Optimization and Application of Rapid Multigene Pcr Chip Based on Liquid Metal Bath

With the outbreak of COVID-19 around the world, rapid and accurate detection of new coronavirus is the key to stop the transmission of the disease and prevent and control the novel coronavirus, among which polymerase chain reaction (PCR) is the mainstream nucleic acid detection method. Temperature cycling device is the core of PCR amplification micro device. The precision of temperature control and temperature change rate directly affect the efficiency of PCR amplification. This study proposes a new polymerase chain reaction method based on multi-gene rapid PCR chip optimization of liquid metal bath, which realizes precise and rapid temperature rise and fall control. We systematically explored the feasibility of using liquid metals with different melting points in the system, and proposed 47°C bismuth based liquid metal bath as the heat conduction medium of the system to optimize the system.The average heating rate of liquid metal bath is fast, and the temperature control stability is good, which can significantly reduce the hysteretic, and the temperature change curve is more gentle, which can greatly improve the efficiency of PCR amplification. The results of gene amplification using rat DNA as the template and SEC61A as the target also indicate that the system can be successfully used in PCR devices. Based on the experiment, we proved that the polymerase chain reaction method optimized by the liquid metal bath multi-gene rapid PCR chip can further improve the temperature response speed. It has the advantages of accurate data, fast response speed, low price, safety and environmental protection.