Oxidized ATM Protein Kinase Promotes the Proliferation of Breast CAF Through Upregulating the SCD1 Gene Expression by Activating Core Proliferation-Related Pathways

Abstract PurposeStearoyl-CoA desaturase 1 (SCD1) is a key rate-limiting enzyme involved in the biosynthesis of monounsaturated fatty acids, which mediates lipid metabolism and participates in the proliferation of cancer cells. As a redox sensor, ATM protein kinase is also involved in DNA repair and cell proliferation regulation. Previous studies have shown that oxidized ATM protein kinase can facilitates the proliferation of breast CAF via by regulating the cell glucose and protein metabolism. However, lipid metabolism is essential for cell proliferation. Whether the oxidized ATM protein kinase can regulate the expression of the SCD gene to promote the lipid metabolism is unknown.MethodsWe use immunohistochemical techniques to observe the level of SCD1 and oxidized ATM protein kinase, MTT Assay and flow cytometric analysis observes the proliferation of breast CAFs, and Western Blot techniques analyze the expression of SCD gene expression.ResultsThe expression levels of SCD1 protein expression in breast cancer were closely related to oxidized ATM expression (P < 0.05). And SCD promotes the proliferation of breast CAF cells. The oxidized ATM protein kinase can promote the proliferation of breast CAFs by upregulation of SCD protein expression levels.ConclusionOur results indicate that oxidized ATM protein kinase may play a central role in promoting the proliferation of breast CAF by coordinating glucose, protein and lipid metabolism. oxidized ATM can facilitate the breast CAFs Lipid metabolism by upregulating the SCD1 gene expression.