Elemental Imaging and Correlation Analysis of Human Glioblastoma U87 Cells treated with Cisplatin using μ-PIXE

Elemental Imaging and Correlation Analysis of Human Glioblastoma U87 Cells treated with Cisplatin using μ-PIXE | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Elemental Imaging and Correlation Analysis of Human Glioblastoma U87 Cells treated with Cisplatin using μ-PIXE Henrique Fonteles, Theylor Klippel, Julia Marcolin, Deiverti Bauer, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3221778/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Cisplatin (Pt(NH4)2Cl2) and other platinum-based drugs are widely used medications that treat several different cancers, including, but not limited to, ovarian, testicular, and neck. This study presents an investigation into the correlation between the distribution of cellular platinum concentration and other elements in human glioblastoma U87 cells using the μ-PIXE technique. Cells were treated with cisplatin at concentrations of 10, 20, and 100 μM for 24 hours. The μ-PIXE technique enabled the quantification of the elements in the cells and allowed the generation of two-dimensional elemental maps with micrometric spatial resolution. By measuring the chlorine and platinum signals, we were able to identify single cells in the samples and detect those with higher platinum concentrations respectively. Moreover, we observed that the 100 μM sample had a high iron signal correlating with the platinum signal, which was not present at lower treatment concentrations. This result may indicate the induction of ferroptosis, i.e. a type of iron-dependent cell death completely independent from apoptosis. Ion Beam Analysis Cisplatin μ-PIXE U87 Cells Cancer Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3221778","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":223395630,"identity":"0d2d9740-2183-4919-8f06-6385e6f00cae","order_by":0,"name":"Henrique 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