Clinical Insights of Honeybee - Propolis Extract: Identification of Biochemical Compound using GC-MS and FTIR Analysis

Abstract Propolis, a natural resinous substance collected by honeybees from various plant sources, has garnered significant interest for its diverse pharmacological properties. This study provides a comprehensive analysis of propolis, focusing on its extraction, biochemical characterization, and detailed identification of its bioactive compounds using Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR). The optimized extraction techniques to identify propolis compounds were followed by a thorough biochemical analysis to identify key constituents. GC-MS analysis revealed a complex mixture of phenolic acids, flavonoids, and terpenes, which are known for their antimicrobial, anti-inflammatory, and antioxidant activities. FTIR spectroscopy complemented these findings by providing detailed insights into the functional groups present in the propolis extract. The study underscores the potential of propolis in clinical applications, highlighting its rich biochemical profile that can be harnessed for therapeutic purposes. The integration of GC-MS and FTIR analyses offers a robust framework for the precise identification and characterization of propolis compounds, paving the way for future research and development in medical and pharmaceutical fields.