The oxidative cost of reproduction in female and male Semaprochilodus taeniurus

The oxidative cost of reproduction in female and male Semaprochilodus taeniurus | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article The oxidative cost of reproduction in female and male Semaprochilodus taeniurus Rosa Karina Solis Garcia, Jhonatan Mota da Silva, Adalberto Luis Val This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5587875/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Long migrations are carried out by animals for various ecological purposes, with reproduction being one of the most important. During this process, the energy demand is increased, requiring adjustments at the physiological level to deal with high energy consumption and the consequent associated oxidative stress. To test the oxidative stress as a physiological cost of reproduction, hematological parameters, antioxidant enzyme and oxidative damage in the heart and liver of female and male Semaprochilodus taeniurus were measured at the end of the reproductive migration after the animals were collected in the Negro River, near the Lake Catalão, Amazon basin. The following haematological parameters were analysed: hematocrit, haemoglobin concentration, number of erythrocytes, corpuscular constants (MCV, HCM, CHCM), plasma glucose and lactate levels. Oxidative stress was indirectly quantified via measurements of the antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase and glutathione S-transferase) and directly quantified by measuring markers of oxidative damage using the comet assay, which indicates levels of damage to the DNA molecule, and lipid peroxidation, which indicates damage to lipids of cell membranes. The results showed higher values of mean corpuscular hemoglobin (HCM), glucose, glutathione S-transferase, superoxide dismutase, lipid peroxidation and DNA damage in males compared to females. The number of erythrocytes was high in females. Based on the results, we concluded that oxidative stress was higher in males than in females, suggesting that reproductive migration results in a higher physiological cost for males than for females of this species. reactive oxygen species antioxidant enzymes oxidative stress reproductive phase migratory fish Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5587875","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":431125237,"identity":"870ea0e0-cddb-41f6-a77a-09f5a8dbba06","order_by":0,"name":"Rosa Karina Solis Garcia","email":"","orcid":"","institution":"INPA: Instituto Nacional de Pesquisas da Amazonia","correspondingAuthor":false,"prefix":"","firstName":"Rosa","middleName":"Karina Solis","lastName":"Garcia","suffix":""},{"id":431125238,"identity":"0b6e6c43-f6e2-47a0-8457-63818716f835","order_by":1,"name":"Jhonatan Mota da Silva","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABDElEQVRIiWNgGAWjYBACxhkovAYJBn4QI6GAkJYEqAaQFskGkIABHmskULUwMBgcALHxaGGe3Xzswc8fdnkMYoePP/i5w0LO+PzqxA8PDBjk+cUOYHfYnGPphj0JycUM0mmJjb1nJIzNbrzdLAF0mOHM2Qk4/JJjJsGTwJzYIJ1j2MDbJpG47cbZDSAtCQa3cWuR/JNQD9bS+BeoZfOMs5t/ENIizZNwGKylGWTLBv7ebQRsSUuTlkk7ntgG9MtsWaBfJG7wbrNIMJDA6RfDGcnHJN/YVCf2Sycf+Ph2R50cf//ZzTd/VNjI80vj0NIAZbDBhSTAKiWwKgcBeUwh/gM4VY+CUTAKRsHIBABM0F/2qdcs8QAAAABJRU5ErkJggg==","orcid":"https://orcid.org/0000-0001-6907-0507","institution":"INPA: Instituto Nacional de Pesquisas da Amazonia","correspondingAuthor":true,"prefix":"","firstName":"Jhonatan","middleName":"Mota da","lastName":"Silva","suffix":""},{"id":431125239,"identity":"69d1f0f6-2c64-4ed9-9032-ef81f4bb5983","order_by":2,"name":"Adalberto Luis Val","email":"","orcid":"","institution":"INPA: Instituto Nacional de Pesquisas da Amazonia","correspondingAuthor":false,"prefix":"","firstName":"Adalberto","middleName":"Luis","lastName":"Val","suffix":""}],"badges":[],"createdAt":"2024-12-05 14:49:26","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false},"doi":"10.21203/rs.3.rs-5587875/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-5587875/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":80332956,"identity":"9ce4f424-e7c1-4bd8-a608-4302140ea2d3","added_by":"auto","created_at":"2025-04-10 15:44:24","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":788352,"visible":true,"origin":"","legend":"","description":"","filename":"Karinaartigooficial11.pdf","url":"https://assets-eu.researchsquare.com/files/rs-5587875/v1_covered_be09a978-e2fb-4431-82c5-6cc7455e276d.pdf"}],"financialInterests":"","formattedTitle":"The oxidative cost of reproduction in female and male Semaprochilodus taeniurus","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":true,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"reactive oxygen species, antioxidant enzymes, oxidative stress, reproductive phase, migratory fish","lastPublishedDoi":"10.21203/rs.3.rs-5587875/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-5587875/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Long migrations are carried out by animals for various ecological purposes, with reproduction being one of the most important. During this process, the energy demand is increased, requiring adjustments at the physiological level to deal with high energy consumption and the consequent associated oxidative stress. To test the oxidative stress as a physiological cost of reproduction, hematological parameters, antioxidant enzyme and oxidative damage in the heart and liver of female and male Semaprochilodus taeniurus were measured at the end of the reproductive migration after the animals were collected in the Negro River, near the Lake Catalão, Amazon basin. The following haematological parameters were analysed: hematocrit, haemoglobin concentration, number of erythrocytes, corpuscular constants (MCV, HCM, CHCM), plasma glucose and lactate levels. 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