A wide range of chromosome numbers result from unreduced gamete production in Brassica juncea × B. napus (AABC) interspecific hybrids

A wide range of chromosome numbers result from unreduced gamete production in Brassica juncea × B. napus (AABC) interspecific hybrids | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Help Center Sign In Submit a Preprint Cite Share Download PDF Article A wide range of chromosome numbers result from unreduced gamete production in Brassica juncea × B. napus (AABC) interspecific hybrids Annaliese Mason, Charles Addo Nyarko, Elvis Katche, Mariana Baez, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4978520/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 30 Nov, 2024 Read the published version in Heredity → Version 1 posted 8 You are reading this latest preprint version Abstract The establishment of successful interspecies hybrids requires restoration of a stable “2n” chromosome complement which can produce viable “n” gametes. This may occur (rarely) via recombination between non-homologous chromosomes, or more commonly is associated with a doubling of parental chromosome number to produce new homologous pairing partners in the hybrid. The production of unreduced “2n” gametes (gametes with the somatic chromosome number) may therefore be evolutionarily useful by serving as a key pathway for the formation of new polyploid hybrids, as might specific mechanisms permitting recombination between non-homologous chromosomes. Here, we investigated chromosome complements and fertility in third generation interspecific hybrids (AABC) resulting from a cross between allopolyploids Brassica juncea (AABB) × B. napus (AACC) followed by self-pollination for two generations. Chromosome numbers ranged from 2n = 48 – 74 in the experimental population (35 plants), with 9 - 16 B genome chromosomes and up to 4 copies of A genome chromosomes. Unreduced gamete production leading to a putative genome structure of approximately AAAABBCC was hence predicted to explain the high chromosome numbers observed. Additionally, the estimation of nuclei number in post-meiotic sporads revealed a higher frequency of unreduced gametes (0.04 - 5.21%) in the third generation AABC interspecific hybrids compared to the parental Brassica juncea (0.07%) and B. napus (0.13%). Our results suggest that unreduced gamete production in the subsequent generations following interspecific hybridization events may play a critical role in restoration of more stable, fertile chromosome complements. Biological sciences/Genetics/Plant genetics/Polyploidy in plants Biological sciences/Genetics/Polyploidy/Polyploidy in plants Biological sciences/Evolution/Experimental evolution Biological sciences/Genetics/Plant genetics/Plant hybridization Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Full Text Additional Declarations There is no duality of interest Supplementary Files 20240824SupplementaryinformationAddoNyarkoetalUnreducedgametesAABC.pdf Supplemental Information Supplementary Figure 1: Mitotic metaphase chromosome spreads from the S1 parental hybrid resulting from the cross Brassica juncea × B. napus followed by one generation of self-pollination. Predicted chromosome number for a first-generation interspecific hybrid is 2n = 37 (AABC) chromosomes. Bar = 10 µm. Supplementary Figure 2: Identification of the number of copies of chromosomes A01 and C1 in the interspecific hybrids resulting from the cross Brassica juncea × B. napus followed by two generations of self-pollination. A) Parental Brassica napus with 2 A01 chromosomes (red) and 2 C1 chromosomes (Turquoise). B) Parental Brassica juncea with 2 A01 chromosomes. Chromosomes are counterstained with DAPI (blue). Bars = 10µm. Supplementary Figure 3: Identification of the A and C-genome chromosomes (green) with Brassica centromere probes CentBr1 and CentBr2 in the parental Brassica juncea (A) and B. napus (B). Chromosomes are counterstained with DAPI (blue). Bars = 10µm. Supplementary Figure 4: Distribution of the number of B and AC-genome chromosomes in the interspecific hybrids resulting from the cross Brassica juncea × B. napus followed by two generations of self-pollination. Cite Share Download PDF Status: Published Journal Publication published 30 Nov, 2024 Read the published version in Heredity → Version 1 posted Editorial decision: revise 07 Oct, 2024 Review # 2 received at journal 05 Oct, 2024 Review # 1 received at journal 26 Sep, 2024 Reviewer # 2 agreed at journal 19 Sep, 2024 Reviewer # 1 agreed at journal 11 Sep, 2024 Reviewers invited by journal 03 Sep, 2024 Editor assigned by journal 26 Aug, 2024 First submitted to journal 26 Aug, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4978520","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":345264326,"identity":"f4a6f8e9-1a1c-402c-955c-2364922d2118","order_by":0,"name":"Annaliese Mason","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABFUlEQVRIie2QsWrDMBRFZQTJUCteDYHkFxQCJdBS/4qEoV6UNtDFo6DgTKGrQ39CYMjsoNV0VqkHT57dzaGFViXQmqKajhl0BnER7/AuDwCL5VSp8E+eAOBweMzkb4V0lPk/lU6mXD+9ive42TdkdRl44Iy+tnEZiZd7DldxeQOGYW5S/PIp9Am+pluOsrFb1EtR7jlMi/oOuLV5jWJYK5LgHImxk8ilUHT9hhJJuc+wyZgqNm8J/giCHGWHQyIjrCiHR+W2MSlYsXO9JXcEQDtfT5KOwoy9ZlpZEBzSVKLdhVvI2fZLSQtJE7c2FpvoYqp5vwq89SZ7bmM5Hamo0heT9GEYVsY138DfH4P+eYvFYrH08An50Gdr8TkM0AAAAABJRU5ErkJggg==","orcid":"https://orcid.org/0000-0003-2701-7964","institution":"University of Bonn","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Annaliese","middleName":"","lastName":"Mason","suffix":""},{"id":345264327,"identity":"53c84005-d744-4fab-8a20-4e62aee243b2","order_by":1,"name":"Charles Addo Nyarko","email":"","orcid":"","institution":"University of Bonn","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Charles","middleName":"Addo","lastName":"Nyarko","suffix":""},{"id":345264328,"identity":"9a40634f-971b-4209-9289-de108e5abd12","order_by":2,"name":"Elvis Katche","email":"","orcid":"","institution":"Justus Liebig University Giessen","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Elvis","middleName":"","lastName":"Katche","suffix":""},{"id":345264329,"identity":"b12222d8-3690-4315-ba0e-1954e8b4cf80","order_by":3,"name":"Mariana Baez","email":"","orcid":"","institution":"University of Bonn","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Mariana","middleName":"","lastName":"Baez","suffix":""},{"id":345264330,"identity":"76f7c1c3-8424-43c1-b059-75b1dcc46acd","order_by":4,"name":"Zhenling Lv","email":"","orcid":"","institution":"University of Bonn","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Zhenling","middleName":"","lastName":"Lv","suffix":""}],"badges":[],"createdAt":"2024-08-26 14:02:06","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4978520/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4978520/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1038/s41437-024-00738-6","type":"published","date":"2024-11-30T05:00:00+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":65879552,"identity":"e397e0b8-3295-4f59-94d1-53b435f3947b","added_by":"auto","created_at":"2024-10-04 01:28:46","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":2591458,"visible":true,"origin":"","legend":"\u003cp\u003eI) Chromosome numbers (2n) in interspecific hybrids resulting from the \u0026nbsp;cross Brassica juncea × B. napus followed by two generations of self-pollination. Predicted chromosome number for a first-generation hybrid is 2n = 37 (AABC). \u0026nbsp;Estimated chromosome number for the second-generation (S1) parent was 2n = 44. \u0026nbsp;The most frequently observed chromosome number in the experimental \u0026nbsp;population was 2n = 71.\u003c/p\u003e\n\u003cp\u003eII) Representative mitotic chromosome spreads in interspecific hybrids resulting from the cross Brassica juncea × B. napus followed by two generations of self-pollination. Predicted chromosome number for a first-generation interspecific \u0026nbsp;hybrid is 2n = AABC = 37 chromosomes. A) Individual plant A-02-8 with 2n = 70 \u0026nbsp;chromosomes. B) Individual plant A-02-5 with 2n = 72 chromosomes. C) Individual plant A-02-27 with 2n = 50 chromosomes. D) Individual plant A-02-42 with 2n = 48 \u0026nbsp;chromosomes. E) Individual plant A-02-11 with 2n = 72 chromosomes. F) Individual plant A-02-25 with 2n = 69 chromosomes. Chromosomes were counterstained with \u0026nbsp;DAPI, (Gray). Bars = 10µm.\u003c/p\u003e","description":"","filename":"Figure1.tif.png","url":"https://assets-eu.researchsquare.com/files/rs-4978520/v1/eee1e179b40af3edf823bf36.png"},{"id":65879556,"identity":"73f5e3b0-e013-41a2-83de-48bb2d5f87cc","added_by":"auto","created_at":"2024-10-04 01:28:46","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":4874300,"visible":true,"origin":"","legend":"\u003cp\u003eIdentification of the number of copies of chromosomes A01 and C1 in \u0026nbsp;interspecific hybrids resulting from the cross Brassica juncea × B. napus followed \u0026nbsp;by two generations of self-pollination. A) Individual plant A-02-5 (2n =72) with \u0026nbsp;three A01 chromosomes (red) and three C1 chromosomes (green). B) Individual plant A-02-27 (2n = 50) with two A01 chromosomes and one C1 chromosome. C) \u0026nbsp;Individual plant A-02-18 (2n =73) with four A01 chromosomes and two C1 \u0026nbsp;35 chromosomes including a reciprocal translocation between chromosomes A01 and \u0026nbsp;C1 (inset with an increased size chromosome). D) Individual plant A-02-39 (2n =59) \u0026nbsp;with three A01 chromosomes and one C1 chromosome. Bars = 10µm.\u003c/p\u003e","description":"","filename":"Figure2.tif.png","url":"https://assets-eu.researchsquare.com/files/rs-4978520/v1/a7bce81f9918d1f801f227be.png"},{"id":65879557,"identity":"e5f7e7f7-2da2-46a2-9d03-b4170327c694","added_by":"auto","created_at":"2024-10-04 01:28:46","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":5461361,"visible":true,"origin":"","legend":"\u003cp\u003eIdentifying the B-genome (red), A and C-genome chromosomes (green) \u0026nbsp;in interspecific hybrids resulting from the cross Brassica juncea × B. napus \u0026nbsp;followed by two generations of self-pollination. A-C) Plant A-02-27 with ten B-genome chromosomes and 40 A/C genome chromosomes. D-F) Plant A-02-3 with \u0026nbsp;16 B-genome chromosomes and 58 A/C genome chromosomes. G-I) Plant A-02-05 \u0026nbsp;with 16 B-genome chromosomes and 56 A/C genome chromosomes. \u0026nbsp;Chromosomes were counterstained with DAPI (blue). Bars = 10µm.\u003c/p\u003e","description":"","filename":"Figure3.tif.png","url":"https://assets-eu.researchsquare.com/files/rs-4978520/v1/c6334d5088311e8770874437.png"},{"id":65879886,"identity":"7a74db8d-8b22-4daf-a103-7d0bcf5bf7a0","added_by":"auto","created_at":"2024-10-04 01:36:46","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":258359,"visible":true,"origin":"","legend":"\u003cp\u003eDistribution of the number of B and A/C-genome chromosomes in \u0026nbsp;interspecific hybrids resulting from the cross Brassica juncea × B. napus followed \u0026nbsp;by two generations of self-pollination. The modal B and A/C genome chromosome \u0026nbsp;numbers were 16 and 55 respectively.\u003c/p\u003e","description":"","filename":"Figure4.tif.png","url":"https://assets-eu.researchsquare.com/files/rs-4978520/v1/7fc0c2339f8fd5256f01c875.png"},{"id":65879888,"identity":"2f73ce81-facd-4cd4-ab4b-7799237c2669","added_by":"auto","created_at":"2024-10-04 01:36:46","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":6776901,"visible":true,"origin":"","legend":"\u003cp\u003eRepresentative sporad types observed in the experimental hybrids resulting from the cross Brassica juncea × B. napus followed by two generations \u0026nbsp;of self-pollination. A) Dyad, B) unbalanced tetrad with one large and three small \u0026nbsp;nuclei (yellow arrows), C) tetrad with an additional micronucleus, D) representative \u0026nbsp;meiotic behaviour at Anaphase II in the B. napus parental genotype “N5” showing \u0026nbsp;equal segregation of chromosomes, E-F) Anaphase II showing putative triads with \u0026nbsp;unequally segregated chromosomes. Bars = 10µm.\u003c/p\u003e","description":"","filename":"Figure5.tif.png","url":"https://assets-eu.researchsquare.com/files/rs-4978520/v1/f7a64ba150b5bb3d2f51103b.png"},{"id":65879887,"identity":"6a4e2c72-bb00-4b3f-9a70-145eb002703e","added_by":"auto","created_at":"2024-10-04 01:36:46","extension":"png","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":4436174,"visible":true,"origin":"","legend":"\u003cp\u003eKaryotype instability in the interspecific hybrids resulting from the cross \u0026nbsp;Brassica juncea × B. napus followed by two generations of self-pollination. Fluorescent in situ hybridisation (FISH) of mitotic and meiotic chromosome spreads \u0026nbsp;with B-genome genomic probe (purple) and A/C genome centromeric probe \u0026nbsp;(green). A-C) Mitotic chromosome showing chromosome number variation in the \u0026nbsp;experimental hybrids. A) Plant A-02-42 with nine B-genome chromosomes and 39 \u0026nbsp;A/C genome chromosomes. B) Plant A-02-44 with 16 B-genome chromosomes and \u0026nbsp;58 A/C genome chromosomes. C) Plant A-02-39 with 13 B-genome chromosomes \u0026nbsp;and 46 A/C genome chromosomes including a translocation involving \u0026nbsp;chromosomes from the B and A/C genomes (inset with size-inflated chromosomes, \u0026nbsp;yellow). D) Abnormal meiotic behaviour in the form of a non-homologous \u0026nbsp;chromosome association (yellow arrow, inset with an increased size chromosome \u0026nbsp;in yellow) at diakinesis. E) Lagging chromosomes (white arrows) at Anaphase II in \u0026nbsp;the S2 hybrids. F) Separation of sister chromatids instead of homologous \u0026nbsp;chromosomes at Anaphase I in an FDR-type 2n gamete formation. 16 B-genome \u0026nbsp;chromatids. Bar = 10 µm.\u003c/p\u003e","description":"","filename":"Figure6.tif.png","url":"https://assets-eu.researchsquare.com/files/rs-4978520/v1/6f1ad302c9829bcd685cacad.png"},{"id":65879553,"identity":"6bf888d8-3a29-42f7-b46b-bc3b5fd7126e","added_by":"auto","created_at":"2024-10-04 01:28:46","extension":"png","order_by":7,"title":"Figure 7","display":"","copyAsset":false,"role":"figure","size":677069,"visible":true,"origin":"","legend":"\u003cp\u003eA) Total number of self-pollinated seeds produced by interspecific \u0026nbsp;hybrids resulting from the cross B. napus × B. juncea followed by two generations \u0026nbsp;of self-pollination. Significant differences were observed between the \u0026nbsp;experimental hybrids and the two parental controls (Kruskal-Wallis test, p ˂ 0.001). B) Pollen viability of interspecific hybrids produced from the cross Brassica juncea \u0026nbsp;× Brassica napus followed by two generations of self-pollination compared to the \u0026nbsp;37 parental controls. There were no significant differences (p \u0026gt; 0.05) in pollen fertility \u0026nbsp;between the AABC hybrids and either the B. napus or B. juncea parental controls.\u003c/p\u003e","description":"","filename":"Figure7.tif.png","url":"https://assets-eu.researchsquare.com/files/rs-4978520/v1/f5a9cf6890f8b8e4e55d9ea7.png"},{"id":70269684,"identity":"6a909d29-2420-4316-a105-7667e1afaca5","added_by":"auto","created_at":"2024-12-01 08:07:05","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1950570,"visible":true,"origin":"","legend":"Article File","description":"","filename":"20240826UnreducedgametesfromBrassicajunceaXXB.napus.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4978520/v1_covered_20daf39a-971a-4cad-9713-10c7d5b4df48.pdf"},{"id":65879555,"identity":"d77b4410-736a-409f-a99b-558330142f3a","added_by":"auto","created_at":"2024-10-04 01:28:46","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":292129,"visible":true,"origin":"","legend":"\u003cp\u003eSupplemental Information Supplementary Figure 1: Mitotic metaphase chromosome spreads from the S1 parental hybrid resulting from the cross Brassica juncea × B. napus followed by \u0026nbsp;one generation of self-pollination. Predicted chromosome number for a first-generation interspecific hybrid is 2n = 37 (AABC) chromosomes. Bar = 10 µm. \u0026nbsp;Supplementary Figure 2: Identification of the number of copies of chromosomes \u0026nbsp;A01 and C1 in the interspecific hybrids resulting from the cross Brassica juncea × \u0026nbsp;B. napus followed by two generations of self-pollination. A) Parental Brassica \u0026nbsp;napus with 2 A01 chromosomes (red) and 2 C1 chromosomes (Turquoise). B) Parental Brassica juncea with 2 A01 chromosomes. Chromosomes are \u0026nbsp;counterstained with DAPI (blue). Bars = 10µm. Supplementary Figure 3: Identification of the A and C-genome chromosomes \u0026nbsp;(green) with Brassica centromere probes CentBr1 and CentBr2 in the parental \u0026nbsp;Brassica juncea (A) and B. napus (B). Chromosomes are counterstained with DAPI \u0026nbsp;(blue). Bars = 10µm. Supplementary Figure 4: Distribution of the number of B and AC-genome \u0026nbsp;chromosomes in the interspecific hybrids resulting from the cross Brassica juncea \u0026nbsp;× B. napus followed by two generations of self-pollination.\u003c/p\u003e","description":"","filename":"20240824SupplementaryinformationAddoNyarkoetalUnreducedgametesAABC.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4978520/v1/73de55a15ff980c2113aefe2.pdf"}],"financialInterests":"There is no duality of interest","formattedTitle":"A wide range of chromosome numbers result from unreduced gamete production in Brassica juncea × B. napus (AABC) interspecific hybrids","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"heredity","isNatureJournal":false,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"hdy","sideBox":"Learn more about [Heredity](http://www.nature.com/hdy/)","snPcode":"41437","submissionUrl":"https://mts-hdy.nature.com/cgi-bin/main.plex","title":"Heredity","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"ejp","reportingPortfolio":"Nature AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-4978520/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4978520/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"The establishment of successful interspecies hybrids requires restoration of a stable “2n” chromosome complement which can produce viable “n” gametes. This may occur (rarely) via recombination between non-homologous chromosomes, or more commonly is associated with a doubling of parental chromosome number to produce new homologous pairing partners in the hybrid. The production of unreduced “2n” gametes (gametes with the somatic chromosome number) may therefore be evolutionarily useful by serving as a key pathway for the formation of new polyploid hybrids, as might specific mechanisms permitting recombination between non-homologous chromosomes. Here, we investigated chromosome complements and fertility in third generation interspecific hybrids (AABC) resulting from a cross between allopolyploids Brassica juncea (AABB) × B. napus (AACC) followed by self-pollination for two generations. Chromosome numbers ranged from 2n = 48 – 74 in the experimental population (35 plants), with 9 - 16 B genome chromosomes and up to 4 copies of A genome chromosomes. Unreduced gamete production leading to a putative genome structure of approximately AAAABBCC was hence predicted to explain the high chromosome numbers observed. Additionally, the estimation of nuclei number in post-meiotic sporads revealed a higher frequency of unreduced gametes (0.04 - 5.21%) in the third generation AABC interspecific hybrids compared to the parental Brassica juncea (0.07%) and B. napus (0.13%). Our results suggest that unreduced gamete production in the subsequent generations following interspecific hybridization events may play a critical role in restoration of more stable, fertile chromosome complements.","manuscriptTitle":"A wide range of chromosome numbers result from unreduced gamete production in Brassica juncea × B. napus (AABC) interspecific hybrids","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-10-04 01:28:41","doi":"10.21203/rs.3.rs-4978520/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"revise","date":"2024-10-07T08:38:36+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"This content is not available.","date":"2024-10-05T11:42:10+00:00","index":2,"fulltext":"This content is not available."},{"type":"editorInvitedReview","content":"This content is not available.","date":"2024-09-26T17:56:46+00:00","index":1,"fulltext":"This content is not available."},{"type":"reviewerAgreed","content":"This content is not available.","date":"2024-09-19T18:41:13+00:00","index":2,"fulltext":"This content is not available."},{"type":"reviewerAgreed","content":"This content is not available.","date":"2024-09-11T11:49:45+00:00","index":1,"fulltext":"This content is not available."},{"type":"reviewersInvited","content":"","date":"2024-09-03T14:25:55+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-08-26T13:59:56+00:00","index":"","fulltext":""},{"type":"submitted","content":"Heredity","date":"2024-08-26T13:59:55+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"heredity","isNatureJournal":false,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"hdy","sideBox":"Learn more about [Heredity](http://www.nature.com/hdy/)","snPcode":"41437","submissionUrl":"https://mts-hdy.nature.com/cgi-bin/main.plex","title":"Heredity","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"ejp","reportingPortfolio":"Nature AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"4f2a090d-372c-4ef5-a3a2-c5cb6a0e4776","owner":[],"postedDate":"October 4th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":36594590,"name":"Biological sciences/Genetics/Plant genetics/Polyploidy in plants"},{"id":36594591,"name":"Biological sciences/Genetics/Polyploidy/Polyploidy in plants"},{"id":36594592,"name":"Biological sciences/Evolution/Experimental evolution"},{"id":36594593,"name":"Biological sciences/Genetics/Plant genetics/Plant hybridization"}],"tags":[],"updatedAt":"2024-12-01T08:06:40+00:00","versionOfRecord":{"articleIdentity":"rs-4978520","link":"https://doi.org/10.1038/s41437-024-00738-6","journal":{"identity":"heredity","isVorOnly":false,"title":"Heredity"},"publishedOn":"2024-11-30 05:00:00","publishedOnDateReadable":"November 30th, 2024"},"versionCreatedAt":"2024-10-04 01:28:41","video":"","vorDoi":"10.1038/s41437-024-00738-6","vorDoiUrl":"https://doi.org/10.1038/s41437-024-00738-6","workflowStages":[]},"version":"v1","identity":"rs-4978520","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4978520","identity":"rs-4978520","version":["v1"]},"buildId":"zQwnuV7TCBrMSSSToR1PI","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}