOJBjg8cv5HMOHP/4wyM47f/gxw4+yOpDDNn/4UGEnh0sLFPxHFzDAq3wUjIJRMApGAQEAABGyXlcCAbwpAAAAAElFTkSuQmCC","orcid":"","institution":"Central Ayurveda Research Institute, CCRAS, Ministry of Ayush","correspondingAuthor":true,"prefix":"","firstName":"Manosi","middleName":"","lastName":"Das","suffix":""},{"id":504480189,"identity":"6998c5f8-d92c-4348-8d5c-ef1b1717c5e1","order_by":1,"name":"Sudipto Debnath","email":"","orcid":"","institution":"Central Ayurveda Research Institute, CCRAS, Ministry of Ayush","correspondingAuthor":false,"prefix":"","firstName":"Sudipto","middleName":"","lastName":"Debnath","suffix":""},{"id":504480190,"identity":"ef098649-9a1a-403e-a14b-5801c125dc2a","order_by":2,"name":"Shuvam Sar","email":"","orcid":"","institution":"Jadavpur University","correspondingAuthor":false,"prefix":"","firstName":"Shuvam","middleName":"","lastName":"Sar","suffix":""},{"id":504480191,"identity":"47694f20-074b-41f1-8f6a-af6d737f8b64","order_by":3,"name":"Vijay Kumar","email":"","orcid":"","institution":"Central Ayurveda Research Institute, CCRAS, Ministry of Ayush","correspondingAuthor":false,"prefix":"","firstName":"Vijay","middleName":"","lastName":"Kumar","suffix":""},{"id":504480192,"identity":"4b43dece-e38d-4d27-8dfd-9613e0c898ab","order_by":4,"name":"Ranjit Kumar Dey","email":"","orcid":"","institution":"Central Ayurveda Research Institute, CCRAS, Ministry of Ayush","correspondingAuthor":false,"prefix":"","firstName":"Ranjit","middleName":"Kumar","lastName":"Dey","suffix":""},{"id":504480193,"identity":"9fe0c33d-7c08-4ed1-9f96-aba165d7f38a","order_by":5,"name":"Ajay Kumar Meena","email":"","orcid":"","institution":"Ministry of Ayush","correspondingAuthor":false,"prefix":"","firstName":"Ajay","middleName":"Kumar","lastName":"Meena","suffix":""},{"id":504480194,"identity":"b02529ba-82d9-42ae-b558-4b163e131c22","order_by":6,"name":"Saswati Roy","email":"","orcid":"","institution":"Elven Agri Co. 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