Uncovering Genetic and Metabolic Determinants of Gibberella Ear Rot Resistance in Diverse Maize Germplasm

Uncovering Genetic and Metabolic Determinants of Gibberella Ear Rot Resistance in Diverse Maize Germplasm | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 27 May 2025 V1 Latest version Share on Uncovering Genetic and Metabolic Determinants of Gibberella Ear Rot Resistance in Diverse Maize Germplasm Authors : Sarah Lipps 0000-0001-8217-1636 , Zachary Hill , Charlette Hasty , Carrie Butt-Wilmsmeyer , Martin Bohn 0000-0003-2364-6229 , and Tiffany Jamann 0000-0002-7288-0866 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.174838140.02592987/v1 207 views 144 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Fusarium graminearum colonizes the maize ear causing Gibberella ear rot (GER) and producing harmful mycotoxins, such as deoxynivalenol (DON) and zearalenone (ZEA), that pose a risk to human and animal health when consumed. The disease can be managed in part by breeding and planting resistant maize cultivars. Resistance to GER is a quantitative and complex trait. Evaluation of diverse germplasm to identify regions and candidate genes associated with resistance may be useful for crop improvement efforts. Screening for GER is time-consuming and costly. Thus, identifying other traits that may serve as a proxy for GER resistance may accelerate resistance breeding efforts. We hypothesized that grain phenylpropanoid content and kernel composition are genetically and mechanistically related to GER resistance. We screened a diverse set of maize inbred lines for disease severity, DON, ZEA, ferulic acid, p-coumaric acid, and several kernel composition traits. Using a genome-wide association study, we identified multiple markers associated with each phenotype and genomic regions that harbor alleles for both disease and metabolite-related phenotypes. We also identified multiple metabolic pathways associated with general biotic defense and potential detoxification. End-season ferulic acid and p-coumaric acid concentrations are not strong proxies for GER resistance, but secondary metabolites are important components of the maize-F. graminearum system. In summary, we identified candidate genes and mechanisms for GER resistance, uncovered a link between phenylpropanoid content and GER, and showed that lignin mutants are more susceptible to infection by F. graminearum. Supplementary Material File (tpg-2025-05-0120-file001.docx) Download 221.73 KB File (tpg-2025-05-0120-file007.docx) Download 41.75 KB Information & Authors Information Version history V1 Version 1 27 May 2025 Copyright This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License Keywords crop genetics genome wide association studies grain maize plant disease Authors Affiliations Sarah Lipps 0000-0001-8217-1636 University of Illinois at Urbana-Champaign College of Agricultural Consumer and Environmental Sciences View all articles by this author Zachary Hill University of Illinois at Urbana-Champaign College of Agricultural Consumer and Environmental Sciences View all articles by this author Charlette Hasty Southern Illinois University Edwardsville View all articles by this author Carrie Butt-Wilmsmeyer Southern Illinois University Edwardsville View all articles by this author Martin Bohn 0000-0003-2364-6229 University of Illinois at Urbana-Champaign College of Liberal Arts and Sciences View all articles by this author Tiffany Jamann 0000-0002-7288-0866 [email protected] University of Illinois at Urbana-Champaign College of Agricultural Consumer and Environmental Sciences View all articles by this author Metrics & Citations Metrics Article Usage 207 views 144 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Sarah Lipps, Zachary Hill, Charlette Hasty, et al. Uncovering Genetic and Metabolic Determinants of Gibberella Ear Rot Resistance in Diverse Maize Germplasm. Authorea . 27 May 2025. DOI: https://doi.org/10.22541/au.174838140.02592987/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); Cited by Sarah Lipps, Aida Z. Kebede, Martin Bohn, Tiffany Jamann, Resistance to Gibberella ear rot in maize: Insights from near‐isogenic line populations, Crop Science, 65 , 6, (2025). https://doi.org/10.1002/csc2.70187 Crossref Loading... View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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