Lysinibacillus Agricola sp. nov.Isolated from Soil

Abstract A Gram-staining-positive, rod-shaped cells, designed strain FJAT-51161T, was isolated from farmland soil collected from Fujian Province, China. Growth was observed at 25 - 40 °C (optimum 30 °C), pH 7.0-9.0 (optimum 7.0), and NaCl tolerance in the range of 0-7 % (w/v), respectively. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain FJAT-51161T belonged to the genus Lysinibacillus, and had the closest relationship with Lysinibacillus xylanilyticus XDB9T (with 99.0 % 16S rRNA sequence similarity). The digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values based on the genome sequence analysis between strain FJAT-51161T and the closest reference strain were 38.0 % for dDDH and 88.7 % for ANI, respectively, lower than the prokaryotic species defined values. Further analysis showed that strain FJAT-51161T shared the fatty acid profiles such as iso-C15:0 (46.7%), iso-C16:0 (15.8%), C16:1 ω7c alcohol (14.0%), anteiso-C15:0 (6.9%) with other members of the genus Lysinibacillus. The peptidoglycan was L-Lys–D-Asp (type A4α). The main quinone was MK-7 and MK-6. The major polar lipids were diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE). The DNA G+C content is 36.6 mol%. Based on the phenotypic characters and taxono-genomics study, strain FJAT-51161T is considered to represent a novel Lysinibacillus species, for which the name Lysinibacillus agricola sp. nov. is proposed. The type strain is FJAT-51161T (= GDMCC1.2350T = KCTC XXXXXXT).