Graphene Oxide Induced Oxidative Stress Gene Expression and Gut Microbiome Profiling in in Vivo Drosophila Melanogaster Model

In the present study we have attempted to reveal the induction of stress, changes in behavioral, molecular, and gut microbiome profile by Graphene oxide (GO) in Drosophila melanogaster . The study revealed that GO does not exert accountable mortality in larvae but had little impact on the formation of pupa from larvae in the treatment group compared to the control group. Similarly, the larval movement exhibited little change in the treatment group (21) compared to the control group (17). The flies that emerged from with and without GO treatment were analyzed for the expression of Glutathione S transferase D 1 ( Gstd1 ) and Acetylcholine Esterase ( AchE ) gene. In the gut-microbiome study, GO administration had metabolic change and was well measured with increased Acetobacteraceae to survive in the stress-induced conditions. Hence it was concluded that GO was not lethal at 300µg/mL and hence its application in the drug delivery tool or carrier perhaps cause are. Instead, it can able to induce oxidative stress and changes in the regulation of the gene attributed to oxidative stress and locomotor behavior.