Collinsella provencensis sp. nov., a new species identified from healthy human gut microbiota.

A Gram-positive and anaerobic bacterium was isolated from the stool sample of a healthy French volunteer. A taxonogenomics approach was adopted to characterize it. Cells are rod shaped non-spore-forming and non-motile. Growth of strain Marseille-P3740 occurs at 37°C in anaerobic atmosphere at pH 7. The 16S ribosomal RNA gene sequence analysis of the novel strain Marseille-P3740 displayed 96.31% similarity in nucleotide sequence with Collinsella intestinalis strain JCM 10643, the phylogenetically closest related species with standing in nomenclature. C 16:0 , C 18:1n9 and C 18:2n6 are the major cellular fatty acid components. The genomic of strain Marseille-P3740 T is 1.74 Mbp long with 59.1 mol% of G + C content. Based on the taxonogenomic description and the phenotypic and biochemical properties of this bacterium, we propose the strain Marseille-P3740T (= CSUR P3740 = CCUG 70947) as a new species, Collinsella provencensis sp. nov.