Tannic Acid Shaped Microbiome Composition in Midguts and Rearing Microcosms of Aedes triseriatus (Say)

Tannic Acid Shaped Microbiome Composition in Midguts and Rearing Microcosms of Aedes triseriatus (Say) | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Tannic Acid Shaped Microbiome Composition in Midguts and Rearing Microcosms of Aedes triseriatus (Say) Shicheng Chen, Liang Cui, Bin Zuo, Jiangchao Zhao, Edward D. Walker This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7706154/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Tannic acid (TA), a polyphenol derived from plants, often accumulates in water-holding containers where mosquitoes develop. Yet, its effects on mosquito gut microbiota remain poorly understood, representing an important knowledge gap. Because mosquito-associated microbiota are vital for host development, nutrition, and immunity, uncovering how TA shapes these microbial communities may yield new insights into mosquito biology and vector control strategies. In this study, we conducted a comparative analysis of bacterial communities in Aedes triseriatus midguts and rearing microcosms with or without TA supplement. Addition of TA at 0.35 mg/mL caused up to 50% larval Ae. triseriatus mortality, whereas combined supplementation with TA and kanamycin (100 µg/mL) increased mortality to 75% relative to controls. TA treatment significantly reduced microbial Chao 1 richness and Shannon diversity in larval and adult mosquito guts, while water and leaf samples were not affected. Distinct microbial community structures were observed between TA-treated and control groups across larvae, adults, water, and leaf surfaces. Pseudomonadota and Bacteroidota dominated all samples, with TA increasing the relative abundance of Pseudomonadota while decreasing Bacteroidota. Notably, Pseudomonas was enriched in TA-treated water, leaf surfaces, and larval midguts, suggesting a role in tannic acid detoxification. PICRUSt functional predictions indicated enrichment of carbohydrate and amino acid metabolism and membrane transport pathways under TA exposure, reflecting adaptive microbial responses to TA stress. Our findings highlighted how TA shapes mosquito microbiota and habitat quality, offering potential avenues to manipulate microbial communities as a biocontrol strategy for mosquito larvae. Biological sciences/Ecology Earth and environmental sciences/Ecology Biological sciences/Microbiology Biological sciences/Zoology Aedes triseriatus comparative microbiome analysis tannic acid diversity and function prediction Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7706154","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":532312585,"identity":"0eb29e79-d9a9-437c-9a1a-8f66318718e6","order_by":0,"name":"Shicheng Chen","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAq0lEQVRIiWNgGAWjYFACHiA2sGFgg7GJ1FKQRrKWD4cRbIJAvv3swccFBufz+CQSGB+8bSNCi8GZvGTjGQa3i9kkEpgN5xKlRYLHTJrH4HZim0QCmzQvMVrkZ/CY/+YxOAfSwv6bKC0MN3jMmHkMDoBtYSZKi8GZHGOgw5IT23geNkvOOUeMw9rPGH7m+WOXOL89+eCHN2XEOAwBGBtIUz8KRsEoGAWjADcAAJd6LmLmpHCWAAAAAElFTkSuQmCC","orcid":"","institution":"Northern Illinois University","correspondingAuthor":true,"prefix":"","firstName":"Shicheng","middleName":"","lastName":"Chen","suffix":""},{"id":532312593,"identity":"22bdcb43-9463-4086-9915-5f137de2bb6a","order_by":1,"name":"Liang Cui","email":"","orcid":"","institution":"Third Institute of Oceanography, MNR","correspondingAuthor":false,"prefix":"","firstName":"Liang","middleName":"","lastName":"Cui","suffix":""},{"id":532312595,"identity":"b365e6d9-8982-4be0-9c2a-d7bd80ea24c5","order_by":2,"name":"Bin Zuo","email":"","orcid":"","institution":"South China Agricultural University","correspondingAuthor":false,"prefix":"","firstName":"Bin","middleName":"","lastName":"Zuo","suffix":""},{"id":532312597,"identity":"182c8421-b205-42e6-9ffd-9b5d0c3e1752","order_by":3,"name":"Jiangchao Zhao","email":"","orcid":"","institution":"South China Agricultural University","correspondingAuthor":false,"prefix":"","firstName":"Jiangchao","middleName":"","lastName":"Zhao","suffix":""},{"id":532312599,"identity":"232c4850-51ba-4832-961f-3ee2e54834b5","order_by":4,"name":"Edward D. 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(Say)","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Aedes triseriatus, comparative microbiome analysis, tannic acid, diversity and function prediction","lastPublishedDoi":"10.21203/rs.3.rs-7706154/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7706154/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Tannic acid (TA), a polyphenol derived from plants, often accumulates in water-holding containers where mosquitoes develop. Yet, its effects on mosquito gut microbiota remain poorly understood, representing an important knowledge gap. Because mosquito-associated microbiota are vital for host development, nutrition, and immunity, uncovering how TA shapes these microbial communities may yield new insights into mosquito biology and vector control strategies. In this study, we conducted a comparative analysis of bacterial communities in Aedes triseriatus midguts and rearing microcosms with or without TA supplement. Addition of TA at 0.35 mg/mL caused up to 50% larval Ae. triseriatus mortality, whereas combined supplementation with TA and kanamycin (100 µg/mL) increased mortality to 75% relative to controls. TA treatment significantly reduced microbial Chao 1 richness and Shannon diversity in larval and adult mosquito guts, while water and leaf samples were not affected. Distinct microbial community structures were observed between TA-treated and control groups across larvae, adults, water, and leaf surfaces. Pseudomonadota and Bacteroidota dominated all samples, with TA increasing the relative abundance of Pseudomonadota while decreasing Bacteroidota. Notably, Pseudomonas was enriched in TA-treated water, leaf surfaces, and larval midguts, suggesting a role in tannic acid detoxification. PICRUSt functional predictions indicated enrichment of carbohydrate and amino acid metabolism and membrane transport pathways under TA exposure, reflecting adaptive microbial responses to TA stress. Our findings highlighted how TA shapes mosquito microbiota and habitat quality, offering potential avenues to manipulate microbial communities as a biocontrol strategy for mosquito larvae.","manuscriptTitle":"Tannic Acid Shaped Microbiome Composition in Midguts and Rearing Microcosms of Aedes triseriatus (Say)","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-10-27 11:33:32","doi":"10.21203/rs.3.rs-7706154/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"b1ea484a-69a6-4da6-961b-4fe120d36bd7","owner":[],"postedDate":"October 27th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":56582168,"name":"Biological sciences/Ecology"},{"id":56582169,"name":"Earth and environmental sciences/Ecology"},{"id":56582170,"name":"Biological sciences/Microbiology"},{"id":56582171,"name":"Biological sciences/Zoology"}],"tags":[],"updatedAt":"2026-01-07T11:40:19+00:00","versionOfRecord":[],"versionCreatedAt":"2025-10-27 11:33:32","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7706154","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7706154","identity":"rs-7706154","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}