Thrombospondin Encapsulated Dense Particles accelerate wound healing and downregulate fibrotic phenotypes

Thrombospondin Encapsulated Dense Particles accelerate wound healing and downregulate fibrotic phenotypes | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Thrombospondin Encapsulated Dense Particles accelerate wound healing and downregulate fibrotic phenotypes Michael Dustin, Claire Staton, Marcus Widdess, Ashwin Jainarayanan, and 12 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7943553/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Extracellular particles (EP) released by cells are heterogenous, with extracellular vesicles (EV) believed to be the major component. To investigate different EV subpopulations, we conducted a panel screen of 15 fluorescent EV markers in 4 adherent cell lines. Nanoflow cytometry identifies TSPAN14 as the best EV marker in 3 of 4 cell lines. Surprisingly, less than 50% of EPs are labelled by the top 2 EV markers in half the cell lines. The EV marker negative EPs are detergent resistant, non-lipophilic, dense and have Thrombospondin shells by dSTORM; and were thus named Thrombospondin Encapsulated Dense particles (ThrEDs). ThrEDs are ubiquitous, contain extracellular matrix regulators and are released following cell stress. ThrEDs accelerate scratch-wound closure and downregulate the fibrotic phenotype in Dupuytren’s disease patient myofibroblasts. The TSP-1 C-terminus is exposed on the surface of ThrEDs, opening engineering applications. Biological sciences/Cell biology/Cell signalling/Extracellular signalling molecules Biological sciences/Drug discovery/Biologics/Cell therapies Health sciences/Diseases/Immunological disorders/Inflammatory diseases Biological sciences/Cell biology/Mechanisms of disease Biological sciences/Cell biology/Cell adhesion/Extracellular matrix ThrED SMAP NVEP subpopulation proteinaceous particle shell delivery wound healing fibrosis Full Text Additional Declarations Yes there is potential Competing Interest. M.L. Dustin and C.C. Staton have filed a provisional patent on proteinaceous particles: ThrEDs isolation and engineering. Supplementary Files StatonetalThrEDsNCBsupp.pdf Supplemental Figures and legends Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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