Nude Mouse Models for Green fluorescence Human Endometriosis

【Objective】 To establish a novel noninvasive fluorescent animal model for endometriosis in vitro and in vivo. 【Methods】 Adenovirus encoding enhancing green fluorescent protein (Ad-eGFP) was used to transfect endometrial glandular cells and stromal cells (cells transfection and injection, Method No.1), and fragments (tissues transfection and injection, Method No. 2). Transfection efficiencies were compared between the two methods in vitro. Then GFP transfected glandular cells and stromal cells suspension were injected into nude mice subcutaneously (Method No.1), taking Method No.2 as a comparison. In vivo observation last for 25 days, and positive rates and duration times of fluorescent lesions were calculated. Histological examination was used to confirmed lesion formation. 【Results】 On the fifth day after injection, lesion positive rate of Method No.1 was 88.9%, which was statistically significantly higher than that of Method No.2 (22.2%), P = 0.015 0.05. The fluorescent positive duration of Method No.1 and No.2 were 12 ± 8 days and 7 ± 4 days. The structures of lesions were all identified as human original endometrium by histological examination, including HE staining and immunofluorescency. 【Conclusion】 Noninvasive animal model of endometriosis can be built up by subcutaneously injection of Ad-EGFP transfected endometrial glandular cells and stromal cells suspension with higher positive rate and longer observation time