Turmeric Phyto-nanoparticle: enhanced cellular bioavailability and anti-inflammatory effect in human monocyte / macrophage model

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The paper investigates whether an all-natural turmeric-derived “Turmeric Phyto-Nanoparticle” (TPNP) formulation can overcome the poor bioavailability of curcuminoids by enhancing antioxidant and anti-inflammatory activity in a human monocyte/macrophage cell model. Using a TPNP formulation enriched with curcuminoids (reported as 24.85% by mass) and compared against conventionally purified curcuminoids, the authors find improved cellular uptake and higher antioxidant capacity, alongside a cellular pharmacodynamic anti-inflammatory effect characterized by increased heme oxygenase-1 (HMOX1) and greater suppression of LPS-induced TNF secretion. A stated limitation is the work is confined to an in vitro human monocyte/macrophage model assessing cellular kinetics and effects rather than in vivo outcomes or clinical efficacy. Relevance to endometriosis: the study does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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Abstract

The poor bioavailability of curcuminoids remains a major challenge to therapeutic use. This is largely due to their hydrophobicity, poor absorption, rapid metabolism, and short circulating half-life—limitations that are now being addressed through advances in nano- and micro-emulsion technologies. Curcuminoids and other water-insoluble phyto-polyphenols offer significant putative health benefits as anti-inflammatory, antioxidant, anticancer, radioprotective, and neuroprotective agents. Conventional emulsion-based delivery systems, such as liposomes, micelles, or solid lipid particles, rely on various emulsifying surfactants and/or excipients, some of which may themselves pose health risks. Here, we establish a novel class of all-natural, additive-free, oil-free, and emulsion-free Turmeric Phyto-NanoParticles (TPNPs) formulated directly from turmeric rhizomes and tested in a human monocyte/macrophage cell model to assess bioavailability kinetics and the efficacy of antioxidant and anti-inflammatory potential. TPNPs are enriched with curcuminoids (24.85% by mass), form a homogeneous nanoparticle distribution, exhibit higher antioxidant capacity, and demonstrate significantly improved cellular uptake in both monocytes and macrophages compared to conventionally purified curcuminoids. Favourable cellular pharmacodynamic anti-inflammatory effect of TPNPs was shown by increased levels of the cytoprotective enzyme heme oxygenase-1 (HMOX1), and a more effective reduction in lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF) secretion compared to conventional curcuminoids. TPNPs could thus serve as a stable, non-synthetic, excipient-free formulation for safe and effective delivery of curcuminoids by nanocarriers for inflammatory conditions.
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Abstract The poor bioavailability of curcuminoids remains a major challenge to therapeutic use. This is largely due to their hydrophobicity, poor absorption, rapid metabolism, and short circulating half-life—limitations that are now being addressed through advances in nano- and micro-emulsion technologies. Curcuminoids and other water-insoluble phyto-polyphenols offer significant putative health benefits as anti-inflammatory, antioxidant, anticancer, radioprotective, and neuroprotective agents. Conventional emulsion-based delivery systems, such as liposomes, micelles, or solid lipid particles, rely on various emulsifying surfactants and/or excipients, some of which may themselves pose health risks. Here, we establish a novel class of all-natural, additive-free, oil-free, and emulsion-free Turmeric Phyto-NanoParticles (TPNPs) formulated directly from turmeric rhizomes and tested in a human monocyte/macrophage cell model to assess bioavailability kinetics and the efficacy of antioxidant and anti-inflammatory potential. TPNPs are enriched with curcuminoids (24.85% by mass), form a homogeneous nanoparticle distribution, exhibit higher antioxidant capacity, and demonstrate significantly improved cellular uptake in both monocytes and macrophages compared to conventionally purified curcuminoids. Favourable cellular pharmacodynamic anti-inflammatory effect of TPNPs was shown by increased levels of the cytoprotective enzyme heme oxygenase-1 (HMOX1), and a more effective reduction in lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF) secretion compared to conventional curcuminoids. TPNPs could thus serve as a stable, non-synthetic, excipient-free formulation for safe and effective delivery of curcuminoids by nanocarriers for inflammatory conditions. Competing Interest Statement The authors have declared no competing interest. Footnotes To reorganize some text, expand the discussion.

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