Metabolic Brain Imaging with Glucosamine CEST MRI: In Vivo Characterization and First Insights

Metabolic Brain Imaging with Glucosamine CEST MRI: In Vivo Characterization and First Insights | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Metabolic Brain Imaging with Glucosamine CEST MRI: In Vivo Characterization and First Insights Michal Rivlin, Or Perlman, Gil Navon This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3368953/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 12 Dec, 2023 Read the published version in Scientific Reports → Version 1 posted 8 You are reading this latest preprint version Abstract The utility of chemical exchange saturation transfer (CEST) MRI for monitoring the uptake of glucosamine (GlcN), a safe dietary supplement, has been previously demonstrated in detecting breast cancer in both murine and human subjects. Here, we studied and characterized the detectability of GlcN uptake and metabolism in the brain. Following intravenous GlcN administration in mice, CEST brain signals calculated by magnetization transfer ratio asymmetry (MTRasym) analysis, were significantly elevated, mainly in the cortex, hippocampus, and thalamus. The in vivo contrast remained stable during 40 minutes of examination, which can be attributed to GlcN uptake and its metabolic products accumulation as confirmed using 13 C NMR spectroscopic studies of brain extracts. A Lorentzian multi-pool fitting analysis revealed an increase in the hydroxyl, amide, and relayed nuclear Overhauser effect (rNOE) signal components after GlcN treatment. With its ability to cross the blood-brain barrier (BBB), the GlcN CEST technique has the potential to serve as a metabolic biomarker for the diagnosis and monitoring various brain disorders. Health sciences/Biomarkers Physical sciences/Chemistry Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 12 Dec, 2023 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Major revision 17 Oct, 2023 Reviews received at journal 06 Oct, 2023 Reviewers agreed at journal 20 Sep, 2023 Reviewers invited by journal 20 Sep, 2023 Editor assigned by journal 20 Sep, 2023 Editor invited by journal 20 Sep, 2023 Submission checks completed at journal 20 Sep, 2023 First submitted to journal 19 Sep, 2023 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3368953","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":234963897,"identity":"75ad7494-4895-479a-8a93-40522b4e3c14","order_by":0,"name":"Michal Rivlin","email":"","orcid":"","institution":"Tel Aviv University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Michal","middleName":"","lastName":"Rivlin","suffix":""},{"id":234963899,"identity":"5a32d4dc-016f-4d9e-a127-49a7416f63c5","order_by":1,"name":"Or Perlman","email":"","orcid":"","institution":"Tel Aviv University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Or","middleName":"","lastName":"Perlman","suffix":""},{"id":234963900,"identity":"d619a3ca-dbb1-400a-9d6b-734c3fbd4d20","order_by":2,"name":"Gil Navon","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA4UlEQVRIiWNgGAWjYNCCCjT+gQcEtZwxgCqFaUkgpIOxDU0LAz4t5uxnDB8Xzvsjb3C8x4D5Q81hBv72A4x4bbHsyTE2nrnNwHDDmWMJDAeOHWaQOJOA32EGB9LSpHm3GTBuuJF8gOEA22EGhhsE/GJw/hlQyxwD+w33HzYwHPh3mEGeoJYbycekeRsMEjfcYD7AcLDtMFCEoJbHh415jhknzzyTlnDgbF86j+GZxAYCDktsfMxTI2fbd/yM4YOKb9ZycscPH/7wAY8WFHAAiHmA0dRArIZRMApGwSgYBTgAAM0vVCKY3IBMAAAAAElFTkSuQmCC","orcid":"","institution":"Tel Aviv University","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Gil","middleName":"","lastName":"Navon","suffix":""}],"badges":[],"createdAt":"2023-09-19 10:29:13","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-3368953/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-3368953/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1038/s41598-023-48515-5","type":"published","date":"2023-12-12T15:01:51+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":48402043,"identity":"2a7eff14-7b67-4fe6-adb4-4b802b345eab","added_by":"auto","created_at":"2023-12-18 15:12:23","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":703260,"visible":true,"origin":"","legend":"","description":"","filename":"MetabolicBrainImagingwithGlcN.pdf","url":"https://assets-eu.researchsquare.com/files/rs-3368953/v1_covered_43f38217-000d-44d3-b9e4-bd1da804fc25.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Metabolic Brain Imaging with Glucosamine CEST MRI: In Vivo Characterization and First Insights","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-3368953/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3368953/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThe utility of chemical exchange saturation transfer (CEST) MRI for monitoring the uptake of glucosamine (GlcN), a safe dietary supplement, has been previously demonstrated in detecting breast cancer in both murine and human subjects. 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