Characterisation of cuticle mechanical properties: analysing stiffness in layered living systems to understand surface buckling patterns

ABSTRACT Development of a living organism is a highly regulated process during which biological materials undergo constant change. De novo material synthesis and changes in mechanical properties of materials are key for organ development; however, few studies have attempted to produce quantitative measurements of the mechanical properties of biological materials during growth. Such quantitative analysis is particularly challenging where the material is layered, as is the case for the plant cuticle on top of the plant epidermal cell wall. Here, we focus on Hibiscus trionum flower petals, where buckling of the cuticle forms ridges, producing an iridescent effect. This ridge formation is hypothesised to be due to mechanical instability, which directly depends upon the mechanical properties of the individual layers within the epidermal cells. We present measurements of the mechanical properties of the surface layers of petal epidermal cells through atomic force microscopy (AFM) and the uniaxial tensile tester for ultrathin films (TUTTUT), across growth stages. We found that the wavelength of the surface ridges was set at the ridge formation stage, and this wavelength was preserved during further petal development, most likely because of the plasticity of the material. Our findings suggest that temporal changes in biological material properties are key to understanding the development of biological surface patterns.