The Role of Neutrophyl-to-lymphocyte Ratio as a Predictor of Orchiectomy or Testicular Atrophy After Torsion in Children. A Multicentric Study

The Role of Neutrophyl-to-lymphocyte Ratio as a Predictor of Orchiectomy or Testicular Atrophy After Torsion in Children. 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A Multicentric Study Carlos Delgado-Miguel, Javier Arredondo-Montero, Julio César Moreno-Alfonso, and 10 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5700353/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Introduction : Neutrophil-to-lymphocyte ratio (NLR) is an inflammatory biomarker (hemogram-derived-ratio) related to ischemic-inflammatory diseases. Its usefulness in the diagnosis of pediatric testicular torsion (TT) has recently been reported, although its prognostic implication has not been evaluated. Our aim is to analyze the role of NLR in the evolution of TT in children, determining its potential for predicting the risk of adverse outcomes such as orchiectomy or testicular atrophy. Methods : We performed a retrospective multicentric case-control study in patients with clinical and ultrasound suspicion of TT, in whom surgical testicular examination was performed between 2016-2022 in seven pediatric hospitals. Patient’s outcomes were analyzed according to the intraoperative and postoperative evolution (orchiectomy/testicular atrophy or not). Demographics, clinical, ultrasound and laboratory features at admission were analyzed. Sensitivity and specificity were determined by the area under the curve (AUC) represented on the receiver operating characteristic (ROC) curves. Results : A total of 455 patients (median age 13.2 years; interquartile range 10.6-14.4 years) were included, in whom 87 orchiectomies (19.1%) were performed and 34 cases of testicular atrophy (7.5%) were observed during follow-up (median follow-up: 10 months). When comparing clinical, ultrasound and laboratory predictors of both events on ROC curves, NLR was the most sensitive and specific parameter for predicting orchiectomy (AUC=0.834; p<0.001), as well as testicular atrophy (AUC=0.849; p<0.001). Compared with other parameters, the designed cut-off point of NLR=5.2 had maximum sensitivity and specificity (82.2% and 77.0%, respectively) for predicting orchiectomy or atrophy after testicular torsion. Conclusion : NLR may be considered as the best predictor for the risk of orchiectomy or testicular atrophy following torsion in pediatric patients, helping the identification of high-risk cases. It can be useful both for obtaining more accurate preoperative information on patient prognosis and for closer follow-up of high-risk testicular atrophy patients. Spermatic Cord Torsion Orchiectomy Testicular Atrophy Biomarkers Pediatrics Figures Figure 1 Figure 2 Figure 3 Introduction Testicular torsion (TT) is a common cause of acute scrotal pain in children and adolescents, which results in decreased blood flow to the testicle and requires urgent diagnosis and surgical treatment to avoid negative consequences ( 1 ). Twisting of the spermatic vessels leads to tissue ischemia and testicular necrosis, requiring excision of the testis (orchiectomy) in up to one-third of these patients ( 2 ). In some cases, despite reperfusion of the testis, testicular atrophy may be observed in the medium to long term. Several studies have identified certain clinical and intraoperative predictors of testicular viability following surgical detorsion, such as the duration of symptoms before surgery and the degree of testicular twisting ( 3 – 6 ). However, there are scarce studies investigating the prognostic value of laboratory parameters in TT among children, with the majority of research focusing on adult patients ( 7 – 9 ). Neutrophil-to-lymphocyte ratio (NLR) is an inflammatory hemogram-derived-ratio related to ischemic-inflammatory diseases in children such as intussusception or ovarian torsion ( 10 – 12 ). Its usefulness in TT diagnosis has recently been reported ( 13 ), although its prognostic implications have not been evaluated. The aim of this study is to analyse the role of NLR in the evolution of TT in children, determining its potential for predicting the risk of adverse outcomes such as orchiectomy or testicular atrophy. Methods A retrospective multicenter case-control study was performed in patients with clinical and ultrasound suspicion of TT, in whom surgical testicular exploration was performed at seven pediatric institutions between January 2016 and December 2022. Patients´ outcomes were analysed according to the intraoperative and postoperative evolution (orchiectomy/testicular atrophy or not). Demographic information, clinical presentation, color-Doppler ultrasonography (CDUS) findings, and laboratory results upon admission were documented. Newborns with prenatal or neonatal testicular torsion were excluded, as well as patients with torted undescended testis and those with incomplete or missing data. Clinical features encompassed the duration of testicular pain, affected side, and symptoms such as abdominal pain, vomiting, dizziness, dysuria or fever (temperature ≥ 37.5°C). Physical examination included assessment of scrotal swelling, absence of cremasteric reflex, and the absence of Prehn's sign. Ultrasound results included the absence of intratesticular blood flow and testicular volumes, as well as the identification of the whirlpool sign, which is defined as a spiral twist of the spermatic cord ( 14 ). Testicular volumes were calculated using the ellipsoid formula: length (mm) × width (mm) × weight (mm) × 0.71 ( 15 ). Laboratory data were gathered from blood tests conducted in the Emergency Department upon the patients' arrival. These tests encompassed a complete blood count, including leukocyte count and absolute counts of neutrophils, lymphocytes, monocytes, basophils, and eosinophils. Additionally, biochemistry parameters such as glucose, fibrinogen, ion levels and C-reactive protein (CRP) levels were measured. NLR was calculated by dividing the absolute number of neutrophils by the absolute number of lymphocytes. Platelet-to-Lymphocyte Ratio (PLR) was obtained by the ratio between the total number of platelets (x10 9 /L) and lymphocytes (x10 9 /L). SIRI, Systemic Inflammation Response Index was obtained by the following formula: neutrophil × monocytes / lymphocytes. Systemic Immune-Inflammation Index (SII) was obtained by calculating neutrophil × platelets / lymphocytes. Intraoperative findings included the diagnosis of TT, the direction of rotation, the degree of twisting cord (DTC), as well as subsequent testicular viability. Orchiectomy was performed in those cases of non-recoverable testicular necrosis after detorsion. Follow-up clinic notes were reviewed for the presence of testicular atrophy after orchiopexy. Testicular atrophy was defined by new reduction in size in comparison with contralateral testicle found on clinical examination and/or Doppler ultrasound (defined as a > 50% difference in volume when compared to the contralateral testis based on obvious physical examination findings, orchidometry, or sonographic measurements, depending on the clinician’s preference ( 16 , 17 ). The study protocol adhered to the principles outlined in the Declaration of Helsinki (2013 revision). Approval was obtained from the hospital's institutional review board before conducting this retrospective data analysis (IRB number PI-263-23). Written informed consent was deemed unnecessary given the retrospective nature of the study, the absence of human or animal samples, and the anonymous collection of analytical data, all in accordance with institutional guidelines. Data were collected using Microsoft Excel software version 2010 (Redmond, WA, USA) and analyzed with SPSS Statistics version 25 (Chicago, IL, USA). The normality of variables was assessed using Kolmogórov-Smirnov and Shapiro-Wilk tests. Continuous variables following a normal distribution were presented as mean and standard deviation (SD) and were compared using ANOVA test for independent samples. For continuous data not adhering to a normal distribution, median and interquartile range (IQR) were utilized, and the Kruskal–Wallis test was applied for analysis. Discrete variables were shown as frequency and percentage and analyzed using the Chi-square test or Fisher's test when applicable. Odds ratios (OR) with 95% confidence intervals were calculated. All statistical analyses were two-tailed, and significance was set at p < 0.05. Sensitivity and specificity for orchiectomy and testicular atrophy were evaluated using area under the curve (AUC) in receiver operating characteristic (ROC) curves. The DeLong method was used to compare these curves ( 18 ). Optimal cut-off values for maximal diagnostic accuracy of each analytical parameter were determined using the Youden index formula: "sensitivity + specificity – 1" ( 19 ). Results A total of 455 patients were included (median age 13.2 years; interquartile range 11.2-15.6 years), in whom 87 orchiectomies (21.6%) were performed and 34 (9.2%) cases of testicular atrophy were observed during follow-up (median follow-up: 32 months). Table 1 shows demographic, clinical and ultrasound features of the overall patient series, as well as data on patients with non-lost testes, those in whom orchiectomy was performed and those in whom testicular atrophy occurred despite testicular fixation after torsion. Patients who underwent orchiectomy were significantly younger than the other groups (p=0.018), and consequently had a lower weight. The left testicle was slightly more frequently affected in all groups. Vomiting and abdominal pain were the most commonly associated symptoms, followed by dizziness, fever and dysuria. On physical examination, scrotal swelling was the most common finding in more than half of the patients, while absence of the cremasteric reflex and a negative Prehn's sign were found in one third of cases. There were no differences in symptoms or clinical data between the groups. However, the time from symptom onset was significantly longer in orchiectomy patients (24 hours), and in those with testicular atrophy (8 hours) when compared to patients with long-term viable testicle (4 hours). Ultrasonographic data revealed an absence of testicular Doppler flow more frequently observed in patients with orchiectomy or atrophy (96% and 94% respectively) compared to 88% of patients with successful testicular evolution (p=0.013). No differences were observed in the detection of the whirling sign or testicular volume. Regarding intraoperative findings, the median number of twisting degrees was higher in patients with unfavourable evolution (720 degrees) compared to 360 degrees in patients without testicular loss (<0.001). Laboratory data at admission in the 3 groups is shown in Table 2. We observed that patients with orchiectomy or atrophy had significantly higher values of leukocytes, neutrophils, as well as inflammatory indices (NLR, PLR, SIRI and IBS) and CRP. There were no differences in biochemistry values or electrolytes. When analysing quantitative risk factors for orchiectomy in a multivariate ROC curve analysis, we found that NLR was the parameter with the highest AUC (0.834) and the highest sensitivity and specificity for predicting orchiectomy (78.5% and 77.4% respectively, with a cut-off point of 5.1), followed by time since symptoms onset (AUC=0.820; 76.1% sensitivity and 78.0% specificity). Figure 1 and Table 3 show the AUC values of the different parameters studied for orchiectomy prediction. When performing the sensitivity and specificity analysis using ROC curves for the development of testicular atrophy after detorsion, we observed that NLR was the parameter with the highest AUC (0.849), which was significantly higher than time since symptoms onset, degrees of twisting cord, age at torsion and the rest of the laboratory parameters analysed. A sensitivity and specificity of 88.9% and 77.4% was established for the cut-off point of NLR >5.4. Detailed results, including ROC curves and the corresponding sensitivity and specificity values, as well as the determined cut-off points for the analyzed parameters, are presented in Figure 2 and Table 4. Finally, when analysing the risk of testicular loss (both orchiectomy and testicular atrophy together), we observed that the inflammatory parameters were the ones that obtained the highest AUC when compared with clinical or intraoperative data (Figure 3 and Table 5). Once again, NLR was the best predictor of unfavourable outcome (AUC=0.838) with the highest sensitivity (82.2%) and specificity (77.0%) for the cut-off point of 5.2, followed by SII (AUC=0.817; 78.1% sensitivity and 74% specificity for the cut-off point of 1224). Discussion This study analyses for the first time the role of NLR and other inflammatory laboratory parameters as predictors of orchiectomy or testicular atrophy in boys after testicular torsion. Achieving an accurate and prompt diagnosis followed by urgent surgical intervention is imperative to prevent irreversible damage and potential loss of the testis ( 20 , 21 ). Testicular torsion can manifest at any age, but it predominantly occurs after the age of 10, peaking between 12 and 16 years ( 22 ). In our study, the median age was 13 years, and we observed a slight left-sided predominance, aligning with previous research findings ( 1 , 2 ). This condition not only represents a significant cause of testicular loss in children but also carries the risk of impacting future fertility and testicular endocrine function, as well as psychological distress associated. In patients with testicular torsion, surgeons must carefully evaluate the potential complications associated with testicular preservation such as infection, malignant transformation and atrophy, against those linked to testicular removal, including trauma and psychosocial challenges during puberty. Although these risks are generally acknowledged preoperatively, the overall rate of orchiectomy following surgical detorsion remains notably high, as does the incidence of testicular atrophy after emergent orchiopexy. Consequently, identifying preoperative or intraoperative prognostic factors is crucial to optimize surgical planning and providing accurate information to patients and their parents. In our multicenter series, the testicular loss rate of 30.8% (21.6% orchiectomy rate and 9.2% TT rate) was similar to that described by other authors ( 16 , 23 ), though lower than that reported by other authors with series covering a greater number of years. For instance, Tian et al. reviewed 113 cases of testicular torsion treated at their center over a ten-year period and reported an orchiectomy rate of 44.4%, which increased to 65% when cases of testicular atrophy were included ( 24 ). Yu et al. described an orchectomy and TA rates of 26.9% and 56.6%, respectively, in 145 patients operated on over a 16-year interval ( 25 ). These differences in atrophy rate may be due to the different definition of TA during recent years, which sometimes might be overestimated in series that include patients over more than 10 years of time. An extended duration between the onset of pain and the diagnosis of torsion is associated with a reduced likelihood of testicular salvage ( 15 ). In a retrospective analysis, Lian et al. reported an increased occurrence of postoperative testicular atrophy associated with delays exceeding 24 hours ( 26 ). Later, Tian et al. observed that delaying surgery beyond 12 hours significantly increased testicular volume loss compared to operations performed within 12 hours. Additionally, postponing surgery beyond 24 hours was strongly linked to a higher risk of testicular atrophy ( 24 ). However, the exact determination of the time interval between initial symptoms and presentation to the Emergency Department remains challenging. The low accuracy in recalling the exact onset of symptoms, combined with delays in seeking medical assistance by children and adolescents, can distort the actual timeframe from the start of testicular ischemia. Furthermore, not all cases of TT present with acute, intense scrotal pain. Some patients exhibit variable initial symptoms, including discomfort localized to the lower abdomen or inguinal region ( 27 ). In addition to delayed surgery, the degree of torsion has also been recognized as a key prognostic factor for testicular viability during surgical intervention ( 16 , 28 ). Several authors have reported a correlation between a higher degree of testicular rotation and an increased likelihood of undergoing orchidectomy compared to orchiopexy ( 5 , 29 ). While lower degrees of torsion may allow partial restoration of blood flow to the testicle, leading surgeons to opt for testis-salvaging procedures in hopes of preserving function and delaying orchiectomy unless absolutely necessary, many testicles still experience substantial atrophy when monitored over the long term in clinical follow-up ( 16 ). Chen et al. proposed a nomogram to estimate the likelihood of testicular salvage based on factors such as symptom duration, intratesticular blood flow, degree of spermatic cord torsion, and monocyte count. However, the subjective variability of certain parameters and the limited sample size pose challenges for generalizing these findings ( 30 ). Howe et al. identified cut-off values for testicular loss at 8.5 hours and 495 degrees of torsion, offering sensitivities of 73% and 53%, respectively, with a specificity of 80% for both thresholds ( 16 ). Some authors have described other clinical or ultrasound predictors of TA such as red scrotal changes or heterogeneous testis parenchyma on ultrasound, but no objective laboratory parameters such as those included in our study have been analyzed to date ( 26 , 31 ). We had previously described the usefulness of NLR in the diagnosis of TT, and we have recently observed that this marker is also useful in diagnosis of ovarian torsion in pediatric population ( 13 ). However, its usefulness as a prognostic marker had not been explored until now. This study allows us to broaden the application of NLR not only as a diagnostic marker but also as a prognostic marker of TT. The inflammatory response triggered by testicular ischemia results in neutrophilia, driven by chemotaxis and increased release of neutrophils from the bone marrow into peripheral circulation, along with lymphopenia caused by elevated endogenous cortisol levels induced by ischemia ( 13 ). These combined mechanisms lead to an increase in the NLR through two distinct pathways. This could account for the higher AUC observed for NLR compared to other inflammatory markers like PLR, SIRI, or SII, which involve monocytes or platelets, as NLR reflects the synergistic cellular response of neutrophilia and lymphopenia more directly. This systemic inflammation observed in the laboratory study of patients with suspected TT thus provides prognostic information on the evolution and repercussions of ischemia in the short and medium term, such as orchiectomy or testicular atrophy, and makes it possible to predict them more accurately than clinical or intraoperative data. This study included a substantial participant pool, offering significant insights into the enduring outcomes of pediatric patients with TT. In addition, it analyses the predictive role of different clinical parameters (age at torsion, time since symptom onset, degree of twisting cord), together with laboratory data commonly used in clinical practice, all derived from the haemogram. Furthermore, it examines for the first time several inflammatory markers, including NLR, PLR, SIRI, and SII, which can be easily derived from routine blood tests, and to compare these markers with clinical and ultrasound findings, mimicking everyday clinical scenarios. The results highlight NLR as the most accurate and reliable indicator for predicting testicular loss either by orchiectomy or testicular atrophy in male children after testicular torsion. This parameter is more consistent than clinical observations, such as the duration of symptoms, which can be unreliable due to the gradual onset of pain in some instances. Furthermore, NLR eliminates the variability associated with radiological evaluations, such as ultrasound, which can depend heavily on the examiner's expertise. All this facilitates the practical use of the findings on a day-to-day basis, which makes them highly applicable in clinical practice. However, there are several limitations to consider. Firstly, the retrospective design confines the analysis to data already recorded in medical records, potentially introducing recall and selection biases. Additionally, the lack of similar studies in pediatric patients complicates direct comparison of results. Another limitation is the variability in ultrasound imaging, as it was not consistently performed by the same radiologist, which may affect the accuracy of testicular volume calculations, Doppler flow identification or whirlpool sign visualization. Furthermore, the study did not explore the impact of testicular atrophy on hormone production, semen quality and fertility, which added to the relatively short-term follow-up makes that long-term outcomes such as testicular atrophy and infertility may not be fully captured. It is possible that testicular development during puberty could reveal significant volume loss in pre-pubertal boys who experienced testicular torsion. Therefore, caution is advised when generalizing these findings, and further prospective studies with longer follow-up periods are still needed to validate these results. Conclusion The neutrophil-to-lymphocyte ratio (NLR) can be considered the most reliable predictor of the risk of orchiectomy or testicular atrophy following torsion in pediatric patients, aiding in the identification of high-risk cases. It may prove useful both for obtaining more accurate preoperative information regarding the patient's prognosis and for closer monitoring of patients at elevated risk of testicular atrophy. Declarations Conflicts of interest : The authors of this manuscript do not refer to any conflict of interest. Funding : This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors. Ethics approval : The study protocol obtained the approval of Fundación Jiménez Díaz Hospital Institutional Review Board (IRB number PI-264-23) and complied with the guidelines of the Declaration of Helsinki (as revised in 2013). References Drlík M, Kočvara R. Torsion of spermatic cord in children: a review. 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Delgado-Miguel C, Arredondo-Montero J, Moreno-Alfonso JC, San Basilio M, Peña Pérez R, Carrera N, et al. The Role of Neutrophyl-to-Lymphocyte Ratio as a Predictor of Ovarian Torsion in Children: Results of a Multicentric Study. Life (Basel). 2024;14(7):889. Tables Tables 1 to 5 are available in the Supplementary Files section Additional Declarations No competing interests reported. Supplementary Files Table1.docx Table2.docx Table3.docx Table4.docx Table5.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5700353","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":396177951,"identity":"05f9591a-7567-4686-90a7-57962de51235","order_by":0,"name":"Carlos 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Hospital","correspondingAuthor":false,"prefix":"","firstName":"Estíbalitz","middleName":"","lastName":"Iraola","suffix":""},{"id":396177959,"identity":"bbe4416b-6a96-48fd-be24-90129d863884","order_by":8,"name":"Inmaculada Ruiz Jiménez","email":"","orcid":"","institution":"Villalba University Hospital","correspondingAuthor":false,"prefix":"","firstName":"Inmaculada","middleName":"Ruiz","lastName":"Jiménez","suffix":""},{"id":396177960,"identity":"619f7004-edfb-4386-a3d6-debfe021b813","order_by":9,"name":"Pablo Aguado Roncero","email":"","orcid":"","institution":"Fundación Jiménez Díaz University Hospital","correspondingAuthor":false,"prefix":"","firstName":"Pablo","middleName":"Aguado","lastName":"Roncero","suffix":""},{"id":396177961,"identity":"45afb253-2d41-4eca-9364-eb368692cf43","order_by":10,"name":"Ennio Fuentes","email":"","orcid":"","institution":"Fundación Jiménez Díaz University Hospital","correspondingAuthor":false,"prefix":"","firstName":"Ennio","middleName":"","lastName":"Fuentes","suffix":""},{"id":396177962,"identity":"1208d13e-61a5-4489-9106-d0d51ee83093","order_by":11,"name":"Ricardo Díez","email":"","orcid":"","institution":"Fundación Jiménez Díaz University Hospital","correspondingAuthor":false,"prefix":"","firstName":"Ricardo","middleName":"","lastName":"Díez","suffix":""},{"id":396177963,"identity":"b6d52f25-c618-4eda-93f8-549135e4ffbd","order_by":12,"name":"Francisco Hernández-Oliveros","email":"","orcid":"","institution":"La Paz University Hospital","correspondingAuthor":false,"prefix":"","firstName":"Francisco","middleName":"","lastName":"Hernández-Oliveros","suffix":""}],"badges":[],"createdAt":"2024-12-23 14:23:11","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-5700353/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-5700353/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":72738592,"identity":"d8a38061-4760-4e85-9f17-856ddd142247","added_by":"auto","created_at":"2025-01-01 09:13:59","extension":"jpg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":24919,"visible":true,"origin":"","legend":"\u003cp\u003eROC curve for orchiectomy risk after testicular torsion. TSSO, time since symptoms onset; DTC, degree of twisted cord; NTC, neutrophils total count; NLR, Neutrophil-to-Lymphocyte Ratio; PLR, Platelet-to-Lymphocyte Ratio; SIRI, Systemic Inflammation Response Index; SII, Systemic Immune-Inflammation Index; CRP, C-reactive protein.\u003c/p\u003e","description":"","filename":"Figure1.jpg","url":"https://assets-eu.researchsquare.com/files/rs-5700353/v1/7176e36be28de61686f7b58c.jpg"},{"id":72740875,"identity":"5687affd-7223-42c0-8f99-40212367c640","added_by":"auto","created_at":"2025-01-01 09:37:59","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":26500,"visible":true,"origin":"","legend":"\u003cp\u003eROC curve for testicular atrophy risk after detorsion. NLR, Neutrophil-to-Lymphocyte Ratio; TSSO, time since symptoms onset; SII, Systemic Immune-Inflammation Index; SIRI, Systemic Inflammation Response Index; NTC, neutrophils total count; WBC, White Blood Cells; PLR, Platelet-to-Lymphocyte Ratio; DTC, Degree of Twisted Cord; CRP, C-reactive protein.\u003c/p\u003e","description":"","filename":"Figure2.jpg","url":"https://assets-eu.researchsquare.com/files/rs-5700353/v1/5f331b6fada07af9ea618314.jpg"},{"id":72738593,"identity":"50424643-3f03-42ea-b143-d00a6e44b1a6","added_by":"auto","created_at":"2025-01-01 09:13:59","extension":"jpg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":28704,"visible":true,"origin":"","legend":"\u003cp\u003eROC curve for testicular loss (orchiectomy or testicular atrophy) after testicular torsion. NLR, Neutrophil-to-Lymphocyte Ratio; TSSO, time since symptoms onset; SII, Systemic Immune-Inflammation Index; SIRI, Systemic Inflammation Response Index; NTC, neutrophils total count; WBC, White Blood Cells; PLR, Platelet-to-Lymphocyte Ratio; DTC, Degree of Twisted Cord; CRP, C-reactive protein.\u003c/p\u003e","description":"","filename":"Figure3.jpg","url":"https://assets-eu.researchsquare.com/files/rs-5700353/v1/ba8968a8cd6aeb370fa7edd5.jpg"},{"id":72740876,"identity":"742f3fed-06f8-4cbc-aa34-b1aee8ba2f6f","added_by":"auto","created_at":"2025-01-01 09:38:03","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":416131,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-5700353/v1/13027102-0df7-47b8-97d8-091826194507.pdf"},{"id":72738591,"identity":"d6440210-2f10-4975-8050-80287e44f16d","added_by":"auto","created_at":"2025-01-01 09:13:59","extension":"docx","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":18183,"visible":true,"origin":"","legend":"","description":"","filename":"Table1.docx","url":"https://assets-eu.researchsquare.com/files/rs-5700353/v1/80943f48a5ac069f09403795.docx"},{"id":72740209,"identity":"9439337b-1d4b-4664-a470-615675fdba62","added_by":"auto","created_at":"2025-01-01 09:29:59","extension":"docx","order_by":2,"title":"","display":"","copyAsset":false,"role":"supplement","size":18779,"visible":true,"origin":"","legend":"","description":"","filename":"Table2.docx","url":"https://assets-eu.researchsquare.com/files/rs-5700353/v1/05fe961f9d56c744eea3148a.docx"},{"id":72738598,"identity":"2e14bba4-d3c8-4c12-9ab8-8e91fd466b69","added_by":"auto","created_at":"2025-01-01 09:13:59","extension":"docx","order_by":3,"title":"","display":"","copyAsset":false,"role":"supplement","size":15364,"visible":true,"origin":"","legend":"","description":"","filename":"Table3.docx","url":"https://assets-eu.researchsquare.com/files/rs-5700353/v1/199b3fe6d5af9809dfc97936.docx"},{"id":72738594,"identity":"25d3da2f-e67e-48a5-aef3-68aeb81d049b","added_by":"auto","created_at":"2025-01-01 09:13:59","extension":"docx","order_by":4,"title":"","display":"","copyAsset":false,"role":"supplement","size":15358,"visible":true,"origin":"","legend":"","description":"","filename":"Table4.docx","url":"https://assets-eu.researchsquare.com/files/rs-5700353/v1/e9cb180939449e5e75a01cb5.docx"},{"id":72738602,"identity":"304d2c03-aef7-40b8-a33e-e1284a15dc8d","added_by":"auto","created_at":"2025-01-01 09:13:59","extension":"docx","order_by":5,"title":"","display":"","copyAsset":false,"role":"supplement","size":15741,"visible":true,"origin":"","legend":"","description":"","filename":"Table5.docx","url":"https://assets-eu.researchsquare.com/files/rs-5700353/v1/f144fa890429d76a07fc367e.docx"}],"financialInterests":"No competing interests reported.","formattedTitle":"\u003cp\u003eThe Role of Neutrophyl-to-lymphocyte Ratio as a Predictor of Orchiectomy or Testicular Atrophy After Torsion in Children. A Multicentric Study\u003c/p\u003e","fulltext":[{"header":"Introduction","content":"\u003cp\u003eTesticular torsion (TT) is a common cause of acute scrotal pain in children and adolescents, which results in decreased blood flow to the testicle and requires urgent diagnosis and surgical treatment to avoid negative consequences (\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e). Twisting of the spermatic vessels leads to tissue ischemia and testicular necrosis, requiring excision of the testis (orchiectomy) in up to one-third of these patients (\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e). In some cases, despite reperfusion of the testis, testicular atrophy may be observed in the medium to long term. Several studies have identified certain clinical and intraoperative predictors of testicular viability following surgical detorsion, such as the duration of symptoms before surgery and the degree of testicular twisting (\u003cspan additionalcitationids=\"CR4 CR5\" citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e). However, there are scarce studies investigating the prognostic value of laboratory parameters in TT among children, with the majority of research focusing on adult patients (\u003cspan additionalcitationids=\"CR8\" citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e).\u003c/p\u003e \u003cp\u003eNeutrophil-to-lymphocyte ratio (NLR) is an inflammatory hemogram-derived-ratio related to ischemic-inflammatory diseases in children such as intussusception or ovarian torsion (\u003cspan additionalcitationids=\"CR11\" citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e). Its usefulness in TT diagnosis has recently been reported (\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e), although its prognostic implications have not been evaluated. The aim of this study is to analyse the role of NLR in the evolution of TT in children, determining its potential for predicting the risk of adverse outcomes such as orchiectomy or testicular atrophy.\u003c/p\u003e"},{"header":"Methods","content":"\u003cp\u003eA retrospective multicenter case-control study was performed in patients with clinical and ultrasound suspicion of TT, in whom surgical testicular exploration was performed at seven pediatric institutions between January 2016 and December 2022. Patients\u0026acute; outcomes were analysed according to the intraoperative and postoperative evolution (orchiectomy/testicular atrophy or not).\u003c/p\u003e \u003cp\u003eDemographic information, clinical presentation, color-Doppler ultrasonography (CDUS) findings, and laboratory results upon admission were documented. Newborns with prenatal or neonatal testicular torsion were excluded, as well as patients with torted undescended testis and those with incomplete or missing data. Clinical features encompassed the duration of testicular pain, affected side, and symptoms such as abdominal pain, vomiting, dizziness, dysuria or fever (temperature\u0026thinsp;\u0026ge;\u0026thinsp;37.5\u0026deg;C). Physical examination included assessment of scrotal swelling, absence of cremasteric reflex, and the absence of Prehn's sign. Ultrasound results included the absence of intratesticular blood flow and testicular volumes, as well as the identification of the whirlpool sign, which is defined as a spiral twist of the spermatic cord (\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e). Testicular volumes were calculated using the ellipsoid formula: length (mm) \u0026times; width (mm) \u0026times; weight (mm) \u0026times; 0.71 (\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e).\u003c/p\u003e \u003cp\u003eLaboratory data were gathered from blood tests conducted in the Emergency Department upon the patients' arrival. These tests encompassed a complete blood count, including leukocyte count and absolute counts of neutrophils, lymphocytes, monocytes, basophils, and eosinophils. Additionally, biochemistry parameters such as glucose, fibrinogen, ion levels and C-reactive protein (CRP) levels were measured. NLR was calculated by dividing the absolute number of neutrophils by the absolute number of lymphocytes. Platelet-to-Lymphocyte Ratio (PLR) was obtained by the ratio between the total number of platelets (x10\u003csup\u003e9\u003c/sup\u003e/L) and lymphocytes (x10\u003csup\u003e9\u003c/sup\u003e/L). SIRI, Systemic Inflammation Response Index was obtained by the following formula: neutrophil \u0026times; monocytes / lymphocytes. Systemic Immune-Inflammation Index (SII) was obtained by calculating neutrophil \u0026times; platelets / lymphocytes.\u003c/p\u003e \u003cp\u003eIntraoperative findings included the diagnosis of TT, the direction of rotation, the degree of twisting cord (DTC), as well as subsequent testicular viability. Orchiectomy was performed in those cases of non-recoverable testicular necrosis after detorsion. Follow-up clinic notes were reviewed for the presence of testicular atrophy after orchiopexy. Testicular atrophy was defined by new reduction in size in comparison with contralateral testicle found on clinical examination and/or Doppler ultrasound (defined as a\u0026thinsp;\u0026gt;\u0026thinsp;50% difference in volume when compared to the contralateral testis based on obvious physical examination findings, orchidometry, or sonographic measurements, depending on the clinician\u0026rsquo;s preference (\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e, \u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e).\u003c/p\u003e \u003cp\u003e The study protocol adhered to the principles outlined in the Declaration of Helsinki (2013 revision). Approval was obtained from the hospital's institutional review board before conducting this retrospective data analysis (IRB number PI-263-23). Written informed consent was deemed unnecessary given the retrospective nature of the study, the absence of human or animal samples, and the anonymous collection of analytical data, all in accordance with institutional guidelines.\u003c/p\u003e \u003cp\u003eData were collected using Microsoft Excel software version 2010 (Redmond, WA, USA) and analyzed with SPSS Statistics version 25 (Chicago, IL, USA). The normality of variables was assessed using Kolmog\u0026oacute;rov-Smirnov and Shapiro-Wilk tests. Continuous variables following a normal distribution were presented as mean and standard deviation (SD) and were compared using ANOVA test for independent samples. For continuous data not adhering to a normal distribution, median and interquartile range (IQR) were utilized, and the Kruskal\u0026ndash;Wallis test was applied for analysis. Discrete variables were shown as frequency and percentage and analyzed using the Chi-square test or Fisher's test when applicable. Odds ratios (OR) with 95% confidence intervals were calculated. All statistical analyses were two-tailed, and significance was set at p\u0026thinsp;\u0026lt;\u0026thinsp;0.05. Sensitivity and specificity for orchiectomy and testicular atrophy were evaluated using area under the curve (AUC) in receiver operating characteristic (ROC) curves. The DeLong method was used to compare these curves (\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e). Optimal cut-off values for maximal diagnostic accuracy of each analytical parameter were determined using the Youden index formula: \"sensitivity\u0026thinsp;+\u0026thinsp;specificity \u0026ndash; 1\" (\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e).\u003c/p\u003e"},{"header":"Results","content":"\u003cp\u003eA total of 455 patients were included (median age 13.2 years; interquartile range 11.2-15.6 years), in whom 87 orchiectomies (21.6%) were performed and 34 (9.2%) cases of testicular atrophy were observed during follow-up (median follow-up: 32 months). Table 1 shows demographic, clinical and ultrasound features of the overall patient series, as well as data on patients with non-lost testes, those in whom orchiectomy was performed and those in whom testicular atrophy occurred despite testicular fixation after torsion. Patients who underwent orchiectomy were significantly younger than the other groups (p=0.018), and consequently had a lower weight. The left testicle was slightly more frequently affected in all groups. Vomiting and abdominal pain were the most commonly associated symptoms, followed by dizziness, fever and dysuria. On physical examination, scrotal swelling was the most common finding in more than half of the patients, while absence of the cremasteric reflex and a negative Prehn\u0026apos;s sign were found in one third of cases. There were no differences in symptoms or clinical data between the groups. However, the time from symptom onset was significantly longer in orchiectomy patients (24 hours), and in those with testicular atrophy (8 hours) when compared to patients with long-term viable testicle (4 hours). Ultrasonographic data revealed an absence of testicular Doppler flow more frequently observed in patients with orchiectomy or atrophy (96% and 94% respectively) compared to 88% of patients with successful testicular evolution (p=0.013). No differences were observed in the detection of the whirling sign or testicular volume. Regarding intraoperative findings, the median number of twisting degrees was higher in patients with unfavourable evolution (720 degrees) compared to 360 degrees in patients without testicular loss (\u0026lt;0.001).\u003c/p\u003e\n\u003cp\u003eLaboratory data at admission in the 3 groups is shown in Table 2. We observed that patients with orchiectomy or atrophy had significantly higher values of leukocytes, neutrophils, as well as inflammatory indices (NLR, PLR, SIRI and IBS) and CRP. There were no differences in biochemistry values or electrolytes. When analysing quantitative risk factors for orchiectomy in a multivariate ROC curve analysis, we found that NLR was the parameter with the highest AUC (0.834) and the highest sensitivity and specificity for predicting orchiectomy (78.5% and 77.4% respectively, with a cut-off point of 5.1), followed by time since symptoms onset (AUC=0.820; 76.1% sensitivity and 78.0% specificity). Figure 1 and Table 3 show the AUC values of the different parameters studied for orchiectomy prediction.\u003c/p\u003e\n\u003cp\u003eWhen performing the sensitivity and specificity analysis using ROC curves for the development of testicular atrophy after detorsion, we observed that NLR was the parameter with the highest AUC (0.849), which was significantly higher than time since symptoms onset, degrees of twisting cord, age at torsion and the rest of the laboratory parameters analysed. A sensitivity and specificity of 88.9% and 77.4% was established for the cut-off point of NLR \u0026gt;5.4. Detailed results, including ROC curves and the corresponding sensitivity and specificity values, as well as the determined cut-off points for the analyzed parameters, are presented in Figure 2 and Table 4.\u003c/p\u003e\n\u003cp\u003eFinally, when analysing the risk of testicular loss (both orchiectomy and testicular atrophy together), we observed that the inflammatory parameters were the ones that obtained the highest AUC when compared with clinical or intraoperative data (Figure 3 and Table 5). Once again, NLR was the best predictor of unfavourable outcome (AUC=0.838) with the highest sensitivity (82.2%) and specificity (77.0%) for the cut-off point of 5.2, followed by SII (AUC=0.817; 78.1% sensitivity and 74% specificity for the cut-off point of 1224).\u003c/p\u003e"},{"header":"Discussion","content":"\u003cp\u003eThis study analyses for the first time the role of NLR and other inflammatory laboratory parameters as predictors of orchiectomy or testicular atrophy in boys after testicular torsion. Achieving an accurate and prompt diagnosis followed by urgent surgical intervention is imperative to prevent irreversible damage and potential loss of the testis (\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e, \u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e). Testicular torsion can manifest at any age, but it predominantly occurs after the age of 10, peaking between 12 and 16 years (\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e). In our study, the median age was 13 years, and we observed a slight left-sided predominance, aligning with previous research findings (\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e, \u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e). This condition not only represents a significant cause of testicular loss in children but also carries the risk of impacting future fertility and testicular endocrine function, as well as psychological distress associated. In patients with testicular torsion, surgeons must carefully evaluate the potential complications associated with testicular preservation such as infection, malignant transformation and atrophy, against those linked to testicular removal, including trauma and psychosocial challenges during puberty. Although these risks are generally acknowledged preoperatively, the overall rate of orchiectomy following surgical detorsion remains notably high, as does the incidence of testicular atrophy after emergent orchiopexy. Consequently, identifying preoperative or intraoperative prognostic factors is crucial to optimize surgical planning and providing accurate information to patients and their parents.\u003c/p\u003e \u003cp\u003eIn our multicenter series, the testicular loss rate of 30.8% (21.6% orchiectomy rate and 9.2% TT rate) was similar to that described by other authors (\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e, \u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e23\u003c/span\u003e), though lower than that reported by other authors with series covering a greater number of years. For instance, Tian et al. reviewed 113 cases of testicular torsion treated at their center over a ten-year period and reported an orchiectomy rate of 44.4%, which increased to 65% when cases of testicular atrophy were included (\u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e). Yu et al. described an orchectomy and TA rates of 26.9% and 56.6%, respectively, in 145 patients operated on over a 16-year interval (\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e). These differences in atrophy rate may be due to the different definition of TA during recent years, which sometimes might be overestimated in series that include patients over more than 10 years of time.\u003c/p\u003e \u003cp\u003eAn extended duration between the onset of pain and the diagnosis of torsion is associated with a reduced likelihood of testicular salvage (\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e). In a retrospective analysis, Lian et al. reported an increased occurrence of postoperative testicular atrophy associated with delays exceeding 24 hours (\u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e). Later, Tian et al. observed that delaying surgery beyond 12 hours significantly increased testicular volume loss compared to operations performed within 12 hours. Additionally, postponing surgery beyond 24 hours was strongly linked to a higher risk of testicular atrophy (\u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e). However, the exact determination of the time interval between initial symptoms and presentation to the Emergency Department remains challenging. The low accuracy in recalling the exact onset of symptoms, combined with delays in seeking medical assistance by children and adolescents, can distort the actual timeframe from the start of testicular ischemia. Furthermore, not all cases of TT present with acute, intense scrotal pain. Some patients exhibit variable initial symptoms, including discomfort localized to the lower abdomen or inguinal region (\u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e27\u003c/span\u003e).\u003c/p\u003e \u003cp\u003eIn addition to delayed surgery, the degree of torsion has also been recognized as a key prognostic factor for testicular viability during surgical intervention (\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e, \u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e). Several authors have reported a correlation between a higher degree of testicular rotation and an increased likelihood of undergoing orchidectomy compared to orchiopexy (\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e, \u003cspan citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e). While lower degrees of torsion may allow partial restoration of blood flow to the testicle, leading surgeons to opt for testis-salvaging procedures in hopes of preserving function and delaying orchiectomy unless absolutely necessary, many testicles still experience substantial atrophy when monitored over the long term in clinical follow-up (\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e). Chen et al. proposed a nomogram to estimate the likelihood of testicular salvage based on factors such as symptom duration, intratesticular blood flow, degree of spermatic cord torsion, and monocyte count. However, the subjective variability of certain parameters and the limited sample size pose challenges for generalizing these findings (\u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e30\u003c/span\u003e). Howe et al. identified cut-off values for testicular loss at 8.5 hours and 495 degrees of torsion, offering sensitivities of 73% and 53%, respectively, with a specificity of 80% for both thresholds (\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e). Some authors have described other clinical or ultrasound predictors of TA such as red scrotal changes or heterogeneous testis parenchyma on ultrasound, but no objective laboratory parameters such as those included in our study have been analyzed to date (\u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e, \u003cspan citationid=\"CR31\" class=\"CitationRef\"\u003e31\u003c/span\u003e).\u003c/p\u003e \u003cp\u003eWe had previously described the usefulness of NLR in the diagnosis of TT, and we have recently observed that this marker is also useful in diagnosis of ovarian torsion in pediatric population (\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e). However, its usefulness as a prognostic marker had not been explored until now. This study allows us to broaden the application of NLR not only as a diagnostic marker but also as a prognostic marker of TT. The inflammatory response triggered by testicular ischemia results in neutrophilia, driven by chemotaxis and increased release of neutrophils from the bone marrow into peripheral circulation, along with lymphopenia caused by elevated endogenous cortisol levels induced by ischemia (\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e). These combined mechanisms lead to an increase in the NLR through two distinct pathways. This could account for the higher AUC observed for NLR compared to other inflammatory markers like PLR, SIRI, or SII, which involve monocytes or platelets, as NLR reflects the synergistic cellular response of neutrophilia and lymphopenia more directly. This systemic inflammation observed in the laboratory study of patients with suspected TT thus provides prognostic information on the evolution and repercussions of ischemia in the short and medium term, such as orchiectomy or testicular atrophy, and makes it possible to predict them more accurately than clinical or intraoperative data.\u003c/p\u003e \u003cp\u003eThis study included a substantial participant pool, offering significant insights into the enduring outcomes of pediatric patients with TT. In addition, it analyses the predictive role of different clinical parameters (age at torsion, time since symptom onset, degree of twisting cord), together with laboratory data commonly used in clinical practice, all derived from the haemogram. Furthermore, it examines for the first time several inflammatory markers, including NLR, PLR, SIRI, and SII, which can be easily derived from routine blood tests, and to compare these markers with clinical and ultrasound findings, mimicking everyday clinical scenarios. The results highlight NLR as the most accurate and reliable indicator for predicting testicular loss either by orchiectomy or testicular atrophy in male children after testicular torsion. This parameter is more consistent than clinical observations, such as the duration of symptoms, which can be unreliable due to the gradual onset of pain in some instances. Furthermore, NLR eliminates the variability associated with radiological evaluations, such as ultrasound, which can depend heavily on the examiner's expertise. All this facilitates the practical use of the findings on a day-to-day basis, which makes them highly applicable in clinical practice.\u003c/p\u003e \u003cp\u003eHowever, there are several limitations to consider. Firstly, the retrospective design confines the analysis to data already recorded in medical records, potentially introducing recall and selection biases. Additionally, the lack of similar studies in pediatric patients complicates direct comparison of results. Another limitation is the variability in ultrasound imaging, as it was not consistently performed by the same radiologist, which may affect the accuracy of testicular volume calculations, Doppler flow identification or whirlpool sign visualization. Furthermore, the study did not explore the impact of testicular atrophy on hormone production, semen quality and fertility, which added to the relatively short-term follow-up makes that long-term outcomes such as testicular atrophy and infertility may not be fully captured. It is possible that testicular development during puberty could reveal significant volume loss in pre-pubertal boys who experienced testicular torsion. Therefore, caution is advised when generalizing these findings, and further prospective studies with longer follow-up periods are still needed to validate these results.\u003c/p\u003e"},{"header":"Conclusion","content":"\u003cp\u003eThe neutrophil-to-lymphocyte ratio (NLR) can be considered the most reliable predictor of the risk of orchiectomy or testicular atrophy following torsion in pediatric patients, aiding in the identification of high-risk cases. It may prove useful both for obtaining more accurate preoperative information regarding the patient's prognosis and for closer monitoring of patients at elevated risk of testicular atrophy.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003e\u003cu\u003eConflicts of interest\u003c/u\u003e\u003c/strong\u003e: The authors of this manuscript do not refer to any conflict of interest.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cu\u003eFunding\u003c/u\u003e\u003c/strong\u003e: This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cu\u003eEthics approval\u003c/u\u003e\u003c/strong\u003e: The study protocol obtained the approval of Fundación Jiménez Díaz Hospital Institutional Review Board (IRB number PI-264-23) and complied with the guidelines of the Declaration of Helsinki (as revised in 2013).\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n \u003cli\u003eDrl\u0026iacute;k M, Kočvara R. 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Front Pediatr. 2020;8:584796.\u003c/li\u003e\n \u003cli\u003eYu CJ, Zhao J, Luo J, Hong YF, Zhao TX, Wen S, et al. Long-term follow-up results of testicular torsion in children. Asian J Androl. 2022;24(6):653-9.\u003c/li\u003e\n \u003cli\u003eLian BS, Ong CC, Chiang LW, Rai R, Nah SA. Factors predicting testicular atrophy after testicular salvage following torsion. Eur J Pediatr Surg. 2016; 26:17\u0026ndash;21.\u003c/li\u003e\n \u003cli\u003ePogorelić Z, Mrklić I, Jurić I. Do not forget to include testicular torsion in differential diagnosis of lower acute abdominal pain in young males. J Pediatr Urol. 2013;9(6 Pt B):1161-5.\u003c/li\u003e\n \u003cli\u003eBoettcher M, Krebs T, Bergholz R, Wenke K, Aronson D, Reinshagen K. Clinical and sonographic features predict testicular torsion in children: a prospective study. BJU Int. 2013;112:1201-6.\u003c/li\u003e\n \u003cli\u003eFeng S, Yang H, Lou Y, Ru W, Wang A, Liu W. Clinical Characteristics of Testicular Torsion and Identification of Predictors of Testicular Salvage in Children: A Retrospective Study in a Single Institution. Urol Int. 2020;104(11-12):878-83.\u003c/li\u003e\n \u003cli\u003eChen P, Huang W, He Y, Sun M, Sun X, Huang Y, et al. A nomogram for predicting risk factors of testicular salvage after testicular torsion in children. Int J Urol. 2024;31(5):568-74.\u003c/li\u003e\n \u003cli\u003eGrimsby GM, Schlomer BJ, Menon VS, Ostrov L, Keays M, Sheth KR, et al. Prospective Evaluation of Predictors of Testis Atrophy After Surgery for Testis Torsion in Children. Urology. 2018;116:150-155.\u003c/li\u003e\n \u003cli\u003eDelgado-Miguel C, Arredondo-Montero J, Moreno-Alfonso JC, San Basilio M, Pe\u0026ntilde;a P\u0026eacute;rez R, Carrera N, et al. The Role of Neutrophyl-to-Lymphocyte Ratio as a Predictor of Ovarian Torsion in Children: Results of a Multicentric Study. Life (Basel). 2024;14(7):889.\u003c/li\u003e\n\u003c/ol\u003e"},{"header":"Tables","content":"\u003cp\u003eTables 1 to 5 are available in the Supplementary Files section\u003c/p\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Spermatic Cord Torsion, Orchiectomy, Testicular Atrophy, Biomarkers, Pediatrics","lastPublishedDoi":"10.21203/rs.3.rs-5700353/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-5700353/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eIntroduction\u003c/strong\u003e: Neutrophil-to-lymphocyte ratio (NLR) is an inflammatory biomarker (hemogram-derived-ratio) related to ischemic-inflammatory diseases. Its usefulness in the diagnosis of pediatric testicular torsion (TT) has recently been reported, although its prognostic implication has not been evaluated. Our aim is to analyze the role of NLR in the evolution of TT in children, determining its potential for predicting the risk of adverse outcomes such as orchiectomy or testicular atrophy.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethods\u003c/strong\u003e: We performed a retrospective multicentric case-control study in patients with clinical and ultrasound suspicion of TT, in whom surgical testicular examination was performed between 2016-2022 in seven pediatric hospitals. Patient’s outcomes were analyzed according to the intraoperative and postoperative evolution (orchiectomy/testicular atrophy or not). Demographics, clinical, ultrasound and laboratory features at admission were analyzed. Sensitivity and specificity were determined by the area under the curve (AUC) represented on the receiver operating characteristic (ROC) curves.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults\u003c/strong\u003e: A total of 455 patients (median age 13.2 years; interquartile range 10.6-14.4 years) were included, in whom 87 orchiectomies (19.1%) were performed and 34 cases of testicular atrophy (7.5%) were observed during follow-up (median follow-up: 10 months). When comparing clinical, ultrasound and laboratory predictors of both events on ROC curves, NLR was the most sensitive and specific parameter for predicting orchiectomy (AUC=0.834; p\u0026lt;0.001), as well as testicular atrophy (AUC=0.849; p\u0026lt;0.001). Compared with other parameters, the designed cut-off point of NLR=5.2 had maximum sensitivity and specificity (82.2% and 77.0%, respectively) for predicting orchiectomy or atrophy after testicular torsion.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusion\u003c/strong\u003e: NLR may be considered as the best predictor for the risk of orchiectomy or testicular atrophy following torsion in pediatric patients, helping the identification of high-risk cases. It can be useful both for obtaining more accurate preoperative information on patient prognosis and for closer follow-up of high-risk testicular atrophy patients.\u003c/p\u003e","manuscriptTitle":"The Role of Neutrophyl-to-lymphocyte Ratio as a Predictor of Orchiectomy or Testicular Atrophy After Torsion in Children. 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