EXPRESSION LEVELS OF FOXO-1, P27KIP1, MIR-27, MIR-186 AND AKT1/AKT-P PROTEINS IN WOMEN WITH ENDOMETRIAL CANCER AND HYPERPLASIA: IMPLICATIONS FOR THE HUMAN REPRODUCTIVE SYSTEM.

Ghaderi P; Fallah S; Khaledi HR; Tehranian A; Rahmati F; Sheikhhasani S

doi:10.4183/aeb.2023.1

EXPRESSION LEVELS OF FOXO-1, P27KIP1, MIR-27, MIR-186 AND AKT1/AKT-P PROTEINS IN WOMEN WITH ENDOMETRIAL CANCER AND HYPERPLASIA: IMPLICATIONS FOR THE HUMAN REPRODUCTIVE SYSTEM.

Ghaderi P, Fallah S, Khaledi HR, Tehranian A, Rahmati F, Sheikhhasani S

Acta endocrinologica (Bucharest, Romania : 2005) · 2023 · doi:10.4183/aeb.2023.1 · PMID:37601705 · PMC10439323

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This study investigated the expression levels of FOXO-1, P27KIP1, miR-27, miR-186, and AKT1/AKT-P proteins in endometrial tissue samples from women with cancer, hyperplasia, and normal controls. The researchers found significantly decreased FOXO-1 and increased AKT-P protein levels in malignant tissues, alongside elevated miR-27 and miR-186 expressions specifically in the cancer group compared to controls. While P27KIP1 levels did not change significantly, the authors concluded that these molecular markers could potentially serve as tools for the early diagnosis and prognosis of endometrial cancer. This paper is centrally about endometriosis and adenomyosis research only insofar as it examines related endometrial pathologies; however, the primary focus remains strictly on endometrial cancer and hyperplasia rather than endometriosis or adenomyosis.

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Abstract

ObjectivesDespite extensive research on endometrial cancer (EC) and endometrial hyperplasia, there is still a gap in understanding the molecular mechanisms underlying their development and progression. The aim of this study was to investigate the expression levels of FOXO-1, P27Kip1, miR-27, and miR-186, and Akt1, Akt-P proteins in patients with EC and endometrial hyperplasia compared to control subjects.Subjects and methodsSamples of the endometrial tumor (n=30), normal (control) (n=30) and endometrial hyperplastic (n=30) tissue were obtained from patients referring to Arash and Imam Khomani hospitals, Tehran, Iran. Expression levels of genes and microRNAs were evaluated by qRT- PCR. Western blot analysis was applied for protein evaluation. The data were analyzed using t-test, Mann -Whitney U, Pearson correlation coefficient analysis, ANCOVA and ANOVA.ResultsThere was significant decrease in FOXO-1 in EC tissue compared to control tissue (p<0.05). Significant increase was observed in expression of miR-27 in patients with EC (p<0.001) and hyperplasia (p<0.01), whereas miR-186 expression level increased significantly only in patients with EC (p<0.05). P27Kip1 expression level did not significantly change in patients with EC and hyperplasia. There was a significant association between expression levels of miR-27 with FOXO-1 and P27Kip1 in patients with EC. Western blot analysis revealed higher endometrial AKT1-P protein levels in patients with EC and hyperplasia than control subjects (p<0.05).ConclusionsOur findings suggest that FOXO-1, miR-27, miR-186, and Akt1-P/Akt1 protein have the potential to serve as tissue biomarkers for early diagnosis, prognosis, and progression of EC in the human reproductive system.

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- Login - Register - Home/Current Issue - About the journal - Editorial board - Online submission - Instructions for authors - Subscriptions - Foundation Acta Endocrinologica - Archive - Contact Romanian Academy The Publishing House of the Romanian Academy ACTA ENDOCRINOLOGICA (BUC) The International Journal of Romanian Society of Endocrinology / Registered in 1938in Web of Science Master Journal List Acta Endocrinologica(Bucharest) is live in PubMed Central Journal Impact Factor - click here. This Article Services Google Scholar PubMed Acta Endocrinologica (Buc) Ghaderi P, Fallah S, Khaledi HR, Tehranian A, Rahmati F, Sheikhhasani S Expression Levels of FOXO-1, P27KIP1, MIR-27, MIR-186 and AKT1/AKT-P Proteins in Women with Endometrial Cancer and Hyperplasia: Implications for the Human Reproductive System Acta Endo (Buc) 2023, 19 (1): 1-9doi: 10.4183/aeb.2023.1 Objectives. Despite extensive research on endometrial cancer (EC) and endometrial hyperplasia, there is still a gap in understanding the molecular mechanisms underlying their development and progression. The aim of this study was to investigate the expression levels of FOXO-1, P27Kip1, miR-27, and miR-186, and Akt1, Akt-P proteins in patients with EC and endometrial hyperplasia compared to control subjects. Subjects and methods. Samples of the endometrial tumor (n=30), normal (control) (n=30) and endometrial hyperplastic (n=30) tissue were obtained from patients referring to Arash and Imam Khomani hospitals, Tehran, Iran. Expression levels of genes and microRNAs were evaluated by qRT- PCR. Western blot analysis was applied for protein evaluation. The data were analyzed using t-test, Mann -Whitney U, Pearson correlation coefficient analysis, ANCOVA and ANOVA. Results. There was significant decrease in FOXO-1 in EC tissue compared to control tissue (p<0.05). Significant increase was observed in expression of miR-27 in patients with EC (p<0.001) and hyperplasia (p<0.01), whereas miR-186 expression level increased significantly only in patients with EC (p<0.05). P27Kip1 expression level did not significantly change in patients with EC and hyperplasia. There was a significant association between expression levels of miR-27 with FOXO-1 and P27Kip1 in patients with EC. Western blot analysis revealed higher endometrial AKT1-P protein levels in patients with EC and hyperplasia than control subjects (p<0.05). Conclusions. Our findings suggest that FOXO-1, miR-27, miR-186, and Akt1-P/Akt1 protein have the potential to serve as tissue biomarkers for early diagnosis, prognosis, and progression of EC in the human reproductive system. Keywords: endometrial cancer, endometrial hyperplasia, biomarker. Correspondence: Soudabeh Fallah (PhD), Department of Biochemistry Faculty of Medicine, Iran University of Medical Sciences, Tehran, 56155, Iran, E-mail: [email protected]

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