Quantification of free sialic acids, Effect of EHF therapy in pulmonary edema: a possible alternative in the fight against COVID-19

The relevance of the problem of therapy and prevention of SARS-CoV-2 is associated with the development of pulmonary edema, the lack of rehabilitation methods and accurate diagnostic indicators of pulmonary edema. In this regard, the objective of this work was to evaluate the level of free sialic acids and the therapeutic effect of EHF therapy in rats with pulmonary edema. The work was carried out on 14 sexually mature female Wistar rats weighing 150-200g, divided into 3 groups. An EHF therapy biocorrector (CEM-TECH, 40–43 GHz, Russia) was used for 10 days for treatment. A standard reaction method with 2-thiobarbituric acid was used for the determination of free sialic acids. In the control group, the level of free sialic acids was increased by 2 times (0.420 ± 0.011 µmol / ml). In the experimental group, EHF therapy reduced the level of free sialic acids by 1.8 times (0.235 ± 0.043 µmol / ml). The level of free sialic acids increases with the development of pulmonary edema. This result may suggest the use of this indicator in addition to the known traditional methods of diagnosis and prognosis of pulmonary edema, as well as in pulmonary rehabilitation in patients infected with SARS-CoV-2.