Severe Acute Respiratory Syndrome Coronavirus-2 antigen and Immunoglobulin G positivity among residents of South-Central Uganda: September 2021 – August 2022

Severe Acute Respiratory Syndrome Coronavirus-2 antigen and Immunoglobulin G positivity among residents of South-Central Uganda: September 2021 – August 2022 | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 22 June 2025 V1 Latest version Share on Severe Acute Respiratory Syndrome Coronavirus-2 antigen and Immunoglobulin G positivity among residents of South-Central Uganda: September 2021 – August 2022 Authors : Charles Ssuuna 0000-0002-2115-5840 [email protected] , Aggrey Anok , Victor Ssempijja , Godfrey Kigozi , Fred Nalugoda , Maria Wawer , Ronald Gray , … Show All … , Larry Chang , Thomas Quinn , Joseph Kagaayi , David Serwadda , Mathew Cotten , Phan , Gertrude Nakigozi , Morgan Otita , Mohammed Lamorde , Stephen Okoboi , Lisa Nelson , Julie Harris , Lisa Mills , Ronald Galiwango , and Steven Reynolds Show Fewer Authors Info & Affiliations https://doi.org/10.22541/au.175059560.07564933/v1 224 views 146 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Background: Insights into SARS-CoV-2 in Uganda were limited throughout the epidemic due to incomplete access to diagnostic testing and limited formal surveillance systems. From September 2021 to August 2022, we conducted a household-based surveillance study using both rapid antigen and serologic testing to determine the SARS-CoV-2 infection burden and seroprevalence among residents of South-Central Uganda. Methods: Household members in four communities within the districts of Kyotera and Lyantonde were recruited. Using a structured questionnaire, participants provided sociodemographic and other information. They also provided nasal swabs and blood samples. Swabs were tested using SD Biosensor Standard Q COVID-19 rapid antigen tests. Sera were tested for antibodies to SARS-CoV-2 using the VivaChek Biotech VivaDiag TM IgM/IgG rapid test. We assessed positivity rates overall and by selected socio-demographic characteristics. Modified Poisson regression was used to assess the relationships between IgG seropositivity and antigen positivity, while accounting for clustering by community. Results: From September 2021 to August 2022, 11,113 participants were enrolled. The mean age was 20.6 (SD = 16.1) years, with an average household size of 3.9 (SD = 2.6) persons. Among 11,086 persons tested for SARS-CoV-2 antigen, overall positivity was 0.61%. Among 9,663 participants tested for SARS-CoV-2 antibodies, 6,630 (69%) were IgG-positive. COVID-19 vaccination coverage was 33.4%, and vaccination was significantly associated with IgG seropositivity (aRR = 0.96; 95% CI: 0.94-0.98; p <0.001). Conclusion : We observed high SARS-CoV-2 antigen rapid testing acceptability, low antigen positivity, and moderate IgG seroprevalence in this setting, suggesting low concurrent transmission but significant prior natural infection. Supplementary Material File (sars-cov-2_surveillance_study_manuscript_16.06.2025.docx) Download 145.58 KB Information & Authors Information Version history V1 Version 1 22 June 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords antigen igg positivity sars-cov-2 south-central uganda Authors Affiliations Charles Ssuuna 0000-0002-2115-5840 [email protected] Rakai Health Sciences Program View all articles by this author Aggrey Anok Rakai Health Sciences Program View all articles by this author Victor Ssempijja National Cancer Institute Frederick National Laboratory for Cancer Research View all articles by this author Godfrey Kigozi Rakai Health Sciences Program View all articles by this author Fred Nalugoda Rakai Health Sciences Program View all articles by this author Maria Wawer Rakai Health Sciences Program View all articles by this author Ronald Gray Rakai Health Sciences Program View all articles by this author Larry Chang Rakai Health Sciences Program View all articles by this author Thomas Quinn Johns Hopkins University Bloomberg School of Public Health View all articles by this author Joseph Kagaayi Rakai Health Sciences Program View all articles by this author David Serwadda Rakai Health Sciences Program View all articles by this author Mathew Cotten MRC/UVRI and LSHTM Uganda Research Unit View all articles by this author Phan MRC/UVRI and LSHTM Uganda Research Unit View all articles by this author Gertrude Nakigozi Rakai Health Sciences Program View all articles by this author Morgan Otita Makerere University Infectious Diseases Institute View all articles by this author Mohammed Lamorde Makerere University Infectious Diseases Institute View all articles by this author Stephen Okoboi Makerere University Infectious Diseases Institute View all articles by this author Lisa Nelson Centers for Disease Control and Prevention Global Health Uganda View all articles by this author Julie Harris Centers for Disease Control and Prevention Global Health Uganda View all articles by this author Lisa Mills Centers for Disease Control and Prevention Global Health Uganda View all articles by this author Ronald Galiwango Rakai Health Sciences Program View all articles by this author Steven Reynolds Johns Hopkins University Center for Global Health View all articles by this author Metrics & Citations Metrics Article Usage 224 views 146 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Charles Ssuuna, Aggrey Anok, Victor Ssempijja, et al. Severe Acute Respiratory Syndrome Coronavirus-2 antigen and Immunoglobulin G positivity among residents of South-Central Uganda: September 2021 – August 2022. Authorea . 22 June 2025. DOI: https://doi.org/10.22541/au.175059560.07564933/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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