Study of Some Parameters of Recovered Sperm from Vitamin Ctreated Endometriotic Rats Model

The present study was designed to reveal the biochemical and parameters changes of the retrieval sperms uteri of surgically-induced endometriotic rats post 30 min and 1h of coupling. Seventy-five adult female rats were divided into 3 groups as follows: Group 1: 25 shame-operated female rats were orally administrated with 0.1 ml of d.w for 35 days. Group 2: 25 endometriotic female rats were orally administrated with 0.1 ml of d.w for 35 days. Groups 3: 25 endometriotic female rats were orally administrated with 200 mg/Kg of vitamin C for 35 days. At the end of administration, 10 female rats of each groups were mated with fertile males to study sperm parameters. Whereas, others 15 endometriotic female rats were left without mating to use their uteri for leukocytes counts, oxidative markers and PGF2α assay. The retrieval sperms from uteri of endometriotic rats (G2) were showed a significant (p≤0.05) lower in their functions parameters "concentration, viability, progressive motility, and the activity of LDH-C4 enzyme depending on the remaining them in the uteri compared to those values of retrieval sperms from control (G1) and unmated endometriotic rats that treated with vitamin C (G3). Also, the uterine flushing fluids of endometriotic rats (G2) exhibited that had a significant (p≤0.01) increase in the levels of leukocyte counts compared to those values of control (G1) and unmated endometriotic rats with vitamin C (G3). Whereas, there was no significant (P>0.05) difference in the levels of PGF2α in the uterine tissue of all unmated endometriotic groups (G1, G2 and G3). Surgically-induced endometriotic passive influence affected uterine milieu via an appearance of oxidative stress that increased significantly (p≤0.01) as a result of leukocytes count elevation. Ditto, there passive variables in the uterine microenvironment was reflected on the sperm functions parameters leading to infertility. While, the role of vitamin C in ameliorate of all above passive variants was evident.