JA signaling through ZmZIM12 protein to determine nitrogen use efficiency via regulation of nitrate uptake in maize

JA signaling through ZmZIM12 protein to determine nitrogen use efficiency via regulation of nitrate uptake in maize | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article JA signaling through ZmZIM12 protein to determine nitrogen use efficiency via regulation of nitrate uptake in maize Xilei Wang, Qiuxia Li, Shihao Lv, Jiachang Zhang, Jifeng Liu, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8135841/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract JA signaling through ZmZIM12 protein to regulate nitrate uptake, and overexpression of ZmZIM12 protein can improve nitrogen use efficiency in maize. Abstract Nitrogen (N) is an essential nutrient for maize growth and development, and related to maize grain yield. Jasmonic acid (JA) plays an essential role in regulating stress-response and plant growth. However, the regulatory mechanism about JA signaling to regulate nitrate uptake for improving NUE (nitrogen use efficiency) in maize remains elusive. Here, we report that exogenous JA have double effective on absorption nitrate in maize, which low content of JA promotes nitrate uptake, by contrast, high content of JA inhibits nitrate uptake. Jas-ZIM-domain transcriptional repressor 12 (ZmZIM12) acts as an upstreaming transcriptional-repression factor in JA signaling. JA signaling through ZmZIM12 to control root growth and regulate the expression of ZmNRTs for modulating nitrate uptake. In hydroponic, overexpression of ZmZIM12 proteins can promote root growth and increase the expression of ZmNRTs for absorption of nitrate. In two-year field trials, ZmZIM12OE lines has bigger dry matter, higher NUE and more grain yield than WT. Therefore, our data reshape a regulatory mechanism of JA signaling through ZmZIM12 protein to regulate ZmNRTs gene’s expression for mediating nitrate uptake, and provide guidance for improving maize NUE in maize breeding. Full Text Supplementary Files SupplyJAregulatesnitrateuptakeinmaize.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8135841","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":550326176,"identity":"a0750bd2-e2e3-46d2-a8a0-167f85da6cae","order_by":0,"name":"Xilei Wang","email":"","orcid":"","institution":"China Agricultural University","correspondingAuthor":false,"prefix":"","firstName":"Xilei","middleName":"","lastName":"Wang","suffix":""},{"id":550326177,"identity":"14179cff-d2ec-46eb-846a-2c2b4b29387a","order_by":1,"name":"Qiuxia Li","email":"","orcid":"","institution":"China Agricultural 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