Effect of water intake and storage time on protein concentration and enzyme AChE activity in erythrocyte and plasma blood samples of healthy individuals

Background Storage time influence concentration levels of blood biomarker. This study aimed to assess the effect of water intake prior sampling and storage time on protein concentration, enzyme AChE activity, inhibitor efficacy and to build an efficient inhibitor calibration curve in healthy individuals. Methods Data analysis was performed on 11 participants. Study utilizes substrate acetylcholine chloride and inhibitors BW284c51 (0.01mM) and GUK-987 (0.1mM) . Calibration curve ranging from 10 -1 to 10 -38 mM was build for inhibitor GUK-987 and GDK-510. Data analysis is carried out with Microsoft Excel 2007 . Data analysis was performed via IBM SPSS Statistical Software v23.0 . Descriptive statistics and parametric statistical tests were assessed for 0, 90, 91 and 92 days of storage in Plasma and Erythrocyte samples. Workflow of building calibration curve and the most efficient inhibition concentration is assessed. Results Water intake and storage time have effect on in vitro protein concentration, activation/inhibition of enzyme AChE activity in Plasma and Erythrocyte samples. However, 100% inhibitor efficacy is maintained for inhibitor GUK-987 in Plasma samples and inhibitor BW284c51 in Erythrocyte samples. The most efficient inhibitor concentration is determined. Conclution Significant changes and variable association have been estimated between protein concentration, activation/inhibition of enzyme AChE activity, as a cause of water intake and storage time. Taking all these factors into account for further research is important for disease prevention and human wellbeing.