Production of antibodies by perfusion culture of CHO cells

Chinese hamster ovary cells (CHO) are the main cell line used for mass production of recombinant therapeutic proteins (RTPs), especially recombinant therapeutic antibodies (RTAs). In order to increase the yield and quality of RTPs produced by CHO cells, it is essential to optimize the production process and scale of CHO cells. Perfusion culture is an important process for mammalian cell culture that has emerged in recent years. It offers several advantages, including increased levels of protein expression, reduced risk of bacterial infection, simplified downstream processing, lower production costs, support for industrial production, and improved understanding of cell metabolism. It can increase the yield of antibodies and improve four key quality attributes. This review introduces the culture process of CHO cell expressed antibody and the effect of perfusion culture process on the yield and quality of antibody expression.