Abstract
DNA information storage provides an excellent solution for metadata storage due to its high density, programmability, and long-term stability. However, current research in DNA storage primarily focuses on the processes of storing and reading data, lacking comprehensive solutions for the secure metadata wiping. Herein, we present a method of random sanitization in DNA information storage using CRISPR-Cas12a (RSDISC) based on precise control of the thermodynamic energy of primer-template hybridization. We utilize the collateral cleavage (trans-activity) of single-stranded DNA (ssDNA) by CRISPR-Cas12a to achieve selective sanitization of files in metadata. This method enables ssDNA degradation with different GC content, lengths, and secondary structures to achieve a sanitization efficiency up to 99.9% for 28,258 oligonucleotides in DNA storage within one round. We demonstrate that the number of erasable files could reach 10 11.7 based on a model of primer-template hybridization efficiency. Overall, RSDISC provides a random sanitization approach to set the foundation of information encryption, file classification, memory deallocation and accurate reading in DNA data storage.
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Abstract
DNA information storage provides an excellent solution for metadata storage due to its high density, programmability, and long-term stability. However, current research in DNA storage primarily focuses on the processes of storing and reading data, lacking comprehensive solutions for the secure metadata wiping. Herein, we present a method of random sanitization in DNA information storage using CRISPR-Cas12a (RSDISC) based on precise control of the thermodynamic energy of primer-template hybridization. We utilize the collateral cleavage (trans-activity) of single-stranded DNA (ssDNA) by CRISPR-Cas12a to achieve selective sanitization of files in metadata. This method enables ssDNA degradation with different GC content, lengths, and secondary structures to achieve a sanitization efficiency up to 99.9% for 28,258 oligonucleotides in DNA storage within one round. We demonstrate that the number of erasable files could reach 1011.7 based on a model of primer-template hybridization efficiency. Overall, RSDISC provides a random sanitization approach to set the foundation of information encryption, file classification, memory deallocation and accurate reading in DNA data storage.
Competing Interest Statement
There are patents pending on the DNA information storage (CA202310918094, CA202310366711, CA2024104301181)
Footnotes
↵* e-mail: songpingsjtu{at}sjtu.edu.cn
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