Promoter Methylation Levels of Progesterone Receptor-B (PR-B) and Expression of mRNA DNA Methyltransferase-1 (DNMT-1) In Menstrual Blood Patients with Endometriosis

Tita Husnitawati Madjid, M Noviar Andri Saputra, Roni Rowawi
In: Research Square · 2023 · doi:10.21203/rs.3.rs-3301543/v1 · W4386553842
preprint OA: green CC0
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⚙ AI-generated summary by claude@2026-06, 2026-06-08 ⓘ

Endometriosis patients exhibited significantly higher progesterone receptor-B promoter methylation and a positive correlation with DNA methyltransferase-1 mRNA expression compared to controls.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-08 ⓘ

This study compared PR-B promoter DNA methylation and DNMT-1 mRNA expression in menstrual blood collected on days 1–2 of the cycle from 20 women with histopathology-confirmed endometriosis and 20 controls without endometriosis, using methylation-specific high-resolution melting for PR-B and qRT-PCR for DNMT-1 mRNA. PR-B promoter methylation levels were significantly higher in the endometriosis group than in non-endometriosis controls (79.129% ± 4.719 vs 28.716% ± 4.732, p 0.05). Correlation analysis within the endometriosis group showed a positive relationship between PR-B methylation and DNMT-1 mRNA expression (r = 0.526, p < 0.05). The paper notes key caveats including its preprint status and the limited scope of assessing eutopic menstrual blood rather than direct tissue-based functional outcomes. This paper is centrally about endometriosis — it investigates PR-B promoter methylation and DNMT-1 expression in menstrual blood as potential markers of progesterone receptor–related epigenetic dysregulation in endometriosis.

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Abstract

Abstract Purpose: This study aimed to analyze the differences and correlation of progesterone receptor-B (PR-B) promoter methylation and mRNA DNA methyltransferase-1 (DNMT-1) expression of eutopic endometrium via menstrual blood between endometriosis patients and non-endometriosis. Patients and methods: We conducted a study of PR-B promoter methylation and mRNA DNMT-1 expression in menstrual blood obtained on the first and second day of the period from forty women with endometriosis and without. Results: Methylation analysis and mRNA analysis revealed a significant difference in the level of DNA methylation of PR-B promoters in the endometriosis group compared to non-endometriosis (79.129%±4.719 vs 28.716% ± 4.732, p 0.05). Conclusion: Correlation analysis showed a positive correlation between PR-B methylation and mRNA DNMT-1 expression in endometriosis. (r=0.526, p < 0.05).

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endometriosis

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