Green Synthesis and Assessment of Antibacterial and Antiobiofilm Activities of Silver Nanoparticles Using Carthumas tinctorius Extract

Green Synthesis and Assessment of Antibacterial and Antiobiofilm Activities of Silver Nanoparticles Using Carthumas tinctorius Extract | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Green Synthesis and Assessment of Antibacterial and Antiobiofilm Activities of Silver Nanoparticles Using Carthumas tinctorius Extract Marwa T. Hamid, Nehia N. Hussein This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5691556/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 19 You are reading this latest preprint version Abstract Silver nanoparticles (AgNPs) synthesized using Carthamus tinctorius flower extract exhibit significant biomedical potential, particularly for their antibacterial and antibiofilm properties. Various characterization techniques, including UV-visible spectroscopy, SEM, TEM, zeta potential, FTIR, and XRD, confirmed the successful biosynthesis of AgNPs with spherical shapes, sizes ranging from 20 to 60 nm. The UV-visible spectrum displayed a peak at approximately 417 nm, indicating AgNP formation.Invitro antimicrobial activity of biosynthesized AgNPs was demonstrated against Staphylococcus haemolyticus, Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii across different concentrations (100 to 6.25 µg/mL). Results showed concentration-dependent bacterial inhibition, with the highest efficacy observed at 100 µg/mL, especially against E. coli and A.baumannii (inhibition zones of 16.66 mm and 16.33 mm, respectively). The study highlights the potential application of AgNPs as bioactive agents in biotechnology and pharmacology. Silver nanoparticles C.tinctorius Green synthesis Antibacterial activity Bio-nanotechnology. Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Revision requested 30 Jan, 2025 Reviews received at journal 24 Jan, 2025 Reviews received at journal 22 Jan, 2025 Reviews received at journal 22 Jan, 2025 Reviewers agreed at journal 22 Jan, 2025 Reviewers agreed at journal 21 Jan, 2025 Reviews received at journal 21 Jan, 2025 Reviewers agreed at journal 19 Jan, 2025 Reviewers agreed at journal 19 Jan, 2025 Reviewers agreed at journal 19 Jan, 2025 Reviewers agreed at journal 17 Jan, 2025 Reviewers agreed at journal 17 Jan, 2025 Reviewers agreed at journal 17 Jan, 2025 Reviewers agreed at journal 17 Jan, 2025 Reviewers agreed at journal 17 Jan, 2025 Reviewers invited by journal 17 Jan, 2025 Editor assigned by journal 08 Jan, 2025 Submission checks completed at journal 07 Jan, 2025 First submitted to journal 21 Dec, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5691556","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":399347608,"identity":"83cac2b9-84d2-4ec1-83a6-3cde56e0e546","order_by":0,"name":"Marwa T. 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