Evaluation of oxidative stress biomarkers in HPV-positive women undergoing cancer prevention in the Brazilian Amazon: a pilot study

Evaluation of oxidative stress biomarkers in HPV-positive women undergoing cancer prevention in the Brazilian Amazon: a pilot study | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 25 June 2025 V1 Latest version Share on Evaluation of oxidative stress biomarkers in HPV-positive women undergoing cancer prevention in the Brazilian Amazon: a pilot study Authors : Gleyce de Fátima Santos Rotterdam , Sinara Mendes Campelo , Rogério Valois Laurentino 0000-0001-6986-1309 , Saul Rassy Carneiro , Rosilma dos Santos Albuquerque , Ericka Bechara Ferreira , Elza Baia de Brito , … Show All … , Fabiana Costa Cardoso , Luis Fernando Almeida Machado , Maísa Silva de Sousa , Karla Valéria Batista Lima , Leonardo Miranda dos Santos 0000-0003-0253-7277 [email protected] , and Maria Conceição Nascimento Pinheiro Show Fewer Authors Info & Affiliations https://doi.org/10.22541/au.175083489.96133330/v1 199 views 123 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Introduction: Cervical cancer is a public health problem in resource-poor settings, such as the Brazilian Amazon. HPV infection is the leading cause of cervical cancer. Oxidative stress is an adjuvant in HPV carcinogenesis. Objectives: To assess the prevalence of HPV and its association with oxidative stress biomarkers among women from Belém, Pará. Methods: A cross-sectional pilot study was conducted with 71 sexually active women from Belém, Pará, between March 2021 and March 2022. Sociodemographic, epidemiological, and reproductive health data were collected. Endocervical samples were analyzed for HPV DNA using PCR, and oxidative stress biomarkers malondialdehyde (MDA) and glutathione (GSH) were measured in blood samples. Statistical analyses were performed using SPSS version 21.0. Results: The prevalence of HPV infection was 28.2% (20/71). Participants aged 39–48 years had significantly higher MDA levels (≥3.54 nmol/mL), with 34% exhibiting elevated levels (p-value = 0.036). No significant differences in GSH levels were observed between HPV-positive and HPV-negative women, although HPV-positive women tended to have lower GSH levels (p-value = 0.090). Most HPV-positive women had low income, limited education, and cytologic atypia/inflammation. Discussion: These findings suggest that oxidative stress may play a role in HPV persistence and cervical carcinogenesis, warranting further investigation. Conclusions: This study highlights the high prevalence of HPV infection and oxidative stress among women in the Brazilian Amazon. Biomarkers of oxidative stress (MDA and GSH) are valuable and cost-effective options. Supplementary Material File (manuscript.docx) Download 70.66 KB Information & Authors Information Version history V1 Version 1 25 June 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Authors Affiliations Gleyce de Fátima Santos Rotterdam Universidade Federal do Para View all articles by this author Sinara Mendes Campelo Universidade Federal do Para View all articles by this author Rogério Valois Laurentino 0000-0001-6986-1309 Universidade Federal do Para View all articles by this author Saul Rassy Carneiro Universidade Federal do Para View all articles by this author Rosilma dos Santos Albuquerque Universidade Federal do Para View all articles by this author Ericka Bechara Ferreira Universidade Federal do Para View all articles by this author Elza Baia de Brito Universidade Federal do Para View all articles by this author Fabiana Costa Cardoso Universidade Federal do Para View all articles by this author Luis Fernando Almeida Machado Universidade Federal do Para View all articles by this author Maísa Silva de Sousa Universidade Federal do Para View all articles by this author Karla Valéria Batista Lima Universidade Federal do Para View all articles by this author Leonardo Miranda dos Santos 0000-0003-0253-7277 [email protected] Universidade Federal do Para View all articles by this author Maria Conceição Nascimento Pinheiro Universidade Federal do Para View all articles by this author Metrics & Citations Metrics Article Usage 199 views 123 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Gleyce de Fátima Santos Rotterdam, Sinara Mendes Campelo, Rogério Valois Laurentino, et al. Evaluation of oxidative stress biomarkers in HPV-positive women undergoing cancer prevention in the Brazilian Amazon: a pilot study. Authorea . 25 June 2025. DOI: https://doi.org/10.22541/au.175083489.96133330/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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