Morphology of gracilis muscle and the topographic anatomy of its neurovascular pedicles: A cross sectional study

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Murlimanju" }, { "@type": "Person", "name": "Mamatha Tonse" }, { "@type": "Person", "name": "Mangala M. Pai" } ], "publisher": { "@type": "Organization", "name": "F1000Research", "logo": { "@type": "ImageObject", "url": "https://f1000research.com/img/AMP/F1000Research_image.png", "height": 480, "width": 60 } }, "image": { "@type": "ImageObject", "url": "https://f1000research.com/img/AMP/F1000Research_image.png", "height": 1200, "width": 150 }, "description": " Background The objective of this study was to perform morphometry of the gracilis muscle and understand the topographical basis of the entry of its pedicles. Methods We studied forty-four cadaveric lower extremities fixed in formalin. The length and width of the gracilis were measured at three locations: the origin, midpoint, and musculotendinous junction. The topographic location of the gracilis pedicles was also studied. Results The gracilis muscle’s length was 369.9±34.1 mm and 359.6±29.6 mm over the right and left sides. The width of right gracilis was 25±8.4 mm, 20.4±6.2 mm and 10.6±5.6 mm at the origin, midpoint and at the musculotendinous junction. The same over the left side were 26.7±8.6 mm, 20.6±9.1 mm and 10.4±6.4 mm respectively. The number of gracilis ranged from one to three. The location of first pedicle was 93.6±35.6 mm and 68.9±35.8 mm away from the pubic tubercle on the right and left sides, respectively. The second and third pedicles were entering at a distance of 153.1±38.8 mm and 101.3±20.8 mm, 214.6±86.8 mm and 145.3±124.4 mm over the right side and left side. The accessory head of the origin of the gracilis was observed in only one cadaver (2.3%), which originated from the adductor longus. Conclusions It is believed that the morphological data of the gracilis and its neurovascular pedicles will be enlightening to the operating surgeon. They will guide the procedures for reconstructive plastic surgery. 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F1000Research 2025, 13 :299 ( https://doi.org/10.12688/f1000research.144786.2 ) NOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article. Close Copy Citation Details Export Export Citation Sciwheel EndNote Ref. Manager Bibtex ProCite Sente EXPORT Select a format first Track Share ▬ ✚ Research Article Revised Morphology of gracilis muscle and the topographic anatomy of its neurovascular pedicles: A cross sectional study [version 2; peer review: 2 approved] Chettiar Ganesh Kumar 1 , Rajanigandha Vadgaonkar https://orcid.org/0000-0001-5577-495X 1 , M.D. Prameela 1 , [...] Vandana Blossom 1 , B.V. Murlimanju https://orcid.org/0000-0003-1248-8296 1 , Mamatha Tonse https://orcid.org/0000-0003-1322-6730 1 , Mangala M. Pai https://orcid.org/0000-0001-6995-5807 1 Chettiar Ganesh Kumar 1 , Rajanigandha Vadgaonkar https://orcid.org/0000-0001-5577-495X 1 , [...] M.D. Prameela 1 , Vandana Blossom 1 , B.V. Murlimanju https://orcid.org/0000-0003-1248-8296 1 , Mamatha Tonse https://orcid.org/0000-0003-1322-6730 1 , Mangala M. Pai https://orcid.org/0000-0001-6995-5807 1 PUBLISHED 12 Nov 2025 Author details Author details 1 Department of Anatomy, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, India Chettiar Ganesh Kumar Roles: Data Curation, Writing – Original Draft Preparation Rajanigandha Vadgaonkar Roles: Data Curation, Writing – Review & Editing M.D. Prameela Roles: Data Curation Vandana Blossom Roles: Data Curation B.V. Murlimanju Roles: Conceptualization, Data Curation, Formal Analysis, Investigation, Methodology, Project Administration, Writing – Original Draft Preparation Mamatha Tonse Roles: Data Curation Mangala M. Pai Roles: Writing – Review & Editing OPEN PEER REVIEW DETAILS REVIEWER STATUS This article is included in the Manipal Academy of Higher Education gateway. Abstract Background The objective of this study was to perform morphometry of the gracilis muscle and understand the topographical basis of the entry of its pedicles. Methods We studied forty-four cadaveric lower extremities fixed in formalin. The length and width of the gracilis were measured at three locations: the origin, midpoint, and musculotendinous junction. The topographic location of the gracilis pedicles was also studied. Results The gracilis muscle’s length was 369.9±34.1 mm and 359.6±29.6 mm over the right and left sides. The width of right gracilis was 25±8.4 mm, 20.4±6.2 mm and 10.6±5.6 mm at the origin, midpoint and at the musculotendinous junction. The same over the left side were 26.7±8.6 mm, 20.6±9.1 mm and 10.4±6.4 mm respectively. The number of gracilis ranged from one to three. The location of first pedicle was 93.6±35.6 mm and 68.9±35.8 mm away from the pubic tubercle on the right and left sides, respectively. The second and third pedicles were entering at a distance of 153.1±38.8 mm and 101.3±20.8 mm, 214.6±86.8 mm and 145.3±124.4 mm over the right side and left side. The accessory head of the origin of the gracilis was observed in only one cadaver (2.3%), which originated from the adductor longus. Conclusions It is believed that the morphological data of the gracilis and its neurovascular pedicles will be enlightening to the operating surgeon. They will guide the procedures for reconstructive plastic surgery. READ ALL READ LESS Keywords Gracilis Muscle Flap; Pedicled Flap; Reconstructive Surgical Procedures Corresponding Author(s) B.V. Murlimanju ( [email protected] ) Close Corresponding author: B.V. Murlimanju Competing interests: No competing interests were disclosed. Grant information: The author(s) declared that no grants were involved in supporting this work. Copyright: © 2025 Kumar CG et al . This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. How to cite: Kumar CG, Vadgaonkar R, Prameela MD et al. Morphology of gracilis muscle and the topographic anatomy of its neurovascular pedicles: A cross sectional study [version 2; peer review: 2 approved] . F1000Research 2025, 13 :299 ( https://doi.org/10.12688/f1000research.144786.2 ) First published: 18 Apr 2024, 13 :299 ( https://doi.org/10.12688/f1000research.144786.1 ) Latest published: 12 Nov 2025, 13 :299 ( https://doi.org/10.12688/f1000research.144786.2 ) Revised Amendments from Version 1 The resolution of the images were increased, which has improved the quality of the images. The resolution of the images were increased, which has improved the quality of the images. See the authors' detailed response to the review by Nagavalli Pushpa See the authors' detailed response to the review by Apurba Patra READ REVIEWER RESPONSES Introduction Gracilis is often utilized as a graft in the procedures of reconstruction and functioning muscle transfer. 1 It is excellent for the closure of rectovaginal and rectourethral fistulas. The distal pedicle is used during total knee arthroplasty as a soft tissue graft. 2 , 3 It also has wider application in the management of wounds of the groin, contractures, scars, reanimation of the face, 4 , 5 reconstruction of the mammary gland, 6 and anterior cruciate ligament reconstruction. 7 Additional harvesting of the gracilis has proven to offer better postoperative functional activity, such as in hamstring injury. 8 The main vascular pedicle of the gracilis is from the medial and lateral circumflex femoral arteries, which branch from the deep femoral artery, which enters the gracilis at the junction of its upper and middle third. The distal part of the gracilis is supplied by the femoral artery. 9 After the literature search, we found few studies on the dimensions of the gracilis muscle and topography of the neurovascular pedicle in the Indian population. The goal of this anatomical study was to determine the morphometry of the gracilis and the topography of its pedicles. Methods This study involved formalin-fixed lower-limb specimens that were available at the Department of Anatomy. A total of 44 specimens were studied, of which 25 were right-sided and 19 left-sided. Sex was not considered. Specimens with congenital malformations were excluded from this study. The institutional ethics committee, Kasturba Medical College, Mangalore (Reg. No. ECR/541/Inst/KA/2014/RR-17) has reviewed the protocol of this study and approved on seventeenth of October, 2018 (IEC KMC MLR 10-18/395). After the meticulous dissection of the adductor compartment of the thigh, gracilis muscle was exposed. This was cleaned from its origin to the dissection. The length of the gracilis (AB in Figure 1 ) was measured from its origin to the musculotendinous junction. The width was measured at three different points: the origin (CD in Figure 1 ), midpoint (EF in Figure 1 ), and musculotendinous junction (GH in Figure 1 ). The pedicles of the gracilis were counted, and their distance from the pubic tubercle was measured. The length and width were measured using a measuring tape and digital Vernier caliper (Mitutoyo, Japan), respectively. The topographical location of the pedicle entry was measured using a measuring tape. Each dimension was measured three times by three different persons, and the average was considered to avoid intra-observer bias. SPSS software, https://www.ibm.com/products/spss-statistics (version 27) was used to perform the paired t-test. We confirm that, our institution has obtained a copyright license of this software. The protocol of this study was archived in the dx.doi.org/10.17504/protocols.io.e6nvwdm6dlmk/v1 . Figure 1. Schematic representation of the dimensions measured in this study (AB-length of gracilis, CD-width at origin, EF-width at midpoint, GH-width at musculocutaneous junction). Results The length of right and left gracilis were 369.9±34.1 mm and 359.6±29.6 mm ( Table 1 ). The width of right gracilis was 25±8.4 mm, 20.4±6.2 mm and 10.6±5.6 mm at the origin, midpoint and at the musculotendinous junction. The same over the left side were 26.7±8.6 mm, 20.6±9.1 mm and 10.4±6.4 mm respectively ( Table 1 ). There were no statistically significant differences in this side-based comparison (p>0.05, paired t-test). The number of pedicles ranged from one to three ( Figures 2 and 3 ). The first pedicle was 93.6±35.6 mm and 68.9±35.8 mm away from the pubic tubercle on the right and left side. The second and third pedicles were entering at a distance of 153.1±38.8 mm and 101.3±20.8 mm, 214.6±86.8 mm and 145.3±124.4 mm over the right side and left side ( Table 2 ). The left gracilis had a proximal second pedicle compared to the right side (p<0.05). The accessory head of origin of the gracilis was observed in only one cadaver (2.3%), which originated from the adductor longus ( Figure 4 ). Table 1. Side based comparison of measurements of gracilis muscle (n=44). Dimension Right side (n=25) Left side (n=19) Length 369.9±34.1 359.6±29.6 Width at its origin 25±8.4 26.7±8.6 Width at midpoint 20.4±6.2 20.6±9.1 Width at musculotendinous junction 10.6±5.6 10.4±6.4 * Significance, p>0.05. Figure 2. Right lower extremity of the embalmed cadaver showing the single neurovascular pedicle (indicated by arrow) of the gracilis. Figure 3. Left lower extremity of the embalmed cadaver showing the two neurovascular pedicles (indicated by the arrows) entering the gracilis. Table 2. Topographic distance of entrance of neurovascular pedicles of gracilis from its origin (n=44). Pedicle Right side (n=25) Left side (n=19) First 93.6±35.6 68.9±35.8 Second * 153.1±38.8 101.3±20.8 Third 214.6±86.8 145.3±124.4 * Significance, p>0.05. Figure 4. Cadaveric specimen showing the accessory head of origin of gracilis (2.3%) from the adductor longus muscle. Discussion In the total knee arthroplasty, one of the complications is the poor wound healing and cutaneous necrosis, which requires thin pliable tough flaps. These soft tissue defects pose a challenge for filling and pedicling the medial head of the gastrocnemius flap. However, in cases where the gastrocnemius flap is insufficient, the surgeon can opt for a distally placed gracilis muscle flap. 10 , 11 Although the proximal pedicle of the gracilis is extensively utilized in plastic and reconstructive surgeries, distally placed pedicled gracilis flaps are another alternative. The distal pedicle is simple and requires less surgical time. 12 , 13 In some situations, a reverse gracilis muscle flap could be performed. 14 The accurate calibration of pedicles and best vasculature of gracilis makes it a best candidate for the distally based pedicle flap. 3 Gracilis receives pedicles ranging between one and five and 75% of the limbs have two vascular pedicles. 15 In our study, the pedicles ranged between one and three. It was reported that the first vascular pedicle is placed at a distance of 105±20 mm from the pubic tubercle. 15 In the present study, this distance was 93.6±35.6 mm on the right lower extremity and left lower extremity had still proximal topography of the first pedicle. In another study, 16 it was reported that the proximal and distal pedicles of the gracilis were located 60 mm and 266 mm away from the pubic tubercle. We observed the distal pedicle at 214.6±86.8 mm and 145.3±124.4 mm away over the right and left sides. In another study, the most proximal pedicle entered the gracilis at a distance of 160-180 mm. Topographic data of vascular pedicles have implications in the field of plastic and reconstructive surgeries. In this concept, this study offers a detailed topographic anatomy of the pedicles in the gracilis muscle. This study also provided the length and width of the gracilis; however, thickness was not measured. This can be considered as a limitation of the present study. Age- and sex-based segregation were not possible. This study had a smaller sample size, and the data were better interpreted based on collections from a larger sample size. The observed length of gracilis in this study was 369.9±34.1 mm and 359.6±29.6 mm over the right and left sides. This is smaller than previously published data, which reported it to be 410±21 mm, 1 the difference is due to ancestral variations. Caucasians were tall and robust, and our samples had smaller dimensions. In another study, the length of gracilis was 432±20.8 mm in males and 371±7.6 mm in females. In their study, the distance of pedicle was 94±7.2 mm in males and 79±2.6 mm in females. 17 We could not segregate the specimens into males and females in this study and this assessment was not possible. Conclusion The present study provided morphometric data 18 of the gracilis and the topographical anatomy of the vascular pedicles. These data will assist surgeons in plastic and reconstructive surgery. Ethical consideration The institutional ethics committee, Kasturba Medical College, Mangalore (Reg. No. ECR/541/Inst/KA/2014/RR-17) has reviewed the protocol of this study and approved on seventeenth of October, 2018 (IEC KMC MLR 10-18/395). Data availability Figshare: Medline database search strategy for ‘Morphometry of gracilis’ Figshare: Medline database search strategy for ‘Morphometry of gracilis. figshare. Dataset, https://doi.org/10.6084/m9.figshare.24503989.v1 ’. 18 The project contains the following underlying data (file name – Morphometry of … Gracilis). Data are available under the terms of the Creative Commons Zero “No rights reserved” data waiver (CC0 1.0 Public domain dedication). References 1. Macchi V, Vigato E, Porzionato A, et al. : The gracilis muscle and its use in clinical reconstruction: an anatomical, embryological, and radiological study. Clin. Anat. 2008; 21 (7): 696–704. PubMed Abstract | Publisher Full Text 2. Ulrich D, Roos J, Jakse G, et al. : Gracilis muscle interposition for the treatment of recto-urethral and rectovaginal fistulas: a retrospective analysis of 35 cases. J. Plast. Reconstr. Aesthet. Surg. 2009; 62 (3): 352–356. PubMed Abstract | Publisher Full Text 3. Tiengo C, Macchi V, Vigato E, et al. : Reversed gracilis pedicle flap for coverage of a total knee prosthesis. J. Bone Joint Surg. Am. 2010; 92 (7): 1640–1646. PubMed Abstract | Publisher Full Text 4. Hussey AJ, Laing AJ, Regan PJ: An anatomical study of the gracilis muscle and its application in groin wounds. Ann. Plast. Surg. 2007; 59 (4): 404–409. PubMed Abstract | Publisher Full Text 5. Hassan KM, El-Moghazy AEAM, Mahmoud MA, et al. : Study of neurovascular anatomy of the split gracilis muscle for the purpose of facial reanimation. Egypt. J. Plast. Reconstr. Surg. 2009; 33 (2): 261–269. 6. Peek A, Müller M, Ackermann G, et al. : The free gracilis perforator flap: anatomical study and clinical refinements of a new perforator flap. Plast. Reconstr. Surg. 2009; 123 (2): 578–588. PubMed Abstract | Publisher Full Text 7. Candal-Couto JJ, Deehan DJ: The accessory bands of gracilis and semitendinosus: an anatomical study. Knee. 2003; 10 (4): 325–328. PubMed Abstract | Publisher Full Text 8. Yosmaoglu HB, Baltaci G, Ozer H, et al. : Effects of additional gracilis tendon harvest on muscle torque, motor coordination, and knee laxity in ACL reconstruction. Knee Surg. Sports Traumatol. Arthrosc. 2011; 19 (8): 1287–1292. PubMed Abstract | Publisher Full Text 9. Stranding S, Borley NR, Gray H: Gray’s anatomy: the anatomical basis of clinical practice. 41st ed.Edinburgh: Churchill Livingstone/Elsevier; 2016. 10. Mitsala G, Varey AH, O’Neill JK, et al. : The distally pedicled gracilis flap for salvage of complex knee wounds. Injury. 2014; 45 (11): 1776–1781. PubMed Abstract | Publisher Full Text 11. Tetreault MW, Della Valle CJ, Hellman MD, et al. : Medial gastrocnemius flap in the course of treatment for an infection at the site of a total knee arthroplasty. JBJS. Essent. Surg. Tech. 2017; 7 (2): e14. PubMed Abstract | Publisher Full Text | Free Full Text 12. Amin K, Dempsey M, Ghali S, et al. : Saving grace: distally pedicled gracilis muscular flap in lower limb salvage. BMJ Case Rep. 2014; 2014 : bcr2014205486–bcr2014205486. Publisher Full Text 13. Cavadas PC, Sanz-Giménez-Rico JR, Landín L, et al. : Segmental gracilis free flap based on secondary pedicles: anatomical study and clinical series. Plast. Reconstr. Surg. 2004; 114 (3): 684–691. PubMed Abstract | Publisher Full Text 14. Jung JA, Kim YW, Cheon YW: Reverse gracilis muscle flap: an alternative means of skin coverage for recurrent infection after TKA. Knee Surg. Sports Traumatol. Arthrosc. 2013; 21 (12): 2779–2783. PubMed Abstract | Publisher Full Text 15. Rajeshwari MS, Kumar BNR: An anatomical study of gracilis muscle and its vascular pedicles. Int. J. Anat. Res. 2015; 3 (4): 1685–1688. Publisher Full Text 16. Magden O, Tayfur V, Edizer M, et al. : Anatomy of gracilis muscle flap. J. Craniofac. Surg. 2010; 21 (6): 1948–1950. Publisher Full Text 17. Singh H, Kaur R, Gupta N: Morphometric study of gracilis muscle and its role in clinical reconstruction. J. Anat. Soc. India. 2011; 60 (2): 202–206. Publisher Full Text 18. Murlimanju BV: Morphometry of gracilis. Dataset. figshare. 2023. Publisher Full Text Comments on this article Comments (0) Version 2 VERSION 2 PUBLISHED 18 Apr 2024 ADD YOUR COMMENT Comment Author details Author details 1 Department of Anatomy, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, India Chettiar Ganesh Kumar Roles: Data Curation, Writing – Original Draft Preparation Rajanigandha Vadgaonkar Roles: Data Curation, Writing – Review & Editing M.D. Prameela Roles: Data Curation Vandana Blossom Roles: Data Curation B.V. Murlimanju Roles: Conceptualization, Data Curation, Formal Analysis, Investigation, Methodology, Project Administration, Writing – Original Draft Preparation Mamatha Tonse Roles: Data Curation Mangala M. Pai Roles: Writing – Review & Editing Competing interests No competing interests were disclosed. Grant information The author(s) declared that no grants were involved in supporting this work. Article Versions (2) version 2 Revised Published: 12 Nov 2025, 13:299 https://doi.org/10.12688/f1000research.144786.2 version 1 Published: 18 Apr 2024, 13:299 https://doi.org/10.12688/f1000research.144786.1 Copyright © 2025 Kumar CG et al . This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Download Export To Sciwheel Bibtex EndNote ProCite Ref. Manager (RIS) Sente metrics Views Downloads F1000Research - - PubMed Central info_outline Data from PMC are received and updated monthly. - - Citations open_in_new 0 open_in_new 0 open_in_new SEE MORE DETAILS CITE how to cite this article Kumar CG, Vadgaonkar R, Prameela MD et al. Morphology of gracilis muscle and the topographic anatomy of its neurovascular pedicles: A cross sectional study [version 2; peer review: 2 approved] . F1000Research 2025, 13 :299 ( https://doi.org/10.12688/f1000research.144786.2 ) NOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS track receive updates on this article Track an article to receive email alerts on any updates to this article. TRACK THIS ARTICLE Share Open Peer Review Current Reviewer Status: ? Key to Reviewer Statuses VIEW HIDE Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions Version 2 VERSION 2 PUBLISHED 12 Nov 2025 Revised Views 0 Cite How to cite this report: Pushpa N. Reviewer Report For: Morphology of gracilis muscle and the topographic anatomy of its neurovascular pedicles: A cross sectional study [version 2; peer review: 2 approved] . F1000Research 2025, 13 :299 ( https://doi.org/10.5256/f1000research.190994.r432307 ) The direct URL for this report is: https://f1000research.com/articles/13-299/v2#referee-response-432307 NOTE: it is important to ensure the information in square brackets after the title is included in this citation. Close Copy Citation Details Reviewer Report 29 Dec 2025 Nagavalli Pushpa , Department of Anatomy, JSS Medical College, JSS Academy of Higher Education and Research, Mysore, Karnataka, India Approved VIEWS 0 https://doi.org/10.5256/f1000research.190994.r432307 Dear Author, In the introduction please add why Gracilis is the choice for graft. Please provide reference for the methodology. Mention how did you arrive at the present sample size. Kindly add some more points in the ... Continue reading READ ALL Dear Author, In the introduction please add why Gracilis is the choice for graft. Please provide reference for the methodology. Mention how did you arrive at the present sample size. Kindly add some more points in the discussion and make it robust. Manuscript is an useful study indicating the morphometry of the Gracilis. Is the work clearly and accurately presented and does it cite the current literature? Yes Is the study design appropriate and is the work technically sound? Yes Are sufficient details of methods and analysis provided to allow replication by others? Yes If applicable, is the statistical analysis and its interpretation appropriate? No Are all the source data underlying the results available to ensure full reproducibility? Yes Are the conclusions drawn adequately supported by the results? Yes Competing Interests: No competing interests were disclosed. Reviewer Expertise: Gross anatomy, Histology, Neuroanatomy and embryology. I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. Close READ LESS CITE CITE HOW TO CITE THIS REPORT Pushpa N. Reviewer Report For: Morphology of gracilis muscle and the topographic anatomy of its neurovascular pedicles: A cross sectional study [version 2; peer review: 2 approved] . F1000Research 2025, 13 :299 ( https://doi.org/10.5256/f1000research.190994.r432307 ) The direct URL for this report is: https://f1000research.com/articles/13-299/v2#referee-response-432307 NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS Report a concern Author Response 07 Jan 2026 B.V. Murlimanju , Department of Anatomy, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, India 07 Jan 2026 Author Response Dear Doctor, Thank you so much for your expert comments and for endorsing the significance of this anatomical study. Very much appreciated. Competing Interests: No competing interests were disclosed. Dear Doctor, Thank you so much for your expert comments and for endorsing the significance of this anatomical study. Very much appreciated. Dear Doctor, Thank you so much for your expert comments and for endorsing the significance of this anatomical study. Very much appreciated. Competing Interests: No competing interests were disclosed. Close Report a concern Respond or Comment COMMENTS ON THIS REPORT Author Response 07 Jan 2026 B.V. Murlimanju , Department of Anatomy, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, India 07 Jan 2026 Author Response Dear Doctor, Thank you so much for your expert comments and for endorsing the significance of this anatomical study. Very much appreciated. Competing Interests: No competing interests were disclosed. Dear Doctor, Thank you so much for your expert comments and for endorsing the significance of this anatomical study. Very much appreciated. Dear Doctor, Thank you so much for your expert comments and for endorsing the significance of this anatomical study. Very much appreciated. Competing Interests: No competing interests were disclosed. Close Report a concern COMMENT ON THIS REPORT Views 0 Cite How to cite this report: Patra A. Reviewer Report For: Morphology of gracilis muscle and the topographic anatomy of its neurovascular pedicles: A cross sectional study [version 2; peer review: 2 approved] . F1000Research 2025, 13 :299 ( https://doi.org/10.5256/f1000research.190994.r432309 ) The direct URL for this report is: https://f1000research.com/articles/13-299/v2#referee-response-432309 NOTE: it is important to ensure the information in square brackets after the title is included in this citation. Close Copy Citation Details Reviewer Report 26 Dec 2025 Apurba Patra , All India Institute of Medical Sciences, Bathinda, Punjab, India Approved VIEWS 0 https://doi.org/10.5256/f1000research.190994.r432309 The present artile describing thet morphology of gracilis muscle and the topographic anatomy of its neurovascular pedicles: A cross sectional study is very well executed and well written. This work bridges the gap in the existing literature regrading the morphology and ... Continue reading READ ALL The present artile describing thet morphology of gracilis muscle and the topographic anatomy of its neurovascular pedicles: A cross sectional study is very well executed and well written. This work bridges the gap in the existing literature regrading the morphology and topographic location of gracilis muscle, commonly used as a graft. Is the work clearly and accurately presented and does it cite the current literature? Yes Is the study design appropriate and is the work technically sound? Yes Are sufficient details of methods and analysis provided to allow replication by others? Yes If applicable, is the statistical analysis and its interpretation appropriate? Yes Are all the source data underlying the results available to ensure full reproducibility? Yes Are the conclusions drawn adequately supported by the results? Yes Competing Interests: No competing interests were disclosed. Reviewer Expertise: Translational research in Morphology, Neuroanatomy I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. Close READ LESS CITE CITE HOW TO CITE THIS REPORT Patra A. Reviewer Report For: Morphology of gracilis muscle and the topographic anatomy of its neurovascular pedicles: A cross sectional study [version 2; peer review: 2 approved] . F1000Research 2025, 13 :299 ( https://doi.org/10.5256/f1000research.190994.r432309 ) The direct URL for this report is: https://f1000research.com/articles/13-299/v2#referee-response-432309 NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article. COPY CITATION DETAILS Report a concern Author Response 07 Jan 2026 B.V. Murlimanju , Department of Anatomy, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, India 07 Jan 2026 Author Response Dear Doctor, Thank you so much for your expert comments and for endorsing the significance of this anatomical study. Very much appreciated. Competing Interests: No competing interests were disclosed. Dear Doctor, Thank you so much for your expert comments and for endorsing the significance of this anatomical study. Very much appreciated. Dear Doctor, Thank you so much for your expert comments and for endorsing the significance of this anatomical study. Very much appreciated. Competing Interests: No competing interests were disclosed. Close Report a concern Respond or Comment COMMENTS ON THIS REPORT Author Response 07 Jan 2026 B.V. Murlimanju , Department of Anatomy, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, India 07 Jan 2026 Author Response Dear Doctor, Thank you so much for your expert comments and for endorsing the significance of this anatomical study. Very much appreciated. Competing Interests: No competing interests were disclosed. Dear Doctor, Thank you so much for your expert comments and for endorsing the significance of this anatomical study. Very much appreciated. Dear Doctor, Thank you so much for your expert comments and for endorsing the significance of this anatomical study. Very much appreciated. Competing Interests: No competing interests were disclosed. Close Report a concern COMMENT ON THIS REPORT Comments on this article Comments (0) Version 2 VERSION 2 PUBLISHED 18 Apr 2024 ADD YOUR COMMENT Comment keyboard_arrow_left keyboard_arrow_right Open Peer Review Reviewer Status info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions Reviewer Reports Invited Reviewers 1 2 Version 2 (revision) 12 Nov 25 read read Version 1 18 Apr 24 Apurba Patra , All India Institute of Medical Sciences, Bathinda, India Nagavalli Pushpa , Department of Anatomy, JSS Medical College, JSS Academy of Higher Education and Research, Mysore, India Comments on this article All Comments (0) Add a comment Sign up for content alerts Sign Up You are now signed up to receive this alert Browse by related subjects keyboard_arrow_left Back to all reports Reviewer Report 0 Views copyright © 2026 Pushpa N. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 29 Dec 2025 | for Version 2 Nagavalli Pushpa , Department of Anatomy, JSS Medical College, JSS Academy of Higher Education and Research, Mysore, Karnataka, India 0 Views copyright © 2026 Pushpa N. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. format_quote Cite this report speaker_notes Responses (1) Approved info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions Dear Author, In the introduction please add why Gracilis is the choice for graft. Please provide reference for the methodology. Mention how did you arrive at the present sample size. Kindly add some more points in the discussion and make it robust. Manuscript is an useful study indicating the morphometry of the Gracilis. Is the work clearly and accurately presented and does it cite the current literature? Yes Is the study design appropriate and is the work technically sound? Yes Are sufficient details of methods and analysis provided to allow replication by others? Yes If applicable, is the statistical analysis and its interpretation appropriate? No Are all the source data underlying the results available to ensure full reproducibility? Yes Are the conclusions drawn adequately supported by the results? Yes Competing Interests No competing interests were disclosed. Reviewer Expertise Gross anatomy, Histology, Neuroanatomy and embryology. I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. reply Respond to this report Responses (1) Author Response 07 Jan 2026 B.V. Murlimanju, Department of Anatomy, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, India Dear Doctor, Thank you so much for your expert comments and for endorsing the significance of this anatomical study. Very much appreciated. View more View less Competing Interests No competing interests were disclosed. reply Respond Report a concern Pushpa N. Peer Review Report For: Morphology of gracilis muscle and the topographic anatomy of its neurovascular pedicles: A cross sectional study [version 2; peer review: 2 approved] . F1000Research 2025, 13 :299 ( https://doi.org/10.5256/f1000research.190994.r432307) NOTE: it is important to ensure the information in square brackets after the title is included in this citation. The direct URL for this report is: https://f1000research.com/articles/13-299/v2#referee-response-432307 keyboard_arrow_left Back to all reports Reviewer Report 0 Views copyright © 2025 Patra A. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 26 Dec 2025 | for Version 2 Apurba Patra , All India Institute of Medical Sciences, Bathinda, Punjab, India 0 Views copyright © 2025 Patra A. This is an open access peer review report distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. format_quote Cite this report speaker_notes Responses (1) Approved info_outline Alongside their report, reviewers assign a status to the article: Approved The paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. Not approved Fundamental flaws in the paper seriously undermine the findings and conclusions The present artile describing thet morphology of gracilis muscle and the topographic anatomy of its neurovascular pedicles: A cross sectional study is very well executed and well written. This work bridges the gap in the existing literature regrading the morphology and topographic location of gracilis muscle, commonly used as a graft. Is the work clearly and accurately presented and does it cite the current literature? Yes Is the study design appropriate and is the work technically sound? Yes Are sufficient details of methods and analysis provided to allow replication by others? Yes If applicable, is the statistical analysis and its interpretation appropriate? Yes Are all the source data underlying the results available to ensure full reproducibility? Yes Are the conclusions drawn adequately supported by the results? Yes Competing Interests No competing interests were disclosed. Reviewer Expertise Translational research in Morphology, Neuroanatomy I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. reply Respond to this report Responses (1) Author Response 07 Jan 2026 B.V. Murlimanju, Department of Anatomy, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, India Dear Doctor, Thank you so much for your expert comments and for endorsing the significance of this anatomical study. Very much appreciated. View more View less Competing Interests No competing interests were disclosed. reply Respond Report a concern Patra A. Peer Review Report For: Morphology of gracilis muscle and the topographic anatomy of its neurovascular pedicles: A cross sectional study [version 2; peer review: 2 approved] . F1000Research 2025, 13 :299 ( https://doi.org/10.5256/f1000research.190994.r432309) NOTE: it is important to ensure the information in square brackets after the title is included in this citation. The direct URL for this report is: https://f1000research.com/articles/13-299/v2#referee-response-432309 Alongside their report, reviewers assign a status to the article: Approved - the paper is scientifically sound in its current form and only minor, if any, improvements are suggested Approved with reservations - A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit. 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