Multiple stressor effects on organic carbon degradation and microbial community composition in urban river sediments in a mesocosm experiment

Multiple stressor effects on organic carbon degradation and microbial community composition in urban river sediments in a mesocosm experiment | bioRxiv /* */ /* */ <!-- <!-- /*! * yepnope1.5.4 * (c) WTFPL, GPLv2 */ (function(a,b,c){function d(a){return"[object Function]"==o.call(a)}function e(a){return"string"==typeof a}function f(){}function g(a){return!a||"loaded"==a||"complete"==a||"uninitialized"==a}function h(){var a=p.shift();q=1,a?a.t?m(function(){("c"==a.t?B.injectCss:B.injectJs)(a.s,0,a.a,a.x,a.e,1)},0):(a(),h()):q=0}function i(a,c,d,e,f,i,j){function k(b){if(!o&&g(l.readyState)&&(u.r=o=1,!q&&h(),l.onload=l.onreadystatechange=null,b)){"img"!=a&&m(function(){t.removeChild(l)},50);for(var d in y[c])y[c].hasOwnProperty(d)&&y[c][d].onload()}}var j=j||B.errorTimeout,l=b.createElement(a),o=0,r=0,u={t:d,s:c,e:f,a:i,x:j};1===y[c]&&(r=1,y[c]=[]),"object"==a?l.data=c:(l.src=c,l.type=a),l.width=l.height="0",l.onerror=l.onload=l.onreadystatechange=function(){k.call(this,r)},p.splice(e,0,u),"img"!=a&&(r||2===y[c]?(t.insertBefore(l,s?null:n),m(k,j)):y[c].push(l))}function j(a,b,c,d,f){return q=0,b=b||"j",e(a)?i("c"==b?v:u,a,b,this.i++,c,d,f):(p.splice(this.i++,0,a),1==p.length&&h()),this}function k(){var a=B;return a.loader={load:j,i:0},a}var l=b.documentElement,m=a.setTimeout,n=b.getElementsByTagName("script")[0],o={}.toString,p=[],q=0,r="MozAppearance"in l.style,s=r&&!!b.createRange().compareNode,t=s?l:n.parentNode,l=a.opera&&"[object Opera]"==o.call(a.opera),l=!!b.attachEvent&&!l,u=r?"object":l?"script":"img",v=l?"script":u,w=Array.isArray||function(a){return"[object Array]"==o.call(a)},x=[],y={},z={timeout:function(a,b){return b.length&&(a.timeout=b[0]),a}},A,B;B=function(a){function b(a){var a=a.split("!"),b=x.length,c=a.pop(),d=a.length,c={url:c,origUrl:c,prefixes:a},e,f,g;for(f=0;f<d;f++)g=a[f].split("="),(e=z[g.shift()])&&(c=e(c,g));for(f=0;f<b;f++)c=x[f](c);return c}function g(a,e,f,g,h){var i=b(a),j=i.autoCallback;i.url.split(".").pop().split("?").shift(),i.bypass||(e&&(e=d(e)?e:e[a]||e[g]||e[a.split("/").pop().split("?")[0]]),i.instead?i.instead(a,e,f,g,h):(y[i.url]?i.noexec=!0:y[i.url]=1,f.load(i.url,i.forceCSS||!i.forceJS&&"css"==i.url.split(".").pop().split("?").shift()?"c":c,i.noexec,i.attrs,i.timeout),(d(e)||d(j))&&f.load(function(){k(),e&&e(i.origUrl,h,g),j&&j(i.origUrl,h,g),y[i.url]=2})))}function h(a,b){function c(a,c){if(a){if(e(a))c||(j=function(){var a=[].slice.call(arguments);k.apply(this,a),l()}),g(a,j,b,0,h);else if(Object(a)===a)for(n in m=function(){var b=0,c;for(c in a)a.hasOwnProperty(c)&&b++;return b}(),a)a.hasOwnProperty(n)&&(!c&&!--m&&(d(j)?j=function(){var a=[].slice.call(arguments);k.apply(this,a),l()}:j[n]=function(a){return function(){var b=[].slice.call(arguments);a&&a.apply(this,b),l()}}(k[n])),g(a[n],j,b,n,h))}else!c&&l()}var h=!!a.test,i=a.load||a.both,j=a.callback||f,k=j,l=a.complete||f,m,n;c(h?a.yep:a.nope,!!i),i&&c(i)}var i,j,l=this.yepnope.loader;if(e(a))g(a,0,l,0);else if(w(a))for(i=0;i (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0];var j=d.createElement(s);var dl=l!='dataLayer'?'&l='+l:'';j.src='//www.googletagmanager.com/gtm.js?id='+i+dl;j.type='text/javascript';j.async=true;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-M677548'); Skip to main content Home About Submit ALERTS / RSS Search for this keyword Advanced Search New Results Multiple stressor effects on organic carbon degradation and microbial community composition in urban river sediments in a mesocosm experiment View ORCID Profile Daria Baikova , View ORCID Profile Una Hadziomerovic , View ORCID Profile Iris Madge Pimentel , View ORCID Profile Dominik Buchner , View ORCID Profile Anna-Maria Vermiert , View ORCID Profile Philipp M. Rehsen , View ORCID Profile Verena Brauer , View ORCID Profile Rainer U Meckenstock doi: https://doi.org/10.1101/2024.07.05.602289 Daria Baikova 1 University of Duisburg-Essen; Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Daria Baikova For correspondence: daria.baikova{at}uni-due.de Una Hadziomerovic 1 University of Duisburg-Essen; Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Una Hadziomerovic Iris Madge Pimentel 2 University Duisburg-Essen; Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Iris Madge Pimentel Dominik Buchner 1 University of Duisburg-Essen; Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Dominik Buchner Anna-Maria Vermiert 3 Ruhr University Bochum Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Anna-Maria Vermiert Philipp M. Rehsen 1 University of Duisburg-Essen; Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Philipp M. Rehsen Verena Brauer 1 University of Duisburg-Essen; Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Verena Brauer Rainer U Meckenstock 1 University of Duisburg-Essen; Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Rainer U Meckenstock Abstract Info/History Metrics Supplementary material Preview PDF Abstract Microorganisms in river sediments are the primarily responsible organisms for the turnover of dissolved organic carbon (DOC) in these systems and therefore are key players for river ecosystem functioning. Rivers are increasingly threatened by multiple stressors such as salinization and temperature rise, but little is known about how microbial DOC-degradation responds to these stressors and whether this function recovers after stressor release. Here, we investigated the direct and indirect effects of salinity and temperature increase and decrease on microbial communities and their ability to degrade DOC in river sediments using the outdoor experimental mesocosm system ExStream. Composition of sediment microbial communities was determined at the end of acclimatization, stressor, and recovery phase using 16S rRNA gene sequencing. At the same time points, DOC degradation rates were quantified in additional microcosm incubations based on isotopic changes of CO2 with the help of reverse stable isotope labelling. Our results showed that raising the salinity by 154.1 mg Cl- L-1 and temperature by 3.5 degrees Celsius did not affect DOC degradation during the stressor phase but significantly increased DOC degradation in the recovery phase after stressors were released. Likewise, microbial community composition stayed constant during acclimation and stressor phase, but became more diverse in the recovery phase. The results indicate that microbial community composition and functioning were resistant towards both stressors, but responded to stressor release due to indirect effects of stressor increase and release on the riverine food web. Competing Interest Statement The authors have declared no competing interest. Copyright The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license . View the discussion thread. Back to top Previous Next Posted July 07, 2024. Download PDF Supplementary Material Email Thank you for your interest in spreading the word about bioRxiv. NOTE: Your email address is requested solely to identify you as the sender of this article. Your Email * Your Name * Send To * Enter multiple addresses on separate lines or separate them with commas. You are going to email the following Multiple stressor effects on organic carbon degradation and microbial community composition in urban river sediments in a mesocosm experiment Message Subject (Your Name) has forwarded a page to you from bioRxiv Message Body (Your Name) thought you would like to see this page from the bioRxiv website. Your Personal Message CAPTCHA This question is for testing whether or not you are a human visitor and to prevent automated spam submissions. Share Multiple stressor effects on organic carbon degradation and microbial community composition in urban river sediments in a mesocosm experiment Daria Baikova , Una Hadziomerovic , Iris Madge Pimentel , Dominik Buchner , Anna-Maria Vermiert , Philipp M. Rehsen , Verena Brauer , Rainer U Meckenstock bioRxiv 2024.07.05.602289; doi: https://doi.org/10.1101/2024.07.05.602289 Share This Article: Copy Citation Tools Multiple stressor effects on organic carbon degradation and microbial community composition in urban river sediments in a mesocosm experiment Daria Baikova , Una Hadziomerovic , Iris Madge Pimentel , Dominik Buchner , Anna-Maria Vermiert , Philipp M. Rehsen , Verena Brauer , Rainer U Meckenstock bioRxiv 2024.07.05.602289; doi: https://doi.org/10.1101/2024.07.05.602289 Citation Manager Formats BibTeX Bookends EasyBib EndNote (tagged) EndNote 8 (xml) Medlars Mendeley Papers RefWorks Tagged Ref Manager RIS Zotero Tweet Widget Facebook Like Google Plus One Subject Area Microbiology Subject Areas All Articles Animal Behavior and Cognition (7643) Biochemistry (17717) Bioengineering (13910) Bioinformatics (42018) Biophysics (21480) Cancer Biology (18629) Cell Biology (25537) Clinical Trials (138) Developmental Biology (13392) Ecology (19935) Epidemiology (2067) Evolutionary Biology (24356) Genetics (15617) Genomics (22531) Immunology (17755) Microbiology (40438) Molecular Biology (17200) Neuroscience (88706) Paleontology (667) Pathology (2840) Pharmacology and Toxicology (4832) Physiology (7657) Plant Biology (15171) Scientific Communication and Education (2046) Synthetic Biology (4304) Systems Biology (9828) Zoology (2272)