An Experimental and Multiphysics Simulations Study of Clostridium carboxidivorans sp. 624 for Acid and Alcohol Production in CO2/H2

2025 · doi:10.1101/2025.02.24.639814
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The study used combined experimental optimization and computational modelling to investigate fermentation dynamics of Clostridium carboxidivorans sp. 624 for producing C2–C6 acids from CO2/H2, assessing how temperature and medium conditions affect metabolic pathway regulation over time. Experiments coupled with modelling identified a gas-liquid volumetric ratio VL/VG = 4 as optimal for maximizing longer-chain acids, which the authors attribute to improved gas solubility and substrate bioavailability. A hybrid dynamic-kinetic plus CFD model predicted product formation and, by representing spatial inhomogeneities in gas-liquid interactions, provided mechanistic insight into process design parameters. This paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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Abstract

Biotechnological advances in CO 2 utilization through gas fermentation offer a sustainable alternative to energy-intensive chemical processes. This study investigates and optimizes fermentation dynamics of Clostridium carboxidivorans sp. 624 for enhanced production of C 2 –C 6 acids via combined experimental and computational methods. A systematic experimental evaluation of temperature and medium conditions, along with time-course analysis elucidates metabolic pathway regulation. Experimental optimisation, complemented by modelling, identified gas-liquid volumetric ratios V L /V G = 4 as optimal for maximizing longer-chain acids production, attributed to enhanced gas solubility and substrate bioavailability. Additionally, a hybrid model combining dynamic-kinetic model and computational fluid dynamics (CFD) successfully predict product formation. By capturing spatial inhomogeneities in gas-liquid interactions, the model also provides critical insights for optimizing fermentation performance and establishes a framework for improving process design parameters for CO 2 /H 2 fermentation, paving the way for scalable and efficient CO 2 -based bioprocesses.
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Abstract

Biotechnological advances in CO2 utilization through gas fermentation offer a sustainable alternative to energy-intensive chemical processes. This study investigates and optimizes fermentation dynamics of Clostridium carboxidivorans sp. 624 for enhanced production of C2–C6 acids via combined experimental and computational methods. A systematic experimental evaluation of temperature and medium conditions, along with time-course analysis elucidates metabolic pathway regulation. Experimental optimisation, complemented by modelling, identified gas-liquid volumetric ratios VL/VG = 4 as optimal for maximizing longer-chain acids production, attributed to enhanced gas solubility and substrate bioavailability. Additionally, a hybrid model combining dynamic-kinetic model and computational fluid dynamics (CFD) successfully predict product formation. By capturing spatial inhomogeneities in gas-liquid interactions, the model also provides critical insights for optimizing fermentation performance and establishes a framework for improving process design parameters for CO2/H2 fermentation, paving the way for scalable and efficient CO2-based bioprocesses. Competing Interest Statement The authors have declared no competing interest. Footnotes

Abstract

updated, manuscript updated, supplemental files updated.

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