Phytochemical profiling, antioxidant and nephroprotective activities of Ziziphus lotus Lam. leaf extract in paracetamol-induced renal toxicity in rats and molecular docking analysis

Phytochemical profiling, antioxidant and nephroprotective activities of Ziziphus lotus Lam. leaf extract in paracetamol-induced renal toxicity in rats and molecular docking analysis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Phytochemical profiling, antioxidant and nephroprotective activities of Ziziphus lotus Lam. leaf extract in paracetamol-induced renal toxicity in rats and molecular docking analysis Cletus Anes Ukwubile, Nnamdi David Menkiti, Ahamefula Anslem Ahuchaogu, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4637155/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Ziziphus lotus Lam. is a medicinal plant that is used mostly in Nigeria and parts of Africa for treating many diseases. The study was aimed at assessing the phytoconstituents, evaluating antioxidant and nephroprotective activities against paracetamol-induced renal toxicity in rats and molecular docking analysis of leaf extract. Liquid-liquid partitioning was carried out for most active fraction while column chromatography was used for the isolation of bioactive compounds and analyzed by the GC-MS. Total phenolic and total flavonoid contents were determined using standard methods, antioxidant activity by DPPH (2-2-diphenyl-1-picrylhydrazyl) and ABTS (2,2’azinobis (3ethyl-benzothiazoline-6sulfonic acid) scavenging assays and nephroprotective activity was evaluated at various extract doses in rats. Molecular docking studies were carried out using AutoDock Vina software. The GC-MS profiling of the extract revealed the presence of 18 compounds with five compounds showing the strongest activities. Molecular docking studies of the most bioactive compounds predicted potential antioxidant and nephroprotective effects. The results further revealed a total phenolic content of 408.12 mg GAE/g and a flavonoid content of 88.01 mg QE/g. It also showed significant (p < 0.05) antioxidant activities against DPPH and ABTS radical scavenging at concentrations 0 to 800 µg/mL. There were significant abnormal increases in the biochemical parameters before the treatment of rats, and these increases were reduced significantly on administration of the leaf extract. Our study showed that Z. lotus extract possessed antioxidant activity and reversed kidney toxicity, thus, justifying its uses as an ethnomedicinal remedy for kidney problems. Ziziphus lotus antioxidant paracetamol nephrotoxicity biochemical parameters molecular docking Full Text Additional Declarations No competing interests reported. Supplementary Files SUPPLEMENTRAYFILEZIZIPHUS.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4637155","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":331163047,"identity":"a2588bf4-fe48-4611-9adf-22051d40e1eb","order_by":0,"name":"Cletus Anes 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The study was aimed at assessing the phytoconstituents, evaluating antioxidant and nephroprotective activities against paracetamol-induced renal toxicity in rats and molecular docking analysis of leaf extract. Liquid-liquid partitioning was carried out for most active fraction while column chromatography was used for the isolation of bioactive compounds and analyzed by the GC-MS. Total phenolic and total flavonoid contents were determined using standard methods, antioxidant activity by DPPH (2-2-diphenyl-1-picrylhydrazyl) and ABTS (2,2\u0026rsquo;azinobis (3ethyl-benzothiazoline-6sulfonic acid) scavenging assays and nephroprotective activity was evaluated at various extract doses in rats. Molecular docking studies were carried out using AutoDock Vina software. The GC-MS profiling of the extract revealed the presence of 18 compounds with five compounds showing the strongest activities. 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