Biomedical Applications Of Novel Green Biomaterial Synthesized From Endophytic Bacteria Cronobacter Sakazakii

Abstract Endophytic bacteria from the flower of Nyctanthes arbor-tristis was isolated and identified as Cronobacter sakazakii. The supernatant of the biomass of this endophyte was utilized for biomaterial synthesis which was confirmed by a change in colour and characterized by using UV-Visible spectrophotometer, FTIR, FESEM, EDAX, Zeta potential and DLS. The antibacterial efficacy of the biomaterial against Gram positive and Gram negative bacteria was determined via agar well diffusion, MIC, MBC and biofilm formation assays. The antioxidant and anti-arthritic assays were also performed. The result reveals antibacterial effect of biomaterial against the multi-drug-resistant E. coli, S. aureus and K. pneumonia strains. This report is the first to reveal the isolation and identification of endophytic bacteria from the flower of Nyctanthes arbor-tristis, alongside its biomaterial synthesis, characterization and biomedical applications. Our result implies that the synthesized biomaterial can be utilized as an effective antimicrobial, antioxidant and anti-arthritic agent and can be incorporated in pharmaceutical formulation for drug development.