Antimicrobial peptide production with Corynebacterium glutamicum on lignocellulosic side streams

Antimicrobial peptide production with Corynebacterium glutamicum on lignocellulosic side streams | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Antimicrobial peptide production with Corynebacterium glutamicum on lignocellulosic side streams Daniel Waldschitz, Mark-Richard Neudert, Jakob Kitzmueller, Jae Hwi Bong, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4942861/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 18 Dec, 2024 Read the published version in Biotechnology for Biofuels and Bioproducts → Version 1 posted 9 You are reading this latest preprint version Abstract Background: Biorefineries usually focus on the production of low-value commodities, such as bioethanol, platform chemicals or single cell protein.Shifting production to bioactive compounds, such as antimicrobial peptides, could provide an opportunity to increase the economic viability of biorefineries. Results: Recombinant production of the antimicrobial peptide pediocin PA-1 in Corynebacterium glutamicum was transferred from yeast extract based media to minimal media based on lignocellulosic spent sulfite liquor.Induced batch, fed batch, and extended batch process modes were compared for highest pediocin PA-1 production. Conclusion: For pediocin PA-1 production on lignocellulosic residues, extended batch cultivation was identified as the optimal process mode, producing up to $\simeq$ 104 mg/L active pediocin PA-1.Moreover, the production of pediocin PA-1 on this sustainable second generation resource exceeded its state-of-the-art production on yeast extract based media $\simeq$ 1.5-fold. lignocellulosic feedstock spent sulfite liquor antimicrobial peptide recombinant production pediocin PA-1 Corynebacterium glutamicum Full Text Additional Declarations No competing interests reported. Supplementary Files Additionalfile1.docx Cite Share Download PDF Status: Published Journal Publication published 18 Dec, 2024 Read the published version in Biotechnology for Biofuels and Bioproducts → Version 1 posted Editorial decision: Revision requested 19 Sep, 2024 Reviews received at journal 17 Sep, 2024 Reviews received at journal 08 Sep, 2024 Reviewers agreed at journal 02 Sep, 2024 Reviewers agreed at journal 02 Sep, 2024 Reviewers invited by journal 02 Sep, 2024 Editor assigned by journal 21 Aug, 2024 Submission checks completed at journal 21 Aug, 2024 First submitted to journal 20 Aug, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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