ScRNA-seq reveals the chronic methamphetamine-induced immunosuppression in murine PBMC with involvement of dopamine receptor D1

ScRNA-seq reveals the chronic methamphetamine-induced immunosuppression in murine PBMC with involvement of dopamine receptor D1 | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 14 May 2025 V1 Latest version Share on ScRNA-seq reveals the chronic methamphetamine-induced immunosuppression in murine PBMC with involvement of dopamine receptor D1 Authors : Jing-Na Fan , Jin-Ting Zhou , Xiao-Huan Liu , Cheng Cheng , Chun-xia Yan , Li Xiaoming 0009-0007-3700-8558 [email protected] , and Shengbin Li Authors Info & Affiliations https://doi.org/10.22541/au.174723188.81417403/v1 259 views 63 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Background and Purpose: Methamphetamine (METH) is an artificial synthetic non-catecholamine sympathomimetic central stimulant that has been widely abused in recent years, and the abusers are at increased risk of various infectious diseases owing to immune system disorders. Dopamine D1 receptor (DRD1) is a key receptor in the mechanism research of drug addiction and a crucial molecule bridging the nervous and immune systems. Experimental Approach: To understand the peripheral immunomodulatory effect of METH, and the role of DRD1 in this process, we performed single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs) from METH-treated wild-type or DRD1 knockout mice, and controls. Key Results: We report the first specific evidence of the immunosuppressive effects of chronic METH exposure on PBMCs by subset proportion, cell proliferation, cell interaction, gene expression and functional signalling with partial involvement of DRD1. METH shifted a specific B cell subcluster from Ifi27l2a+B to Hba-a2+B, a CD4T cell subcluster from Ifit1+CD4T to Sepp1+CD4T, and a CD8T cell subcluster from Dapl1+CD8T to Lef1+CD8T. Furthermore, scRNA-seq revealed the possible mechanism of DRD1 involvement in METH regulation of immune responses. Conclusion and Implications: The results from the study are expected to be a diagnostic and therapeutic standard of METH abused, and a new therapeutic strategy based on DRD1. Supplementary Material File (figure 1.pdf) Download 10.17 MB File (figure 6.pdf) Download 22.75 MB File (manuscript_-_meth-drd1-pbmc_zjt20250506.docx) Download 188.02 KB File (meth_drd1_pbmc_ sfigure1-3.pdf) Download 1.30 MB Information & Authors Information Version history V1 Version 1 14 May 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords addiction bioinformatics dopamine intracellular signaling Authors Affiliations Jing-Na Fan Xi'an Jiaotong University View all articles by this author Jin-Ting Zhou Xi'an Jiaotong University View all articles by this author Xiao-Huan Liu Xi'an Jiaotong University View all articles by this author Cheng Cheng Xi'an Jiaotong University View all articles by this author Chun-xia Yan Xi'an Jiaotong University View all articles by this author Li Xiaoming 0009-0007-3700-8558 [email protected] Xi'an Jiaotong University View all articles by this author Shengbin Li Xi'an Jiaotong University View all articles by this author Metrics & Citations Metrics Article Usage 259 views 63 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Jing-Na Fan, Jin-Ting Zhou, Xiao-Huan Liu, et al. ScRNA-seq reveals the chronic methamphetamine-induced immunosuppression in murine PBMC with involvement of dopamine receptor D1. Authorea . 14 May 2025. DOI: https://doi.org/10.22541/au.174723188.81417403/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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