Growth characteristics of Myco-Composites Produced Using Locally Isolated Ganoderma Species and Lignocellulosic Waste

2025 · doi:10.1101/2025.11.24.690336
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The paper studied the growth characteristics and resulting properties of myco-composites made by culturing locally isolated Ganoderma species on different lignocellulosic waste substrates (paper, cardboard, sawdust, and wood shavings) with eight substrate formulations. Across formulations, fine fibrous substrates such as paper and cardboard produced the most uniform mycelial colonization and higher composite densities (0.45 and 0.41 g/cm3), whereas coarse or mixed substrates including wood shavings yielded lighter, less dense composites (about 0.27 g/cm3), with volumetric shrinkage varying from 7.1% to 26.7% depending on substrate type. FTIR spectroscopy confirmed fungal and lignocellulosic functional groups in the composites. The study limitation is that it focuses on material growth/structure metrics rather than biological mechanisms or health outcomes. The paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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Abstract

This study reports the development of myco-composites from locally isolated Ganoderma species and regional lignocellulosic wastes such as paper, cardboard, sawdust, and wood shavings. Eight substrate formulations were evaluated for fungal colonization and composite quality. Visual inspection revealed that fine, fibrous substrates like paper and cardboard achieved the most uniform mycelial colonization and highest densities (0.45 and 0.41 g/cm 3 , respectively), while coarse, porous substrates such as wood shavings and mixed substrates (M1 and M2) produced lighter, less dense composites (0.27 g/cm 3 ). Volumetric shrinkage ranged from 7.1% to 26.7%, with lower values in cardboard and higher in wood shavings. FTIR spectroscopy confirmed the presence of characteristic fungal and lignocellulosic functional groups in mixed composites. These findings demonstrate that composite density, dimensional stability, and chemical structure are strongly influenced by substrate type and proportion, highlighting the potential for tailored myco-composite materials using local waste resources. Graphical Abstract
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Abstract This study reports the development of myco-composites from locally isolated Ganoderma species and regional lignocellulosic wastes such as paper, cardboard, sawdust, and wood shavings. Eight substrate formulations were evaluated for fungal colonization and composite quality. Visual inspection revealed that fine, fibrous substrates like paper and cardboard achieved the most uniform mycelial colonization and highest densities (0.45 and 0.41 g/cm3, respectively), while coarse, porous substrates such as wood shavings and mixed substrates (M1 and M2) produced lighter, less dense composites (0.27 g/cm3). Volumetric shrinkage ranged from 7.1% to 26.7%, with lower values in cardboard and higher in wood shavings. FTIR spectroscopy confirmed the presence of characteristic fungal and lignocellulosic functional groups in mixed composites. These findings demonstrate that composite density, dimensional stability, and chemical structure are strongly influenced by substrate type and proportion, highlighting the potential for tailored myco-composite materials using local waste resources. Competing Interest Statement The authors have declared no competing interest.

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