Silicon nanocolumn-based disposable and flexible ultrasound patches

Silicon nanocolumn-based disposable and flexible ultrasound patches | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Silicon nanocolumn-based disposable and flexible ultrasound patches Byung Chul Lee, Dong-Hyun Kang, Seonghun Cho, Hae Youn Kim, Shinyong Shim, and 9 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5309768/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 18 Jul, 2025 Read the published version in Nature Communications → Version 1 posted You are reading this latest preprint version Abstract Traditional wearable ultrasound devices pose challenges concerning the rigidity and environmental impact of lead-based piezoelectric materials. This study proposes a novel silicon nanocolumn capacitive micromachined ultrasonic transducer (snCMUT) array for real-time wearable ultrasound imaging in disposable patches. Using a lead-free design, snCMUT incorporates silicon nanocolumns to address existing issues and achieves high transmission efficiency (220 kPa/V), flexibility, and low power consumption. The unique structure of snCMUT enhances displacement efficiency, enabling high-resolution imaging while maintaining a thin, flexible form factor (~ 900 µm). Phantom imaging demonstrates its superior performance, with high axial and lateral resolutions (0.52 and 0.55 mm) and depth penetration (~ 70 mm) at low voltage (8.9 V PP ). Upon successful application to monitor both sides of the human carotid arteries, snCMUT offers clear ultrasound images and continuous blood pressure waveform monitoring. This proposed innovation presents significant potential for continuous medical imaging and cardiovascular health assessment, addressing environmental concerns and reducing manufacturing costs (< $ 20). Physical sciences/Nanoscience and technology/Nanoscale devices/NEMS Health sciences/Health care/Medical imaging/Ultrasonography Physical sciences/Engineering/Electrical and electronic engineering Physical sciences/Engineering/Biomedical engineering wearable ultrasound imager disposable patch capacitive micromachined ultrasonic transducer silicon nanocolumns blood pressure monitoring Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Main Ultrasound is a widely used medical imaging modality, providing non-invasive and safe real-time imaging of deep-lying tissues, organs, and blood flow 1 – 3 . Compared to X-ray, computed tomography (CT), and magnetic resonance imaging (MRI), ultrasound machines are relatively compact and inexpensive 3 – 5 . Recent advancements in wearable devices and technology have led to the development of wearable ultrasonic transducer arrays 1 , 4 , 6 – 15 , which enable various applications, including monitoring blood flow in different body regions, imaging cardiovascular structures, and mapping tissue moduli. However, wearable ultrasound devices pose issues related to their inherent material limitations. First, as the primary component of wearable ultrasound devices, piezoelectric materials predominantly containing lead pose environmental issues and violate the Restriction of Hazardous Substances Directive (RoHS). Second, the inherent properties of piezoelectric materials necessitate thick backing layers to define ultrasound propagation direction and minimize pulse duration while quickly damping vibrations 14 , 16 . When backing layers are insufficient, both axial and lateral resolution worsen due to the ringing and reverberation of transducers. The thick layers make them unsuitable for wearable ultrasound technology, especially wearable patches for continuous monitoring, as they introduce discomfort when attached to the body. Third, assembling rigid piezoelectric materials with flexible electrodes to achieve conformal contact becomes increasingly difficult as array size and density increase due to fabrication based on blade dicing 4 , 17 . Thus, obstacles arise in medical imaging fields requiring narrow pitch, minimized kerf, and numerous elements to achieve high-resolution imaging attributable to the transducer beam width. Last, the high production cost of ultrasound transducer arrays typically necessitates the reuse of wearable patch-type ultrasound probes, driven by economic challenges 16 , 18 . While the reuse of ultrasound probes with disposable medical cover is standard in medical imaging, primarily due to the high production cost and environmental issues associated with lead-based piezoelectric materials, the concept of disposable ultrasound probes offers compelling advantages to wearable patches. Disposable ultrasound probes address hygiene, infection control, and cross-contamination issues in medical environments, thereby alleviating the burden of cleaning and sterilization between uses and offering convenience and cost-effectiveness to healthcare providers and patients. Particularly, disposable ultrasound probes enhance patient safety and eliminate the need for sterilization to streamline diagnostic procedures in environments following stringent hygiene protocols. Based on silicon wafers and semiconductor fabrication technology, capacitive micromachined ultrasonic transducers (CMUTs) offer a lead-free alternative to piezoelectric material-based ultrasound transducers with considerable potential for medical imaging, as they boast high element density and enable miniaturization advantages 2 , 3 , 19 . Free from lead, CMUTs circumvent environmental regulations, while batch production capabilities drastically reduce manufacturing costs even with high element density (under $ 12, Supplementary Table 1), facilitating the realization of disposable ultrasound probe patches. Moreover, CMUTs offer superior sensitivity, image resolution, and bandwidth compared to conventional piezoelectric material-based ultrasound probes 2 , 3 , 20 . Unlike piezoelectric transducers that experience degraded piezoelectric characteristics and signal-to-noise ratio (SNR) due to their rising temperature during long-term use, CMUTs avoid temperature increases even during extended use, making them more suitable for wearable patches intended for long-term monitoring 21 . However, despite the potential of CMUTs for high-resolution ultrasound imaging in wearable patches, their adoption faces challenges, including low output pressure and high bias voltage, which limit imaging depth and elevate driving voltage 22 , 23 . This study presents a flexible CMUT with exceptional pressure output, designed for real-time wearable ultrasound imaging in disposable patches (Fig. 1 a). Leveraging semiconductor and micromachining technologies, the designed lead-free ultrasound patches based on silicon nanocolumn capacitive micromachined ultrasonic transducer (snCMUT) array are disposable and environmentally friendly, with minimized fabrication costs of under $ 20 (Supplementary Table 1). The skin-conformal ultrasound patch comprises individual flexible CMUT elements separated by elastomer-filled trenches, mounted on an in-house designed flexible printed circuit board (FPCB), and encapsulated with polydimethylsiloxane (PDMS) (Fig. 1 b). To enhance transmission efficiency and reduce power consumption, silicon nanocolumns are utilized as springs beneath the moving plate of snCMUT, increasing the average displacement (Fig. 1 c). Fabricated using a rigid-island technique, the snCMUT array boasts high flexibility (minimum radius of curvature: <1 mm), stretchability (up to 140% without degradation), and minimized thickness (~ 900 µm fully packaged), making it ideal for wearable applications (Figs. 1 d and 1 e). To demonstrate the capabilities of disposable snCMUT patches, the human carotid artery is monitored on both sides of the neck in real-time. Owing to its elevated transmission efficiency, disposable snCMUT patches produce clear ultrasound images (B-mode for sonography and M-mode for blood pressure wave, Fig. 1 f) even at low driving voltages (8.9 V PP , Supplementary Tabel 2). The cost-effective disposable snCMUT patches enable multi-point monitoring of blood pressure waveforms, offering significant clinical advantages for cardiovascular health assessment and management. By monitoring both carotid arteries simultaneously, disposable snCMUT patches assist in identifying hypertensive patients missed with single-side measurements, detecting asymmetrical arterial stiffness, guiding precise treatment plans, enhancing patient safety, and supporting surgical planning and postoperative evaluation 24 , 25 . Fabrication of flexible snCMUT arrays The proposed snCMUT operates on a principle different from that of conventional CMUTs, which rely on the deformation of a thin plate 19 , 20 . In the design, snCMUTs use a thin plate with pistons driven by silicon nanocolumns as a spring 26 , 27 . The structure introduces a spring constant in the mass–spring–damper system, enhancing average displacement and packing density through parallel piston movement and large unit cells, respectively (Figs. 2 a and 2 b, left). Finite element analysis (FEA) simulations provide design parameters of the CMUT structure with silicon nanocolumns, aiming for a resonant frequency of 5 MHz for carotid artery imaging (Figs. 2 a and 2 b, right). Conventional CMUTs offer a pull-in voltage of 93 V, while snCMUT exhibits the pull-in voltage of 105 V. Below the pull-in voltage, snCMUT shows higher displacement and more efficient output pressure due to a larger unit cell area. The design includes 20 piston top plates forming a CMUT element, each affixed with nine 2 µm long silicon nanocolumns. To integrate vertically oriented silicon nanocolumns into CMUTs, precise fabrication with specific dimensions is necessary. Thus, compatibility with CMUT fabrication is crucial, favouring a top-down approach via silicon wafer etching over a bottom-up approach. Using deep reactive ion etching (DRIE) and reactive ion etching (RIE), silicon nanocolumns were fabricated with fixed central diameters of 1.5 µm and varying doughnut-shaped widths to control etch rates (Fig. 2 c). Silicon microcolumns of 1.45 µm diameter and scalloped sidewalls were fabricated using DRIE (Fig. 2 d, Supplementary Fig. 1a). While fabricating nanocolumns by reducing the silicon microcolumn size through RIE undercutting, precise control of the vertical and horizontal etch rate ( 984 and 758 Å/min, respectively, for this study) was crucial. The uniformity of etching anisotropy was confirmed even with increased etching time, and the undercut was controlled by varying the opening area (W) and etch time (Supplementary Fig. 2). Successful top-down fabrication of silicon nanocolumns (Fig. 2 e, Supplementary Fig. 1b-d) with diameters controlled by etch time was achieved (Fig. 2 f). The mechanical properties of the silicon nanocolumns, acting as springs, were evaluated using a pico-indenter. Smaller diameter nanocolumns contracted more, with 300 nm diameter nanocolumns changing length by 148 nm under 500 µN (Fig. 2 g). The uniaxial compressive strain recorded was 9.1%. Silicon nanocolumns with diameters below 100 nm were unstable, leading to the collapse of the top SiO 2 layer (Supplementary Fig. 3a). Assuming diameters of 500–700 nm to be optimal for stability and mechanical performance in snCMUT, fabrication was equally well-defined in silicon-on-insulator (SOI) wafers with high repeatability (Supplementary Fig. 3b). The snCMUT array was fabricated via wafer bonding of two SOI wafers using conventional microfabrication technologies (Fig. 2 h, Supplementary Fig. 4). For wearable applications, PDMS was filled into trenches between elements singulated on both sides during DRIE for different trench widths to maximize flexibility (Supplementary Fig. 5). The cross-sectional cutting plane images obtained via a focused ion beam (FIB) confirmed the well-bonded silicon nanocolumns (Figs. 2 i– 2 j) and formed vacuum gaps (Fig. 2 k). Transmission electron microscope (TEM) images of the wafer bonding interface (Fig. 2 l) and the interface between SiO 2 grown by thermal oxidation and Si (Fig. 2 m) confirmed the successful bonding of silicon nanocolumns and the Si plate of CMUT via wafer fusion bonding of two SOI wafers. The snCMUT array demonstrated impressive flexibility and stretchability, achieving bending with a radius of curvature less than 1 mm and elongation up to 140%, stretching from 40 mm to over 55 mm (Supplementary Fig. 6). The obtained high degree of stretchability prevented wearable snCMUT patch degradation, as the snCMUT array integrated into the FPCB avoided strain mismatch from tensile and compressive forces during bending. Characterization of snCMUT To evaluate the characteristics of the fabricated snCMUT array, a comprehensive assessment of amplitude and phase components of the electrical input impedance was performed using an impedance analyser. Despite slight changes in the phase and amplitude of the electrical input impedance, respectively, the fabricated snCMUT exhibited sharp changes in both phase and amplitude at the resonant frequency, as analysed using DC voltage increasing from 10 to 50 V (Figs. 3 a and 3 b). Since the silicon nanocolumns functioned as springs, the shift in impedance phase with increasing bias voltage in snCMUTs was minimized compared to conventional CMUTs, which are affected by static deflections 22 , 26 . An average resonant frequency of 6.7 MHz was observed in air at 50 V. The dynamic response of the snCMUT elements was evaluated using a laser Doppler vibrometer (LDV) integrated with a custom-built microscope. With a DC bias voltage of 50 V and a continuous wave pulse of 1 VPP at 4.7 MHz, LDV recorded the piston movement of the top plate along the z-axis (Fig. 3 c). Maximum displacements of 3.20 and − 3.64 nm were recorded in the positive and negative z-axis directions, respectively, indicating the balanced movement of the piston top plate. The balanced movement implied well-fabricated silicon nanocolumns and embedded snCMUTs. The peak-to-peak displacement of four-piston top plates is shown in Fig. 3 d. Variations in displacement were attributed to differences in the diameter of silicon nanocolumns fabricated via the top-down approach. The average volume displacement efficiency ( u avg ) was calculated as 22 $$\:{u}_{avg}=\frac{1}{{A}_{tot}}{\int\:}_{{A}_{tot}}^{\:}udA$$ 1 where u denotes the corresponding displacement, and A tot represents the total plate area. The average volume displacement efficiency for the four cells was 6.86, 6.84, 3.92, and 5.34 nm/V PP , with a total average of 5.74 nm/V under 50 V DC bias and 1 V PP AC wave, which was five times higher than those reported previously. To compare the displacement profiles of the top plate during movement in conventional CMUTs and snCMUTs, the cross-sectional profile of snCMUT was used (Fig. 3 e). After normalising the cell dimensions and maximum displacement, the areas with 70% maximum displacement comprised 31% conventional CMUTs and 63% snCMUTs. Thus, snCMUTs generated top-plate movement over a broader area, resulting in higher average displacement efficiency and consequent greater transmission efficiency. The maximum output pressure and transmission efficiency of snCMUT were evaluated using a hydrophone in corn oil. The hydrophone with a pre-amplifier and oscilloscope monitored the pressure field of 3.5 mm away from the snCMUT elements with a DC bias voltage of 50 V and a 5 V PP AC wave at 4.65 MHz. Transient pressure amplitudes of 16.7, 27.5, and 37.5 kPa for 1, 4, and 10 channels, respectively, are shown in Fig. 3 f. The calculated maximum transient pressure at the surface was 1.51 MPa peak-to-peak, achieved with 5-cycle pulses at 4.65 MHz and 5 V PP . Approximately five cycles were necessary to transition from the asymmetric motion of the piston top plate, caused by the varying spring constants of individual silicon nanocolumns due to fabrication-related variances, to a symmetric motion influenced by the weight of the piston top plate (Supplementary Fig. 7). Herein, snCMUT exhibited a − 3dB fractional bandwidth (FBW) of 31.61% at 4.65 MHz, which was lower than that of conventional CMUTs due to silicon nanocolumn fabrication variations and the weight of the piston top plate (Fig. 3 g). However, the − 6dB FBW of the frequency spectrum in pulse-echo response widened to 68.83% at 3.69 MHz due to damping by the lens after packaging snCMUT in a custom-made FPCB with a PDMS lens (Fig. 3 h). Therefore, variations in silicon nanocolumns and higher FBW could be achieved by using advanced lithography techniques. Comparative analyses of snCMUT with commercial lead zirconate titanate (PZT) probes, conventional CMUT, and enhanced CMUT (Supplementary Table 3) were conducted. Maximum pressure at the transducer surface was calculated to mitigate the impact of varying aperture sizes and focal lengths, accounting for acoustic attenuation and lens-induced gain. Commercial PZT transducers and conventional CMUT exhibited transmission efficiencies of 13.4 and 6.30 kPa/V, respectively 22 , 28 . Previous efforts improved CMUT transmission efficiency to 21.5 kPa/V using post-CMUTs 22 and 61.2 kPa/V using electrodepost CMUTs (EP-CMUT) 23 . Herein, snCMUT achieved a transmission efficiency of 220 kPa/V, a tenfold increase over post-CMUT and 3.6-fold over EP-CMUT. The parallel movement of the piston top-plate facilitated by silicon nanocolumns boosted the efficiency, enhancing volume displacement and transmission efficiencies with low voltage requirements. Thus, the proposed ultrasound transducers were proven to be highly suitable for wearable ultrasound technologies, offering high performance, low energy consumption, environmental adaptability, and disposability. Comparative study on the ultrasound imaging performance of snCMUT on phantom Conventional CMUTs directly connected to imaging systems generally suffer from degraded SNR due to large capacitances in flexible cables (hundreds of pF) 29 – 31 . To mitigate this issue, conventional CMUTs typically use application-specific integrated circuits (ASICs) placed close to the CMUT to improve SNR 29 – 31 . However, ASICs are expensive and present a significant challenge in developing wearable and disposable ultrasound patches. In contrast, snCMUT achieved sufficient SNR through its high transmission efficiency, eliminating the requirement for ASICs. Thus, system complexity and cost were reduced, along with lower power consumption, making snCMUT advantageous for use in wearable and disposable ultrasound patches. The fabricated disposable snCMUT patches included a custom-made FPCB directly connected to the Vantage 256 research ultrasound system, enabling simultaneous non-invasive monitoring of multiple body locations. The packaged patch was thin (933 µm, Supplementary Fig. 8) and suitable for attachment to the human body (Fig. 1 f). An in-house imaging code was developed to reconstruct the acquired images. To correct positional changes of each element in the wearable ultrasound patch in real-time, a technique from a previously published patent was used to detect relative positions by measuring capacitance changes between each singulated CMUT element (Supplementary Fig. 9). Image reconstruction was performed using a plane-wave compounding algorithm with 57 angles (Supplementary Fig. 10). To evaluate the wearable ultrasound imaging capabilities of the snCMUT patches, a multi-purpose ultrasound phantom (model ATS-539, CIRS) was used, combining monofilament line targets for distance measurements and tissue-mimicking target structures of varying sizes and contrasts (Supplementary Fig. 11a). For a comparative illustration of resolution between the disposable snCMUT patch and a conventional CMUT probe, images were captured in real-time up to a depth of 90 mm (Figs. 4 a and 4 b). The conventional CMUT fabricated via the wafer bonding method was optimized for imaging by adjusting the DC bias voltage and AC voltage frequencies, operating at 70 V and 30.6 V PP at 6.25 MHz, respectively. The ultrasound images from the conventional CMUT showed axial-lateral resolution and anechoic targets up to a depth of 40 and 20 mm, respectively (Fig. 4 c, Supplementary Fig. 11b). The disposable snCMUT patch operated at a lower voltage compared to the conventional CMUT (DC bias voltage of 40 V and AC voltage of 8.9 V PP at 4.25 MHz). After packaging, the resonant frequency shifted from 4.65 to 4.25 MHz due to the damping effect of the PDMS lens covering the piston top plate. Ultrasound images from the snCMUT patches demonstrated the ability to resolve axial-lateral resolution targets at depths of approximately 75 mm, even at low voltages (Figs. 4 d and 4 e, Supplementary Fig. 11c). The anechoic target structure was distinguishable up to a depth of 30 mm. While analyzing ultrasound image intensity in the axial direction, the conventional CMUT showed the lowest background noise of approximately 18 mm and failed to distinguish the wire target beyond 40 mm. Conversely, the disposable snCMUT patch exhibited the lowest background noise at approximately 40 mm and produced distinguishable images up to 70 mm (Fig. 4 f). The disposable snCMUT patch achieved outstanding axial and lateral resolutions of 0.52 and 0.55 mm, respectively, in a 100 µm nylon monofilament phantom. Both the conventional CMUT probe and the disposable snCMUT patch demonstrated excellent axial and lateral resolutions below 1 mm compared to the commercial PZT-based probe. However, due to lower transmission efficiency, the conventional CMUT experienced a blurred image beyond 40 mm, while the snCMUT patch maintained sub-1 mm resolutions up to 70 mm (Figs. 4 g and 4 h). Furthermore, the snCMUT patch displayed higher ultrasound image contrast than the conventional CMUT probe, attributed to its superior SNR resulting from higher transmission efficiency (Fig. 4 i). The superior performance of the disposable snCMUT patch was established by comparing it with previously reported wearable ultrasound probes (Supplementary Table 2). Recently published wearable ultrasound probes have achieved high frequencies and relatively high axial and lateral resolution in rigid forms, but their thickness exceeds 3 mm, limiting their applicability in wearable devices 14 . Stretchable ultrasound probes, while relatively thin at 1 mm, suffer from poor lateral resolution of 2 mm due to wide pitch and kerf 4 , 13 . Probes using PVDF are highly thin (0.1 mm) and flexible, but they require a high working voltage (95 VPP), trans-impedance amplifiers, and digitized amplification 6 . Contrarily, the disposable snCMUT patch is stretchable (excluding the FPCB) and fully packaged at less than 1 mm thickness. The disposable snCMUT patch achieved very high imaging quality, with axial and lateral resolutions of less than 0.55 mm, even at a very low working voltage of 8.9 V PP, owing to its high transmission efficiency and no requirements for ASICs to improve SNR. Real-time imaging and blood pressure waveform monitoring from carotid artery After evaluating and optimizing ultrasound images of the disposable snCMUT patches using a commercial multi-purpose phantom, the patches were applied to perform real-time monitoring of the carotid artery in the human neck (Fig. 5 a). Cardiovascular diseases, including heart conditions such as arrhythmia and vascular issues from cholesterol buildup, can disrupt blood flow in the carotid artery, leading to strokes 32 , 33 . Continuous monitoring of the artery is crucial for preventing such events. Wearable ultrasound patches offer continuous, non-invasive monitoring of arterial health, providing critical insights into cardiovascular status 33 , 34 . They improve patient compliance by eliminating the discomfort of traditional methods and enhance medication adherence through real-time feedback. Monitoring both carotid arteries is advantageous, as it helps detect asymmetrical arterial stiffness and localized issues such as atherosclerosis, offering a more comprehensive assessment of cardiovascular health 24 , 25 . Furthermore, bilateral monitoring is used to identify significant blood pressure differences between the arteries, indicating severe conditions such as stenosis or aortic dissection 25 . This approach enhances patient safety and supports better treatment planning and postoperative care, particularly in high-risk patients and those undergoing carotid artery surgery. Two disposable snCMUT patches were conformally placed in contact on both sides of the necks of 9 healthy volunteers for simultaneous monitoring of the left and right carotid arteries (Supplementary Fig. 12). To locate the carotid artery, the disposable snCMUT patch was moved while observing and acquiring B-mode images (Supplementary videos 1 and 2). For the safety of the subjects, the bias voltage was limited to 30 V. The disposable snCMUT patches were operated under 24.5 V PP of 4.25 MHz pulse. The vertical cross-sections of the common carotid artery (CCA) on the right (Fig. 5 b) and left (Fig. 5 c) sides of the neck were monitored via B-mode ultrasound images. The enhanced transmission efficiency provided by silicon nanocolumns allowed for relatively clear ultrasound images compared to previously reported wearable ultrasound imagers 4 , 8 , enabling the distinction of CCA and the internal jugular vein (JV) and sternocleidomastoid muscle (SCM). In the transverse ultrasound images of the carotid artery, the outer wall of the CCA was relatively clearly identified (Fig. 5 d), and pulsations were monitored in real-time. Despite operating at a very low voltage, the disposable snCMUT patches obtained more precise ultrasound images than previously reported wearable ultrasound imagers that required voltage amplification up to 200 V by boost converters with high power consumption. The low operating voltage was remarkable and required for wearable ultrasound patches. The blood pressure of the carotid artery acquired from the disposable snCMUT patches attached to the human neck through M-mode ultrasound imaging allowed continuous monitoring of the pathophysiological status (Fig. 5 e). The contraction and expansion of the carotid artery were measured through the positional changes of the anterior and posterior walls, tracking the blood pressure changes over time. The changes in the distance between carotid artery walls were converted into blood pressure pulse waves and compared with a clinical-grade tonometer. The disposable snCMUT patch exhibited over 96.5% agreement with the clinical-grade tonometer (Fig. 5 f, Supplementary Fig. 13), even preventing potential operator-dependent recording errors using handheld probes, such as vessel squeezing or misalignment (Supplementary Fig. 14). In healthy volunteers, there was little difference in blood pressure readings obtained simultaneously from both sides of the neck using the disposable snCMUT patches (Fig. 5 g), as healthy individuals typically do not exhibit carotid artery asymmetry. Continuous monitoring of the carotid arteries in patients using disposable snCMUT patches can help detect asymmetrical arterial stiffness and localized conditions such as atherosclerosis, offering a more comprehensive assessment of cardiovascular health. For this study, the human carotid artery was continuously monitored using low-cost, ultra-thin, silicon-based disposable snCMUT, obtaining detailed blood pressure pulse waveforms, including the maximal slope, systolic peak, dicrotic notch, and diastolic peak. Conclusions In conclusion, developing disposable snCMUT patches marks a significant advancement in wearable ultrasound technology. The proposed patches offer a lead-free, flexible, and cost-effective solution for real-time medical imaging and monitoring. Using semiconductor fabrication on silicon wafers, snCMUTs present an eco-friendly alternative to traditional lead-based piezoelectric materials, significantly reducing manufacturing costs and making disposable probes a viable option. By incorporating silicon nanocolumns, the patches deliver high transmission efficiency and superior image resolution at low operational voltages, making them ideal for continuously monitoring critical parameters such as blood pressure and arterial health. Exceptional flexibility and thinness make snCMUT patches particularly suited for wearable applications, while their enhanced imaging performance and improved SNR have been validated in both phantom imaging and real-time carotid artery monitoring on both sides of the neck simultaneously. The innovation offers a promising approach to non-invasive cardiovascular health monitoring, providing valuable clinical insights with minimal discomfort and high diagnostic accuracy. Currently, the application of the low-cost, disposable snCMUT patches across various parts of the human body is under development to capture real-time three-dimensional ultrasound images, which is expected to enhance ultrasound imaging further and deliver crucial clinical insights. Methods Materials All chemicals used during the fabrication of CMUT, including acetone, isopropyl alcohol (IPA), buffered oxide etchant (BOE) 6:1, hydrogen peroxide, and sulfuric acid, were purchased from Daejung Chemicals, Inc. (South Korea). Highly doped silicon wafers (p-type, 100) were purchased from WaferBiz (South Korea). On the other hand, highly doped SOI wafers (p-type, 100) were purchased from BMIT (South Korea). In conventional photolithography, two photoresist (PR) types were used: positive and negative PR. The positive PRs used were AZ GXR-601 (46 cP) and AZ 10-XT (520 cP) from Merk KGaA (Germany). The negative PRs, namely DNR-L-300-40 (120 cP) and DNR-L-300-D1 (21.8 cP), were purchased from Dongjin Semichem Co., Ltd. (South Korea). Aluminium etchant APAL-1 was purchased from Labotech (South Korea). Polydimethylsiloxane (PDMS; SYLGARD 160) for the acoustic lens was purchased from Dow Corning (USA). Fabrication of silicon nanocolumns Four-inch bare silicon and SOI wafers were used to establish top-down fabrication of silicon nanocolumns at a single wafer level. The silicon and SOI wafers underwent a thermal treatment in an 1100 ºC furnace, resulting in the growth of a 200 nm silicon dioxide layer. Negative PR was applied to define silicon columns using conventional photolithography. Specifically, DNR-L300-D1 (21.8 cP) was spin-coated on the wafer. Following a baking step, UV light was exposed to the substrate using an EVG®6200 NT mask aligner (EV Group, Austria), and subsequent pre-exposure bake and development (AZ 300 MIF) processes were conducted. Furthermore, the silicon dioxide layer was etched using RIE via Plasmalab800Plus (Oxford Instruments, UK). DRIE was employed to define silicon columns at the micrometre scale using the Omega® LPX-DSi Etch system (SPTS Technologies Ltd., United Kingdom). A second round of RIE was applied to the micro-silicon columns to reduce their size through undercutting via horizontal etching, which smoothed the scallops from DRIE. Additionally, a systematic investigation was conducted on the anisotropy of the RIE process, and the etching conditions were fine-tuned to achieve precise fabrication of nano-sized silicon columns as springs. Evaluation of mechanical characteristics of silicon nanocolumns To evaluate the top-down fabricated silicon nanocolumns, in-situ nanomechanical characterizations were performed using a pico-indenter (Hysitron PI 85L PicoIndenter; Bruker, Corp., USA), which was integrated with the imaging capabilities of a scanning electron microscope (SEM) (Nova Nano SEM 450; FEI, Netherlands), allowing for simultaneous quantitative nanomechanical testing and imaging. A 10 µm diameter flat tip was employed to apply force to the nanocolumns, facilitating the assessment of their elastic deformation. To minimize buckling, the flat tip was positioned as perpendicular to the substrate as possible. For accurate characterization, the silicon nanocolumns were subjected to repeated compression by the pico-indenter across at least 10 individual samples, and the average displacement was recorded. Design and fabrication of snCMUT array To integrate silicon nanocolumns into CMUT as springs, FEA was conducted using the commercial tool COMSOL Multiphysics (COMSOL, Inc., USA). Nine silicon nanocolumns were arranged in a 3×3 grid within a single cell of the CMUT, and each cell was designed as a square with dimensions of 120×120 µm. A vacuum gap of 180 nm was designed for ultrasound generation. A single element in the CMUT array comprised 20 cells arranged in a 2-row and 10-column configuration. A piston structure (stiff block) was positioned above each cell to prevent the bending of the moving plate. The thickness of the moving plate was set at 2 µm, and the 64-element CMUT array was designed with a resonance frequency of 5 MHz for ultrasound imaging of the human carotid artery vessels. The fabrication involved wafer bonding of two SOI wafers, which had undergone DRIE and chemical mechanical polishing (CMP) to ensure precise dimensional control during fabrication. A SOI wafer with a device layer electrical resistivity of < 0.005 ohm-cm was selected to establish bottom and top plate electrodes. Fabrication details are provided in Supplementary Fig. 4. Initially, a silicon dioxide layer was grown and patterned on the SOI wafer using conventional photolithography techniques (EVG®6200 NT mask aligner, EV Group, Austria). Subsequent oxidation resulted in a vacuum gap height of 180 nm. The CMUT element and the lateral dimensions of the silicon nanocolumns were defined through DRIE using the Omega® LPX-DSi Etch system (SPTS Technologies Ltd., United Kingdom) after the silicon dioxide layer was etched using RIE via Plasmalab800Plus (Oxford Instruments, UK). The top-side SOI wafer was roughly aligned with the processed bottom SOI wafer, and wafer bonding was accomplished in a high-vacuum environment using the SB8 GEN2 system (Suss Microtec, Germany). The handle layer of the top SOI wafer was then grounded and polished to obtain 20 µm-thick piston top plates. A silicon dioxide mask was grown, accurately defining the piston structures and defining the top plate using DRIE. To establish electrical contact for the bottom and top plate electrodes, the layers including the buried oxide (BOX) layer, the device layer of the top SOI wafer, and the silicon dioxide layer in the bottom SOI wafer, were sequentially etched. The process culminated with the deposition and patterning of a 500 nm aluminum layer to create electrical contact pads. Each CMUT element was singulated via frontside and backside trenches formed by conventional photolithography and DRIE. The trenches were filled with PDMS to produce flexible and stretchable snCMUTs. Following fabrication, the processed SOI wafer was diced via blade dicing to segment the arrays of snCMUTs. Characterization of snCMUT array For evaluating the fabricated snCMUT array, SEM examinations were conducted using the Nova Nano SEM 450 (FEI, Netherlands). To carefully assess the shape of the silicon nanocolumns within the snCMUTs, focused ion beam (FIB) cutting was employed using NX 5000 (Hitachi, Japan). This process enabled precise vertical sectioning of the silicon nanocolumns, facilitating SEM observations to measure their shapes and dimensions. Additionally, the establishment of the vacuum gap and bonding of the moving plate were verified. To delve into the operational characteristics of the fabricated snCMUTs, an impedance analyser (4294A, Agilent Technologies Inc., USA) was used to confirm the phase and amplitude of the electrical input impedance, thereby assessing the electrical properties. A DC bias was applied to the snCMUT array via a bias T circuit and a high-voltage power supply, with a small-signal AC voltage of 100 mV superimposed (Supplementary Fig. 15). Calibration was meticulously conducted to invalidate potential parasitic influences. To analyze the mechanical movement of the moving plate of snCMUTs, an LDV integrated with a microscope (OFV-2570; Polytec GmbH, Germany) was employed (Supplementary Fig. 16). Measurement of the acoustic pressure generated by the snCMUT was conducted using a hydrophone (HGL-0200; Onda Co., USA) coupled with a pre-amplifier (Supplementary Fig. 17). The hydrophone signals were read out through a digitizing oscilloscope (Infiniium 54825A; Agilent Technologies Inc., USA). To simultaneously apply DC and AC voltages, a bias T circuit was used, with the DC voltage sourced from a high-voltage power supply (SRS PS310; Stanford Research System, USA) and the AC voltage applied through the AC function generator (Agilent 33500B; Agilent Technologies Inc., USA). The FBW of the transmitted ultrasound waves from the snCMUT array was calculated by acquiring the corresponding hydrophone signals. Conversely, the FBW of the pulse-echo signal from snCMUT, reflected from the metal bar in FC-40 immersed condition, was calculated by acquiring signals using an ultrasound pulser/receiver (5073PR, Olympus, Inc., USA). Packaging and prototyping disposable snCMUT patch To produce snCMUT for disposable patches, an in-house-designed FPCB was fabricated and employed. An adhesive was used to demonstrate the wearable CMUT's capability for ultrasound imaging to mount the fabricated snCMUT array. The FPCB was designed to include connectors for a commercial ultrasound imaging system (Vantage 256 research ultrasound system; Verasonics Inc., USA). Pre-tests were conducted using wire-bonding and anisotropic conductive film (ACF) for the electrical connection between snCMUT and FPCB. Since no significant differences were observed between the two methods, both methods were used for packaging. After electrical connections, 100 nm of parylene C was deposited via a parylene coating system (LAVIDA 110; FemtoScience, South Korea). The entire assembly was encapsulated with a PDMS lens for insulation and ultrasound focusing. Acoustic characterizations using commercial standard phantom In this study, the conventional CMUT probe selected for comparison with snCMUT was fabricated using double oxidation and standard wafer bonding process 35 – 37 , comprising a 192 channels linear array. Only 64 channels were operated for acoustic characterizations, the same as the number of channels in the snCMUT array. Imaging performance evaluation of both conventional CMUT probe and disposable snCMUT patch was conducted using standard ultrasound imaging phantom (ATS-539, CIRS) for dead zone group (2–10 mm, with 1 mm spacing), vertical group (10–180 mm, with 10 mm spacing), axial-lateral resolution array (40, 70, 110, and 160 mm, with 4, 3, 2, 1, 0.5, and 0.25 mm spacing) and anechoic target structures (10–170 mm, with 20 mm spacing). Transducers were vertically placed on the surface of the phantom using ultrasound gel and operated using a commercial ultrasound imaging system (Vantage 256 system, Verasonics, Inc.) to scan over the phantom and reconstruct the imaging planes by employing a plane-wave compounding method, custom-programmed by MATLAB (MathWorks). Pulse-echo intensity, axial/lateral full width half maximum (FWHM) based on the point spread function (PSF) of the target, and ultrasound image contrast were analysed from the ultrasound images of the phantom targets acquired by both the conventional CMUT probe and disposable snCMUT patch. Wearable B-mode imaging on the human body The bio-interface excursions, carotid artery, and blood pressure waveforms were measured in healthy participants. All human tests were approved by the Seoul National University College of Medicine and Seoul National University Hospital, Institutional Review Board protocol (H-2007-145-1143). The participants provided their voluntary consent for ultrasonic measurements. Using the disposable snCMUT patches, real-time B-mode imaging on the human body was performed on nine healthy participants with relatively low and stable bias voltage for CMUT (30 V) provided by a commercial DC supply (PS310, Stanford Research Systems) and a transmitting voltage (24.5 V PP ) provided by a commercial multi-channel controller, which was within the safe range of intensity defined by the Food and Drug Administration (< 190 W/cm 2 ) to ensure the safety of the subject. Disposable snCMUT patches were attached to the skin surface using commercial medical tape (3M Soft Cloth Tape with Liner 2762, 3M Company), manually aligned to the cross-sectional area of the CCA through real-time B-mode imaging. High-quality images of the CCA and adjacent JV and SCM were acquired using disposable snCMUT patches operated by the commercial multi-channel controller with a custom-programmed plane-wave compounding reconstruction algorithm. Measurement and validation of blood pressure waveforms on carotid artery Based on the real-time B-mode imaging acquired by the disposable snCMUT patch, a single channel of the patch facing the top surface of the anterior wall of the CCA was manually selected for operation using M-mode imaging, specifically for measuring the CCA blood vessel diameter change over time acquired from pulse-echo signals from the anterior and posterior walls of the CCA. Using the wall tracking method, blood vessel diameter information was acquired and filtered to calculate the blood pressure waveforms of CCA. To validate the ability of the disposable snCMUT patches to measure blood pressure waveforms, the commercial pressure sensor-based blood pressure waveform analyser (DMP-1000 PLUS, DAEYOMEDI Co., Ltd., South Korea) was used to acquire blood pressure waveforms of CCA at the exact position of the same subject in close succession after the measurement using the disposable snCMUT patch. Systolic and diastolic blood pressure values from a commercial blood pressure cuff (JPN616T, OMRON Corporation, Japan) were additionally provided for calculating actual blood pressure for both disposable snCMUT patch and commercial pressure sensor. Declarations Data availability All data supporting the findings and conclusions of this study are available within the paper and its Supplementary Information files. All other relevant data are available from the corresponding author upon request. Source data are provided in this paper. Acknowledgments This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (RS-2024-00341714), the internal program of the Korea Institute of Science and Technology (2E33141, 2E3312H), and the Korea Medical Device Development Fund grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health & Welfare, the Ministry of Food and Drug Safety) (RS-2023-00254676). All bio-experiments were conducted in accordance with the approval of the Institutional Review Board of the Seoul National University College of Medicine and Seoul National University Hospital. Author contributions D.-H.K., SC, BT K.-Y., and BCL conceived and designed the research. HYK and BCL performed the simulations. D.-H.K., SC, HYK, SS, and BCL designed and performed all the fabrication and device measurements. D.-H.K., S.C., D.H.K, B.J., Y.S.L., E.-A.P., W.L., and B.C.L. performed all the clinical studies. D.-H.K., S.C., E.-A.P., W.L., H.K., M.I., J.-W.J., and B.C.L. analysed the data. J.-W.J. and BCL supervised the research and are project administrators. D.-H.K. and SC initially wrote the paper. All authors provided active and valuable discussions and feedback on the manuscript. Competing interests The authors declare no competing interests. References La T, Le LH (2022) Flexible and Wearable Ultrasound Device for Medical Applications: A Review on Materials, Structural Designs, and Current Challenges. Adv Mater Technol 7:2100798 Brenner K et al (2019) Advances in Capacitive Micromachined Ultrasonic Transducers. Micromachines (Basel) 10:152 Khuri-Yakub BT, Oralkan Ö (2011) Capacitive micromachined ultrasonic transducers for medical imaging and therapy. J Micromech Microeng 21:054004 Hu H et al (2023) A wearable cardiac ultrasound imager. 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Nano Energy 114:108636 Murray CSG, Nahar T, Kalashyan H, Becher H, Nanda NC (2018) Ultrasound assessment of carotid arteries: Current concepts, methodologies, diagnostic criteria, and technological advancements. Echocardiography 35:2079–2091 Kenny J-ÉS et al (2021) A novel, hands-free ultrasound patch for continuous monitoring of quantitative Doppler in the carotid artery. Sci Rep 11:7780 Bhuyan A et al (2011) Miniaturized, wearable, ultrasound probe for on-demand ultrasound screening. in. IEEE International Ultrasonics Symposium 1060–1063 (IEEE, 2011). 10.1109/ULTSYM.2011.0260 Apte N, Vaithilingam S, Sarioglu AF, Kupnik M, Khuri-Yakub BT (2011) Large area 1D CMUT phased arrays for multi-modality ultrasound imaging. in IEEE International Ultrasonics Symposium 612–615 (IEEE, 2011). 10.1109/ULTSYM.2011.0148 Park KK, Oralkan O, Khuri-Yakub BT (2013) A comparison between conventional and collapse-mode capacitive micromachined ultrasonic transducers in 10-MHz 1-D arrays. IEEE Trans Ultrason Ferroelectr Freq Control 60:1245–1255 Additional Declarations There is NO Competing Interest. Supplementary Files SupplementaryInformationforNatElectronV11.docx Supplementary Information SupplementaryVideo1notitle.mp4 Supplementary Video 1 SupplementaryVideo2notitle.mp4 Supplementary Video 2 Cite Share Download PDF Status: Published Journal Publication published 18 Jul, 2025 Read the published version in Nature Communications → Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5309768","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":378909413,"identity":"0199f32f-ba25-46e2-844b-15e8edd19d2a","order_by":0,"name":"Byung Chul 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Technology","correspondingAuthor":false,"prefix":"","firstName":"Jae-Woong","middleName":"","lastName":"Jeong","suffix":""}],"badges":[],"createdAt":"2024-10-22 08:20:45","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-5309768/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-5309768/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1038/s41467-025-61903-x","type":"published","date":"2025-07-18T04:00:00+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":73869694,"identity":"b7037ba6-ade1-4c30-8fd7-55c3f01f67e2","added_by":"auto","created_at":"2025-01-15 12:09:45","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":651267,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cstrong\u003eOverview of the disposable snCMUT patches.\u003c/strong\u003e \u003cstrong\u003ea\u003c/strong\u003e, Schematic illustrations of disposable snCMUT patches for continuous monitoring of both sides of the human carotid arteries. The disposable snCMUT patches are lead-free and avoid environmental restrictions. \u003cstrong\u003eb\u003c/strong\u003e, Schematic illustrations of single snCMUT patch including PDMS lens, snCMUT array, and in-house designed FPCB. \u003cstrong\u003ec\u003c/strong\u003e, Schematic illustrations of 4-cells of snCMUT and driving mechanism of substrate-embedded silicon nanocolumns as springs. \u003cstrong\u003ed-e\u003c/strong\u003e, Optical images of the mechanical compliance of the snCMUT array when bent with a tweezer (\u003cstrong\u003ed\u003c/strong\u003e) and the disposable snCMUT patch when twisted (\u003cstrong\u003ee\u003c/strong\u003e). \u003cstrong\u003ef\u003c/strong\u003e, B-mode imaging of the common carotid artery (CCA), jugular vein (JV), and sternocleidomastoid muscle (SCM) while the subject is rested (left). M-mode imaging of the pulsation pattern of CCA walls (right).\u003c/p\u003e","description":"","filename":"image1.png","url":"https://assets-eu.researchsquare.com/files/rs-5309768/v1/83074268f71ecbd807fd0fb8.png"},{"id":73869697,"identity":"929e4bd8-7ec3-4692-8ea6-5fec0270ab3a","added_by":"auto","created_at":"2025-01-15 12:09:45","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":924826,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cstrong\u003eFabrication of silicon nanocolumn and snCMUT arrays.\u003c/strong\u003e \u003cstrong\u003ea-b\u003c/strong\u003e, The FEA simulation of moving plate in CMUT compared to conventional CMUT (\u003cstrong\u003ea\u003c/strong\u003e) and snCMUT (\u003cstrong\u003eb\u003c/strong\u003e). The snCMUT achieves a high average displacement through the parallel motion of the plate induced by nano-silicon springs. \u003cstrong\u003ec\u003c/strong\u003e, Schematic illustrations of fabrication steps for top-down approaches of silicon nanocolumn: i) PR patterning by conventional photolithography on a 4-inch silicon wafer after oxidation for growth silicon dioxide, ii) dry etching of silicon dioxide layer by RIE, iii) Defining silicon microcolumn via DRIE, iv) shrink down of silicon microcolumn via RIE to produce silicon nanocolumn. \u003cstrong\u003ed-e\u003c/strong\u003e, SEM images of fabricated silicon microcolumn (\u003cstrong\u003ed\u003c/strong\u003e) and silicon nanocolumns with diameters of 800 nm (\u003cstrong\u003ee-i\u003c/strong\u003e), 600 nm (\u003cstrong\u003ee-ii\u003c/strong\u003e), and 300 nm (\u003cstrong\u003ee-iii\u003c/strong\u003e). Etch time and opening area (\u003cem\u003eW\u003c/em\u003e) of RIE in e-i, ii, and iii are 5, 6, and 8 min, 10, 5, and 4 µm, respectively. The scale bar is 2 µm. \u003cstrong\u003ef\u003c/strong\u003e, The diameter of the silicon nanocolumn as the etch time and opening area. \u003cstrong\u003eg\u003c/strong\u003e, SEM image of silicon nanocolumn while pico-indentation (left) and the compressed displacement of silicon nanocolumns as pico-indentation force and diameters of silicon nanocolumns (right). The scale bar is 2 µm. \u003cstrong\u003eh\u003c/strong\u003e, photograph of fabricated snCMUT array. \u003cstrong\u003ei-k\u003c/strong\u003e, SEM images of fabricated snCMUT. Perspective view of snCMUT (\u003cstrong\u003ei\u003c/strong\u003e). The piston top plate was cut via FIB to evaluate the dimension of the silicon nanocolumn and the vacuum gap. The cross-sectional SEM image of the silicon nanocolumn (\u003cstrong\u003ej\u003c/strong\u003e) and the 180 nm vacuum gap (\u003cstrong\u003ek\u003c/strong\u003e). During the cutting of the piston top plate via FIB, by-products from ion milling were redeposited onto the silicon nanocolumn. The scale bar is 50 µm in i, 1 µm in j, and 100 nm in k. \u003cstrong\u003el-m\u003c/strong\u003e, TEM images of wafer bonding surface (\u003cstrong\u003el\u003c/strong\u003e) and thermally grown silicon dioxide surface (\u003cstrong\u003em\u003c/strong\u003e). Despite the narrow cross-sectional area of the silicon nanocolumn, it was well bonded with the top SOI wafer. The scale bar is 5 nm.\u003c/p\u003e","description":"","filename":"image2.png","url":"https://assets-eu.researchsquare.com/files/rs-5309768/v1/826c33116c8497222a20d232.png"},{"id":73870403,"identity":"110baf4a-dc01-4f0e-bcb6-4fc6881280f7","added_by":"auto","created_at":"2025-01-15 12:17:45","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":481551,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cstrong\u003eCharacterization of snCMUT.\u003c/strong\u003e \u003cstrong\u003ea-b\u003c/strong\u003e, Electrical impedance measurements of the corresponding element of the snCMUT: the phase part (\u003cstrong\u003ea\u003c/strong\u003e) and the amplitude part (\u003cstrong\u003eb\u003c/strong\u003e). The electrical impedance was measured using different bias voltages, 10 to 50 V of DC. Phase shifts with bias voltage were observed. The resonant frequency in air was 6.7 MHz at 50 V of bias. \u003cstrong\u003ec\u003c/strong\u003e, The dynamic plate displacement of the snCMUT measuring from a laser Doppler vibrometer. The maximum displacement was measured in the four-piston top plate's positive (left) and negative (right) direction. \u003cstrong\u003ed\u003c/strong\u003e, The maximum peak-to-peak displacement of the four-piston top plate. \u003cstrong\u003ee\u003c/strong\u003e, Comparison of the displacement profile of the moving top plate between conventional CMUT and snCMUT. The measured displacement profile is from the red dashed line in Figure 3d. Even though there are variations in the maximum displacement of each piston top plate, the embedded silicon nanocolumn induces parallel motion of the top plate in CMUT, which causes enhanced average displacement. \u003cstrong\u003ef-g\u003c/strong\u003e, Measured acoustic pressure of the snCMUT via hydrophone as follows a number of operating elements at a distance of 3.5 mm in corn oil: transient pressure (\u003cstrong\u003ef\u003c/strong\u003e) and its frequency spectrum (\u003cstrong\u003eg\u003c/strong\u003e). \u003cstrong\u003eh\u003c/strong\u003e, The pulse-echo response and frequency spectrum of a representative array element.\u003c/p\u003e","description":"","filename":"image3.png","url":"https://assets-eu.researchsquare.com/files/rs-5309768/v1/3a550e8b98deaf27f1db4f74.png"},{"id":73869696,"identity":"7b181f95-95ba-4a86-b748-17aa657d3ef9","added_by":"auto","created_at":"2025-01-15 12:09:45","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":889761,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cstrong\u003eUltrasound images of the commercial phantom and their evaluation.\u003c/strong\u003e \u003cstrong\u003ea-b\u003c/strong\u003e, The optical photograph of the conventional CMUT probe (\u003cstrong\u003ea\u003c/strong\u003e) and the disposable snCMUT patch (\u003cstrong\u003eb\u003c/strong\u003e) on the top surface of the commercial phantom with ultrasound gel. The blue and red boxes indicate the visible area of the phantom by conventional CMUT and disposable snCMUT patches, respectively. \u003cstrong\u003ec\u003c/strong\u003e, B-mode images of the conventional CMUT probe in the vertical group and anechoic target of the commercial phantom. The conventional CMUT was operated with a DC bias voltage of 70 V, complemented by 30.6 V\u003csub\u003ePP\u003c/sub\u003e and 6.25 MHz of AC wave. \u003cstrong\u003ed-e\u003c/strong\u003e, B-mode images of the disposable snCMUT patch in the vertical group and anechoic target (\u003cstrong\u003ed\u003c/strong\u003e) and the axial-lateral resolution array (\u003cstrong\u003ee\u003c/strong\u003e) of the commercial phantom. The snCMUT was operated with a DC bias voltage of 40 V, complemented by 8.9 V\u003csub\u003ePP\u003c/sub\u003e and 4.25 MHz of AC wave. \u003cstrong\u003ef\u003c/strong\u003e, Intensity of vertical group targets as a function of the imaging depth of conventional CMUT and snCMUT. \u003cstrong\u003eg-h\u003c/strong\u003e, Axial (\u003cstrong\u003eg\u003c/strong\u003e), and lateral (\u003cstrong\u003eh\u003c/strong\u003e) resolutions of the vertical group targets along the imaging depth compared between PZT-based commercial ultrasound probe, conventional CMUT, and snCMUT. \u003cstrong\u003ei\u003c/strong\u003e, Contrast of B-mode images as a function of the imaging depth of conventional CMUT and snCMUT. Compared to the conventional CMUT probe, the disposable snCMUT patch offers enhanced contrast and the ability to image deeper regions, thanks to its high transmission efficiency.\u003c/p\u003e","description":"","filename":"image4.png","url":"https://assets-eu.researchsquare.com/files/rs-5309768/v1/130d6271066c950a550a6abc.png"},{"id":73869698,"identity":"16247a5e-095a-41c6-86a1-78bdfe182922","added_by":"auto","created_at":"2025-01-15 12:09:45","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":780465,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cstrong\u003eHuman carotid artery ultrasound images and monitoring of blood pressure obtained with prototype disposable ultrasound patches.\u003c/strong\u003e \u003cstrong\u003ea\u003c/strong\u003e, Photograph of attached disposable snCMUT patches onto both sides of the human neck for real-time ultrasound imaging and blood pressure monitoring of human carotid arteries. \u003cstrong\u003eb-c\u003c/strong\u003e, The B-mode images at the human neck surface aligned with vertical cross-sections of the common carotid artery (CCA) on the right (\u003cstrong\u003eb\u003c/strong\u003e) and left (\u003cstrong\u003ec\u003c/strong\u003e) side of the neck. The enhanced transmission efficiency provided clear ultrasound images, enabling the distinction of not only the CCA but also the internal jugular vein (JV) and sternocleidomastoid muscle (SCM). \u003cstrong\u003ed\u003c/strong\u003e, The B-mode image of left neck side transverse CCA for monitoring carotid artery pulsations from detecting wall. The applied DC bias voltage was 30 V supplemented by 24.5 V\u003csub\u003ePP\u003c/sub\u003e of 4.25 MHz AC wave for ultrasound imaging via disposable snCMUT patches. \u003cstrong\u003ee\u003c/strong\u003e, M-mode image of the pulsation pattern of CCA walls. \u003cstrong\u003ef\u003c/strong\u003e, The waveforms of blood pressure derived from carotid vessel diameters of the M-mode image (Fig. 5e) as a function of time. Physiological parameters from the arterial pulse waveforms, including the heart rate and blood pressure, were derived. \u003cstrong\u003eg\u003c/strong\u003e, Systolic blood pressure on the left and right side of the neck of 9 healthy volunteers simultaneously monitored by two disposable snCMUT patches.\u003c/p\u003e","description":"","filename":"image5.png","url":"https://assets-eu.researchsquare.com/files/rs-5309768/v1/d5269cd2da08568a2e2e621d.png"},{"id":88505753,"identity":"d5810873-f5ad-4705-8f34-fde0777bc8b4","added_by":"auto","created_at":"2025-08-07 07:29:09","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":5242194,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-5309768/v1/b875a62d-142f-41d7-b1a6-661d5e96fca7.pdf"},{"id":73869700,"identity":"52b94bad-b8a2-4c28-9df0-d3d5edeb1a0a","added_by":"auto","created_at":"2025-01-15 12:09:46","extension":"docx","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":26642773,"visible":true,"origin":"","legend":"Supplementary Information","description":"","filename":"SupplementaryInformationforNatElectronV11.docx","url":"https://assets-eu.researchsquare.com/files/rs-5309768/v1/a61601cf1ca4197038ef036b.docx"},{"id":73869701,"identity":"70615f47-086f-447b-b80f-046492bd036a","added_by":"auto","created_at":"2025-01-15 12:09:48","extension":"mp4","order_by":2,"title":"","display":"","copyAsset":false,"role":"supplement","size":126926625,"visible":true,"origin":"","legend":"Supplementary Video 1","description":"","filename":"SupplementaryVideo1notitle.mp4","url":"https://assets-eu.researchsquare.com/files/rs-5309768/v1/9628ee1bd3a897a87495dae3.mp4"},{"id":73869742,"identity":"8a3e8f06-877f-4a16-80a1-f478c0f7c5a3","added_by":"auto","created_at":"2025-01-15 12:09:49","extension":"mp4","order_by":3,"title":"","display":"","copyAsset":false,"role":"supplement","size":166070529,"visible":true,"origin":"","legend":"Supplementary Video 2","description":"","filename":"SupplementaryVideo2notitle.mp4","url":"https://assets-eu.researchsquare.com/files/rs-5309768/v1/58b3ca139bdfbcd9317f82cc.mp4"}],"financialInterests":"There is \u003cb\u003eNO\u003c/b\u003e Competing Interest.","formattedTitle":"Silicon nanocolumn-based disposable and flexible ultrasound patches","fulltext":[{"header":"Main","content":"\u003cp\u003eUltrasound is a widely used medical imaging modality, providing non-invasive and safe real-time imaging of deep-lying tissues, organs, and blood flow\u003csup\u003e\u003cspan additionalcitationids=\"CR2\" citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e\u003c/sup\u003e. Compared to X-ray, computed tomography (CT), and magnetic resonance imaging (MRI), ultrasound machines are relatively compact and inexpensive\u003csup\u003e\u003cspan additionalcitationids=\"CR4\" citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e\u003c/sup\u003e. Recent advancements in wearable devices and technology have led to the development of wearable ultrasonic transducer arrays\u003csup\u003e\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e,\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e,\u003cspan additionalcitationids=\"CR7 CR8 CR9 CR10 CR11 CR12 CR13 CR14\" citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e\u003c/sup\u003e, which enable various applications, including monitoring blood flow in different body regions, imaging cardiovascular structures, and mapping tissue moduli.\u003c/p\u003e \u003cp\u003eHowever, wearable ultrasound devices pose issues related to their inherent material limitations. First, as the primary component of wearable ultrasound devices, piezoelectric materials predominantly containing lead pose environmental issues and violate the Restriction of Hazardous Substances Directive (RoHS). Second, the inherent properties of piezoelectric materials necessitate thick backing layers to define ultrasound propagation direction and minimize pulse duration while quickly damping vibrations\u003csup\u003e\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e,\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e\u003c/sup\u003e. When backing layers are insufficient, both axial and lateral resolution worsen due to the ringing and reverberation of transducers. The thick layers make them unsuitable for wearable ultrasound technology, especially wearable patches for continuous monitoring, as they introduce discomfort when attached to the body. Third, assembling rigid piezoelectric materials with flexible electrodes to achieve conformal contact becomes increasingly difficult as array size and density increase due to fabrication based on blade dicing\u003csup\u003e\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e,\u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e\u003c/sup\u003e. Thus, obstacles arise in medical imaging fields requiring narrow pitch, minimized kerf, and numerous elements to achieve high-resolution imaging attributable to the transducer beam width. Last, the high production cost of ultrasound transducer arrays typically necessitates the reuse of wearable patch-type ultrasound probes, driven by economic challenges\u003csup\u003e\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e,\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e\u003c/sup\u003e. While the reuse of ultrasound probes with disposable medical cover is standard in medical imaging, primarily due to the high production cost and environmental issues associated with lead-based piezoelectric materials, the concept of disposable ultrasound probes offers compelling advantages to wearable patches. Disposable ultrasound probes address hygiene, infection control, and cross-contamination issues in medical environments, thereby alleviating the burden of cleaning and sterilization between uses and offering convenience and cost-effectiveness to healthcare providers and patients. Particularly, disposable ultrasound probes enhance patient safety and eliminate the need for sterilization to streamline diagnostic procedures in environments following stringent hygiene protocols.\u003c/p\u003e \u003cp\u003eBased on silicon wafers and semiconductor fabrication technology, capacitive micromachined ultrasonic transducers (CMUTs) offer a lead-free alternative to piezoelectric material-based ultrasound transducers with considerable potential for medical imaging, as they boast high element density and enable miniaturization advantages\u003csup\u003e \u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e,\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e,\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e \u003c/sup\u003e. Free from lead, CMUTs circumvent environmental regulations, while batch production capabilities drastically reduce manufacturing costs even with high element density (under \u003cspan\u003e$\u003c/span\u003e12, Supplementary Table\u0026nbsp;1), facilitating the realization of disposable ultrasound probe patches. Moreover, CMUTs offer superior sensitivity, image resolution, and bandwidth compared to conventional piezoelectric material-based ultrasound probes\u003csup\u003e \u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e,\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e,\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e \u003c/sup\u003e. Unlike piezoelectric transducers that experience degraded piezoelectric characteristics and signal-to-noise ratio (SNR) due to their rising temperature during long-term use, CMUTs avoid temperature increases even during extended use, making them more suitable for wearable patches intended for long-term monitoring\u003csup\u003e \u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e \u003c/sup\u003e. However, despite the potential of CMUTs for high-resolution ultrasound imaging in wearable patches, their adoption faces challenges, including low output pressure and high bias voltage, which limit imaging depth and elevate driving voltage\u003csup\u003e \u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e,\u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e23\u003c/span\u003e \u003c/sup\u003e.\u003c/p\u003e \u003cp\u003eThis study presents a flexible CMUT with exceptional pressure output, designed for real-time wearable ultrasound imaging in disposable patches (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003ea). Leveraging semiconductor and micromachining technologies, the designed lead-free ultrasound patches based on silicon nanocolumn capacitive micromachined ultrasonic transducer (snCMUT) array are disposable and environmentally friendly, with minimized fabrication costs of under \u003cspan\u003e$\u003c/span\u003e20 (Supplementary Table\u0026nbsp;1). The skin-conformal ultrasound patch comprises individual flexible CMUT elements separated by elastomer-filled trenches, mounted on an in-house designed flexible printed circuit board (FPCB), and encapsulated with polydimethylsiloxane (PDMS) (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003eb). To enhance transmission efficiency and reduce power consumption, silicon nanocolumns are utilized as springs beneath the moving plate of snCMUT, increasing the average displacement (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003ec). Fabricated using a rigid-island technique, the snCMUT array boasts high flexibility (minimum radius of curvature: \u0026lt;1 mm), stretchability (up to 140% without degradation), and minimized thickness (~\u0026thinsp;900 \u0026micro;m fully packaged), making it ideal for wearable applications (Figs.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003ed and \u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003ee). To demonstrate the capabilities of disposable snCMUT patches, the human carotid artery is monitored on both sides of the neck in real-time. Owing to its elevated transmission efficiency, disposable snCMUT patches produce clear ultrasound images (B-mode for sonography and M-mode for blood pressure wave, Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003ef) even at low driving voltages (8.9 V\u003csub\u003ePP\u003c/sub\u003e, Supplementary Tabel 2). The cost-effective disposable snCMUT patches enable multi-point monitoring of blood pressure waveforms, offering significant clinical advantages for cardiovascular health assessment and management. By monitoring both carotid arteries simultaneously, disposable snCMUT patches assist in identifying hypertensive patients missed with single-side measurements, detecting asymmetrical arterial stiffness, guiding precise treatment plans, enhancing patient safety, and supporting surgical planning and postoperative evaluation\u003csup\u003e \u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e,\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e \u003c/sup\u003e.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e\n\u003ch3\u003eFabrication of flexible snCMUT arrays\u003c/h3\u003e\n\u003cp\u003eThe proposed snCMUT operates on a principle different from that of conventional CMUTs, which rely on the deformation of a thin plate\u003csup\u003e\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e,\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e\u003c/sup\u003e. In the design, snCMUTs use a thin plate with pistons driven by silicon nanocolumns as a spring\u003csup\u003e\u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e,\u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e27\u003c/span\u003e\u003c/sup\u003e. The structure introduces a spring constant in the mass\u0026ndash;spring\u0026ndash;damper system, enhancing average displacement and packing density through parallel piston movement and large unit cells, respectively (Figs.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003ea and \u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003eb, left). Finite element analysis (FEA) simulations provide design parameters of the CMUT structure with silicon nanocolumns, aiming for a resonant frequency of 5 MHz for carotid artery imaging (Figs.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003ea and \u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003eb, right). Conventional CMUTs offer a pull-in voltage of 93 V, while snCMUT exhibits the pull-in voltage of 105 V. Below the pull-in voltage, snCMUT shows higher displacement and more efficient output pressure due to a larger unit cell area. The design includes 20 piston top plates forming a CMUT element, each affixed with nine 2 \u0026micro;m long silicon nanocolumns. To integrate vertically oriented silicon nanocolumns into CMUTs, precise fabrication with specific dimensions is necessary. Thus, compatibility with CMUT fabrication is crucial, favouring a top-down approach via silicon wafer etching over a bottom-up approach.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eUsing deep reactive ion etching (DRIE) and reactive ion etching (RIE), silicon nanocolumns were fabricated with fixed central diameters of 1.5 \u0026micro;m and varying doughnut-shaped widths to control etch rates (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003ec). Silicon microcolumns of 1.45 \u0026micro;m diameter and scalloped sidewalls were fabricated using DRIE (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003ed, Supplementary Fig.\u0026nbsp;1a). While fabricating nanocolumns by reducing the silicon microcolumn size through RIE undercutting, precise control of the vertical and horizontal etch rate ( 984 and 758 \u0026Aring;/min, respectively, for this study) was crucial. The uniformity of etching anisotropy was confirmed even with increased etching time, and the undercut was controlled by varying the opening area (W) and etch time (Supplementary Fig.\u0026nbsp;2). Successful top-down fabrication of silicon nanocolumns (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003ee, Supplementary Fig.\u0026nbsp;1b-d) with diameters controlled by etch time was achieved (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003ef). The mechanical properties of the silicon nanocolumns, acting as springs, were evaluated using a pico-indenter. Smaller diameter nanocolumns contracted more, with 300 nm diameter nanocolumns changing length by 148 nm under 500 \u0026micro;N (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003eg). The uniaxial compressive strain recorded was 9.1%. Silicon nanocolumns with diameters below 100 nm were unstable, leading to the collapse of the top SiO\u003csub\u003e2\u003c/sub\u003e layer (Supplementary Fig.\u0026nbsp;3a). Assuming diameters of 500\u0026ndash;700 nm to be optimal for stability and mechanical performance in snCMUT, fabrication was equally well-defined in silicon-on-insulator (SOI) wafers with high repeatability (Supplementary Fig.\u0026nbsp;3b).\u003c/p\u003e \u003cp\u003eThe snCMUT array was fabricated via wafer bonding of two SOI wafers using conventional microfabrication technologies (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003eh, Supplementary Fig.\u0026nbsp;4). For wearable applications, PDMS was filled into trenches between elements singulated on both sides during DRIE for different trench widths to maximize flexibility (Supplementary Fig.\u0026nbsp;5). The cross-sectional cutting plane images obtained via a focused ion beam (FIB) confirmed the well-bonded silicon nanocolumns (Figs.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003ei\u0026ndash;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003ej) and formed vacuum gaps (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003ek). Transmission electron microscope (TEM) images of the wafer bonding interface (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003el) and the interface between SiO\u003csub\u003e2\u003c/sub\u003e grown by thermal oxidation and Si (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003em) confirmed the successful bonding of silicon nanocolumns and the Si plate of CMUT via wafer fusion bonding of two SOI wafers. The snCMUT array demonstrated impressive flexibility and stretchability, achieving bending with a radius of curvature less than 1 mm and elongation up to 140%, stretching from 40 mm to over 55 mm (Supplementary Fig.\u0026nbsp;6). The obtained high degree of stretchability prevented wearable snCMUT patch degradation, as the snCMUT array integrated into the FPCB avoided strain mismatch from tensile and compressive forces during bending.\u003c/p\u003e \u003cdiv id=\"Sec3\" class=\"Section2\"\u003e \u003ch2\u003eCharacterization of snCMUT\u003c/h2\u003e \u003cp\u003eTo evaluate the characteristics of the fabricated snCMUT array, a comprehensive assessment of amplitude and phase components of the electrical input impedance was performed using an impedance analyser. Despite slight changes in the phase and amplitude of the electrical input impedance, respectively, the fabricated snCMUT exhibited sharp changes in both phase and amplitude at the resonant frequency, as analysed using DC voltage increasing from 10 to 50 V (Figs.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003ea and \u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003eb). Since the silicon nanocolumns functioned as springs, the shift in impedance phase with increasing bias voltage in snCMUTs was minimized compared to conventional CMUTs, which are affected by static deflections\u003csup\u003e\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e,\u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e\u003c/sup\u003e. An average resonant frequency of 6.7 MHz was observed in air at 50 V.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eThe dynamic response of the snCMUT elements was evaluated using a laser Doppler vibrometer (LDV) integrated with a custom-built microscope. With a DC bias voltage of 50 V and a continuous wave pulse of 1 VPP at 4.7 MHz, LDV recorded the piston movement of the top plate along the z-axis (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003ec). Maximum displacements of 3.20 and \u0026minus;\u0026thinsp;3.64 nm were recorded in the positive and negative z-axis directions, respectively, indicating the balanced movement of the piston top plate. The balanced movement implied well-fabricated silicon nanocolumns and embedded snCMUTs. The peak-to-peak displacement of four-piston top plates is shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003ed. Variations in displacement were attributed to differences in the diameter of silicon nanocolumns fabricated via the top-down approach. The average volume displacement efficiency (\u003cem\u003eu\u003c/em\u003e\u003csub\u003e\u003cem\u003eavg\u003c/em\u003e\u003c/sub\u003e) was calculated as\u003csup\u003e\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e\u003c/sup\u003e\u003cdiv id=\"Equ1\" class=\"Equation\"\u003e\u003cdiv format=\"TEX\" class=\"mathdisplay\" id=\"FileID_Equ1\" name=\"EquationSource\"\u003e\n$$\\:{u}_{avg}=\\frac{1}{{A}_{tot}}{\\int\\:}_{{A}_{tot}}^{\\:}udA$$\u003c/div\u003e\u003cdiv class=\"EquationNumber\"\u003e1\u003c/div\u003e\u003c/div\u003e\u003c/p\u003e \u003cp\u003ewhere \u003cem\u003eu\u003c/em\u003e denotes the corresponding displacement, and \u003cem\u003eA\u003c/em\u003e\u003csub\u003e\u003cem\u003etot\u003c/em\u003e\u003c/sub\u003e represents the total plate area. The average volume displacement efficiency for the four cells was 6.86, 6.84, 3.92, and 5.34 nm/V\u003csub\u003ePP\u003c/sub\u003e, with a total average of 5.74 nm/V under 50 V DC bias and 1 V\u003csub\u003ePP\u003c/sub\u003e AC wave, which was five times higher than those reported previously. To compare the displacement profiles of the top plate during movement in conventional CMUTs and snCMUTs, the cross-sectional profile of snCMUT was used (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003ee). After normalising the cell dimensions and maximum displacement, the areas with 70% maximum displacement comprised 31% conventional CMUTs and 63% snCMUTs. Thus, snCMUTs generated top-plate movement over a broader area, resulting in higher average displacement efficiency and consequent greater transmission efficiency.\u003c/p\u003e \u003cp\u003eThe maximum output pressure and transmission efficiency of snCMUT were evaluated using a hydrophone in corn oil. The hydrophone with a pre-amplifier and oscilloscope monitored the pressure field of 3.5 mm away from the snCMUT elements with a DC bias voltage of 50 V and a 5 V\u003csub\u003ePP\u003c/sub\u003e AC wave at 4.65 MHz. Transient pressure amplitudes of 16.7, 27.5, and 37.5 kPa for 1, 4, and 10 channels, respectively, are shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003ef. The calculated maximum transient pressure at the surface was 1.51 MPa peak-to-peak, achieved with 5-cycle pulses at 4.65 MHz and 5 V\u003csub\u003ePP\u003c/sub\u003e. Approximately five cycles were necessary to transition from the asymmetric motion of the piston top plate, caused by the varying spring constants of individual silicon nanocolumns due to fabrication-related variances, to a symmetric motion influenced by the weight of the piston top plate (Supplementary Fig.\u0026nbsp;7). Herein, snCMUT exhibited a\u0026thinsp;\u0026minus;\u0026thinsp;3dB fractional bandwidth (FBW) of 31.61% at 4.65 MHz, which was lower than that of conventional CMUTs due to silicon nanocolumn fabrication variations and the weight of the piston top plate (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003eg). However, the \u0026minus;\u0026thinsp;6dB FBW of the frequency spectrum in pulse-echo response widened to 68.83% at 3.69 MHz due to damping by the lens after packaging snCMUT in a custom-made FPCB with a PDMS lens (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003eh). Therefore, variations in silicon nanocolumns and higher FBW could be achieved by using advanced lithography techniques.\u003c/p\u003e \u003cp\u003eComparative analyses of snCMUT with commercial lead zirconate titanate (PZT) probes, conventional CMUT, and enhanced CMUT (Supplementary Table\u0026nbsp;3) were conducted. Maximum pressure at the transducer surface was calculated to mitigate the impact of varying aperture sizes and focal lengths, accounting for acoustic attenuation and lens-induced gain. Commercial PZT transducers and conventional CMUT exhibited transmission efficiencies of 13.4 and 6.30 kPa/V, respectively\u003csup\u003e\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e,\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e\u003c/sup\u003e. Previous efforts improved CMUT transmission efficiency to 21.5 kPa/V using post-CMUTs\u003csup\u003e\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e\u003c/sup\u003e and 61.2 kPa/V using electrodepost CMUTs (EP-CMUT)\u003csup\u003e\u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e23\u003c/span\u003e\u003c/sup\u003e. Herein, snCMUT achieved a transmission efficiency of 220 kPa/V, a tenfold increase over post-CMUT and 3.6-fold over EP-CMUT. The parallel movement of the piston top-plate facilitated by silicon nanocolumns boosted the efficiency, enhancing volume displacement and transmission efficiencies with low voltage requirements. Thus, the proposed ultrasound transducers were proven to be highly suitable for wearable ultrasound technologies, offering high performance, low energy consumption, environmental adaptability, and disposability.\u003c/p\u003e \u003c/div\u003e\n\u003ch3\u003eComparative study on the ultrasound imaging performance of snCMUT on phantom\u003c/h3\u003e\n\u003cp\u003eConventional CMUTs directly connected to imaging systems generally suffer from degraded SNR due to large capacitances in flexible cables (hundreds of pF)\u003csup\u003e\u003cspan additionalcitationids=\"CR30\" citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR31\" class=\"CitationRef\"\u003e31\u003c/span\u003e\u003c/sup\u003e. To mitigate this issue, conventional CMUTs typically use application-specific integrated circuits (ASICs) placed close to the CMUT to improve SNR\u003csup\u003e\u003cspan additionalcitationids=\"CR30\" citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR31\" class=\"CitationRef\"\u003e31\u003c/span\u003e\u003c/sup\u003e. However, ASICs are expensive and present a significant challenge in developing wearable and disposable ultrasound patches.\u003c/p\u003e \u003cp\u003eIn contrast, snCMUT achieved sufficient SNR through its high transmission efficiency, eliminating the requirement for ASICs. Thus, system complexity and cost were reduced, along with lower power consumption, making snCMUT advantageous for use in wearable and disposable ultrasound patches. The fabricated disposable snCMUT patches included a custom-made FPCB directly connected to the Vantage 256 research ultrasound system, enabling simultaneous non-invasive monitoring of multiple body locations. The packaged patch was thin (933 \u0026micro;m, Supplementary Fig.\u0026nbsp;8) and suitable for attachment to the human body (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003ef). An in-house imaging code was developed to reconstruct the acquired images. To correct positional changes of each element in the wearable ultrasound patch in real-time, a technique from a previously published patent was used to detect relative positions by measuring capacitance changes between each singulated CMUT element (Supplementary Fig.\u0026nbsp;9). Image reconstruction was performed using a plane-wave compounding algorithm with 57 angles (Supplementary Fig.\u0026nbsp;10).\u003c/p\u003e \u003cp\u003eTo evaluate the wearable ultrasound imaging capabilities of the snCMUT patches, a multi-purpose ultrasound phantom (model ATS-539, CIRS) was used, combining monofilament line targets for distance measurements and tissue-mimicking target structures of varying sizes and contrasts (Supplementary Fig.\u0026nbsp;11a). For a comparative illustration of resolution between the disposable snCMUT patch and a conventional CMUT probe, images were captured in real-time up to a depth of 90 mm (Figs.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003ea and \u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eb). The conventional CMUT fabricated via the wafer bonding method was optimized for imaging by adjusting the DC bias voltage and AC voltage frequencies, operating at 70 V and 30.6 V\u003csub\u003ePP\u003c/sub\u003e at 6.25 MHz, respectively. The ultrasound images from the conventional CMUT showed axial-lateral resolution and anechoic targets up to a depth of 40 and 20 mm, respectively (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003ec, Supplementary Fig.\u0026nbsp;11b). The disposable snCMUT patch operated at a lower voltage compared to the conventional CMUT (DC bias voltage of 40 V and AC voltage of 8.9 V\u003csub\u003ePP\u003c/sub\u003e at 4.25 MHz). After packaging, the resonant frequency shifted from 4.65 to 4.25 MHz due to the damping effect of the PDMS lens covering the piston top plate. Ultrasound images from the snCMUT patches demonstrated the ability to resolve axial-lateral resolution targets at depths of approximately 75 mm, even at low voltages (Figs.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003ed and \u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003ee, Supplementary Fig.\u0026nbsp;11c). The anechoic target structure was distinguishable up to a depth of 30 mm.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eWhile analyzing ultrasound image intensity in the axial direction, the conventional CMUT showed the lowest background noise of approximately 18 mm and failed to distinguish the wire target beyond 40 mm. Conversely, the disposable snCMUT patch exhibited the lowest background noise at approximately 40 mm and produced distinguishable images up to 70 mm (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003ef). The disposable snCMUT patch achieved outstanding axial and lateral resolutions of 0.52 and 0.55 mm, respectively, in a 100 \u0026micro;m nylon monofilament phantom. Both the conventional CMUT probe and the disposable snCMUT patch demonstrated excellent axial and lateral resolutions below 1 mm compared to the commercial PZT-based probe. However, due to lower transmission efficiency, the conventional CMUT experienced a blurred image beyond 40 mm, while the snCMUT patch maintained sub-1 mm resolutions up to 70 mm (Figs.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eg and \u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eh). Furthermore, the snCMUT patch displayed higher ultrasound image contrast than the conventional CMUT probe, attributed to its superior SNR resulting from higher transmission efficiency (Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003ei).\u003c/p\u003e \u003cp\u003eThe superior performance of the disposable snCMUT patch was established by comparing it with previously reported wearable ultrasound probes (Supplementary Table\u0026nbsp;2). Recently published wearable ultrasound probes have achieved high frequencies and relatively high axial and lateral resolution in rigid forms, but their thickness exceeds 3 mm, limiting their applicability in wearable devices\u003csup\u003e\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e\u003c/sup\u003e. Stretchable ultrasound probes, while relatively thin at 1 mm, suffer from poor lateral resolution of 2 mm due to wide pitch and kerf\u003csup\u003e\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e,\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e\u003c/sup\u003e. Probes using PVDF are highly thin (0.1 mm) and flexible, but they require a high working voltage (95 VPP), trans-impedance amplifiers, and digitized amplification\u003csup\u003e\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e\u003c/sup\u003e. Contrarily, the disposable snCMUT patch is stretchable (excluding the FPCB) and fully packaged at less than 1 mm thickness. The disposable snCMUT patch achieved very high imaging quality, with axial and lateral resolutions of less than 0.55 mm, even at a very low working voltage of 8.9 V\u003csub\u003ePP,\u003c/sub\u003e owing to its high transmission efficiency and no requirements for ASICs to improve SNR.\u003c/p\u003e\n\u003ch3\u003eReal-time imaging and blood pressure waveform monitoring from carotid artery\u003c/h3\u003e\n\u003cp\u003eAfter evaluating and optimizing ultrasound images of the disposable snCMUT patches using a commercial multi-purpose phantom, the patches were applied to perform real-time monitoring of the carotid artery in the human neck (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003ea). Cardiovascular diseases, including heart conditions such as arrhythmia and vascular issues from cholesterol buildup, can disrupt blood flow in the carotid artery, leading to strokes\u003csup\u003e\u003cspan citationid=\"CR32\" class=\"CitationRef\"\u003e32\u003c/span\u003e,\u003cspan citationid=\"CR33\" class=\"CitationRef\"\u003e33\u003c/span\u003e\u003c/sup\u003e. Continuous monitoring of the artery is crucial for preventing such events. Wearable ultrasound patches offer continuous, non-invasive monitoring of arterial health, providing critical insights into cardiovascular status\u003csup\u003e\u003cspan citationid=\"CR33\" class=\"CitationRef\"\u003e33\u003c/span\u003e,\u003cspan citationid=\"CR34\" class=\"CitationRef\"\u003e34\u003c/span\u003e\u003c/sup\u003e. They improve patient compliance by eliminating the discomfort of traditional methods and enhance medication adherence through real-time feedback. Monitoring both carotid arteries is advantageous, as it helps detect asymmetrical arterial stiffness and localized issues such as atherosclerosis, offering a more comprehensive assessment of cardiovascular health\u003csup\u003e\u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e,\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e\u003c/sup\u003e. Furthermore, bilateral monitoring is used to identify significant blood pressure differences between the arteries, indicating severe conditions such as stenosis or aortic dissection\u003csup\u003e\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e\u003c/sup\u003e. This approach enhances patient safety and supports better treatment planning and postoperative care, particularly in high-risk patients and those undergoing carotid artery surgery.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eTwo disposable snCMUT patches were conformally placed in contact on both sides of the necks of 9 healthy volunteers for simultaneous monitoring of the left and right carotid arteries (Supplementary Fig.\u0026nbsp;12). To locate the carotid artery, the disposable snCMUT patch was moved while observing and acquiring B-mode images (Supplementary videos 1 and 2). For the safety of the subjects, the bias voltage was limited to 30 V. The disposable snCMUT patches were operated under 24.5 V\u003csub\u003ePP\u003c/sub\u003e of 4.25 MHz pulse. The vertical cross-sections of the common carotid artery (CCA) on the right (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003eb) and left (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003ec) sides of the neck were monitored via B-mode ultrasound images. The enhanced transmission efficiency provided by silicon nanocolumns allowed for relatively clear ultrasound images compared to previously reported wearable ultrasound imagers\u003csup\u003e\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e,\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e\u003c/sup\u003e, enabling the distinction of CCA and the internal jugular vein (JV) and sternocleidomastoid muscle (SCM). In the transverse ultrasound images of the carotid artery, the outer wall of the CCA was relatively clearly identified (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003ed), and pulsations were monitored in real-time. Despite operating at a very low voltage, the disposable snCMUT patches obtained more precise ultrasound images than previously reported wearable ultrasound imagers that required voltage amplification up to 200 V by boost converters with high power consumption. The low operating voltage was remarkable and required for wearable ultrasound patches.\u003c/p\u003e \u003cp\u003eThe blood pressure of the carotid artery acquired from the disposable snCMUT patches attached to the human neck through M-mode ultrasound imaging allowed continuous monitoring of the pathophysiological status (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003ee). The contraction and expansion of the carotid artery were measured through the positional changes of the anterior and posterior walls, tracking the blood pressure changes over time. The changes in the distance between carotid artery walls were converted into blood pressure pulse waves and compared with a clinical-grade tonometer. The disposable snCMUT patch exhibited over 96.5% agreement with the clinical-grade tonometer (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003ef, Supplementary Fig.\u0026nbsp;13), even preventing potential operator-dependent recording errors using handheld probes, such as vessel squeezing or misalignment (Supplementary Fig.\u0026nbsp;14). In healthy volunteers, there was little difference in blood pressure readings obtained simultaneously from both sides of the neck using the disposable snCMUT patches (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003eg), as healthy individuals typically do not exhibit carotid artery asymmetry. Continuous monitoring of the carotid arteries in patients using disposable snCMUT patches can help detect asymmetrical arterial stiffness and localized conditions such as atherosclerosis, offering a more comprehensive assessment of cardiovascular health. For this study, the human carotid artery was continuously monitored using low-cost, ultra-thin, silicon-based disposable snCMUT, obtaining detailed blood pressure pulse waveforms, including the maximal slope, systolic peak, dicrotic notch, and diastolic peak.\u003c/p\u003e"},{"header":"Conclusions","content":"\u003cp\u003eIn conclusion, developing disposable snCMUT patches marks a significant advancement in wearable ultrasound technology. The proposed patches offer a lead-free, flexible, and cost-effective solution for real-time medical imaging and monitoring. Using semiconductor fabrication on silicon wafers, snCMUTs present an eco-friendly alternative to traditional lead-based piezoelectric materials, significantly reducing manufacturing costs and making disposable probes a viable option. By incorporating silicon nanocolumns, the patches deliver high transmission efficiency and superior image resolution at low operational voltages, making them ideal for continuously monitoring critical parameters such as blood pressure and arterial health. Exceptional flexibility and thinness make snCMUT patches particularly suited for wearable applications, while their enhanced imaging performance and improved SNR have been validated in both phantom imaging and real-time carotid artery monitoring on both sides of the neck simultaneously. The innovation offers a promising approach to non-invasive cardiovascular health monitoring, providing valuable clinical insights with minimal discomfort and high diagnostic accuracy. Currently, the application of the low-cost, disposable snCMUT patches across various parts of the human body is under development to capture real-time three-dimensional ultrasound images, which is expected to enhance ultrasound imaging further and deliver crucial clinical insights.\u003c/p\u003e"},{"header":"Methods","content":"\u003cdiv id=\"Sec8\" class=\"Section2\"\u003e \u003ch2\u003eMaterials\u003c/h2\u003e \u003cp\u003eAll chemicals used during the fabrication of CMUT, including acetone, isopropyl alcohol (IPA), buffered oxide etchant (BOE) 6:1, hydrogen peroxide, and sulfuric acid, were purchased from Daejung Chemicals, Inc. (South Korea). Highly doped silicon wafers (p-type, 100) were purchased from WaferBiz (South Korea). On the other hand, highly doped SOI wafers (p-type, 100) were purchased from BMIT (South Korea). In conventional photolithography, two photoresist (PR) types were used: positive and negative PR. The positive PRs used were AZ GXR-601 (46 cP) and AZ 10-XT (520 cP) from Merk KGaA (Germany). The negative PRs, namely DNR-L-300-40 (120 cP) and DNR-L-300-D1 (21.8 cP), were purchased from Dongjin Semichem Co., Ltd. (South Korea). Aluminium etchant APAL-1 was purchased from Labotech (South Korea). Polydimethylsiloxane (PDMS; SYLGARD 160) for the acoustic lens was purchased from Dow Corning (USA).\u003c/p\u003e \u003c/div\u003e\n\u003ch3\u003eFabrication of silicon nanocolumns\u003c/h3\u003e\n\u003cp\u003eFour-inch bare silicon and SOI wafers were used to establish top-down fabrication of silicon nanocolumns at a single wafer level. The silicon and SOI wafers underwent a thermal treatment in an 1100 \u0026ordm;C furnace, resulting in the growth of a 200 nm silicon dioxide layer. Negative PR was applied to define silicon columns using conventional photolithography. Specifically, DNR-L300-D1 (21.8 cP) was spin-coated on the wafer. Following a baking step, UV light was exposed to the substrate using an EVG\u0026reg;6200 NT mask aligner (EV Group, Austria), and subsequent pre-exposure bake and development (AZ 300 MIF) processes were conducted. Furthermore, the silicon dioxide layer was etched using RIE via Plasmalab800Plus (Oxford Instruments, UK). DRIE was employed to define silicon columns at the micrometre scale using the Omega\u0026reg; LPX-DSi Etch system (SPTS Technologies Ltd., United Kingdom). A second round of RIE was applied to the micro-silicon columns to reduce their size through undercutting via horizontal etching, which smoothed the scallops from DRIE. Additionally, a systematic investigation was conducted on the anisotropy of the RIE process, and the etching conditions were fine-tuned to achieve precise fabrication of nano-sized silicon columns as springs.\u003c/p\u003e\n\u003ch3\u003eEvaluation of mechanical characteristics of silicon nanocolumns\u003c/h3\u003e\n\u003cp\u003eTo evaluate the top-down fabricated silicon nanocolumns, \u003cem\u003ein-situ\u003c/em\u003e nanomechanical characterizations were performed using a pico-indenter (Hysitron PI 85L PicoIndenter; Bruker, Corp., USA), which was integrated with the imaging capabilities of a scanning electron microscope (SEM) (Nova Nano SEM 450; FEI, Netherlands), allowing for simultaneous quantitative nanomechanical testing and imaging. A 10 \u0026micro;m diameter flat tip was employed to apply force to the nanocolumns, facilitating the assessment of their elastic deformation. To minimize buckling, the flat tip was positioned as perpendicular to the substrate as possible. For accurate characterization, the silicon nanocolumns were subjected to repeated compression by the pico-indenter across at least 10 individual samples, and the average displacement was recorded.\u003c/p\u003e \u003cdiv id=\"Sec11\" class=\"Section2\"\u003e \u003ch2\u003eDesign and fabrication of snCMUT array\u003c/h2\u003e \u003cp\u003eTo integrate silicon nanocolumns into CMUT as springs, FEA was conducted using the commercial tool COMSOL Multiphysics (COMSOL, Inc., USA). Nine silicon nanocolumns were arranged in a 3\u0026times;3 grid within a single cell of the CMUT, and each cell was designed as a square with dimensions of 120\u0026times;120 \u0026micro;m. A vacuum gap of 180 nm was designed for ultrasound generation. A single element in the CMUT array comprised 20 cells arranged in a 2-row and 10-column configuration. A piston structure (stiff block) was positioned above each cell to prevent the bending of the moving plate. The thickness of the moving plate was set at 2 \u0026micro;m, and the 64-element CMUT array was designed with a resonance frequency of 5 MHz for ultrasound imaging of the human carotid artery vessels.\u003c/p\u003e \u003cp\u003eThe fabrication involved wafer bonding of two SOI wafers, which had undergone DRIE and chemical mechanical polishing (CMP) to ensure precise dimensional control during fabrication. A SOI wafer with a device layer electrical resistivity of \u0026lt;\u0026thinsp;0.005 ohm-cm was selected to establish bottom and top plate electrodes.\u003c/p\u003e \u003cp\u003eFabrication details are provided in Supplementary Fig.\u0026nbsp;4. Initially, a silicon dioxide layer was grown and patterned on the SOI wafer using conventional photolithography techniques (EVG\u0026reg;6200 NT mask aligner, EV Group, Austria). Subsequent oxidation resulted in a vacuum gap height of 180 nm. The CMUT element and the lateral dimensions of the silicon nanocolumns were defined through DRIE using the Omega\u0026reg; LPX-DSi Etch system (SPTS Technologies Ltd., United Kingdom) after the silicon dioxide layer was etched using RIE via Plasmalab800Plus (Oxford Instruments, UK). The top-side SOI wafer was roughly aligned with the processed bottom SOI wafer, and wafer bonding was accomplished in a high-vacuum environment using the SB8 GEN2 system (Suss Microtec, Germany). The handle layer of the top SOI wafer was then grounded and polished to obtain 20 \u0026micro;m-thick piston top plates. A silicon dioxide mask was grown, accurately defining the piston structures and defining the top plate using DRIE. To establish electrical contact for the bottom and top plate electrodes, the layers including the buried oxide (BOX) layer, the device layer of the top SOI wafer, and the silicon dioxide layer in the bottom SOI wafer, were sequentially etched. The process culminated with the deposition and patterning of a 500 nm aluminum layer to create electrical contact pads. Each CMUT element was singulated via frontside and backside trenches formed by conventional photolithography and DRIE. The trenches were filled with PDMS to produce flexible and stretchable snCMUTs. Following fabrication, the processed SOI wafer was diced via blade dicing to segment the arrays of snCMUTs.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec12\" class=\"Section2\"\u003e \u003ch2\u003eCharacterization of snCMUT array\u003c/h2\u003e \u003cp\u003eFor evaluating the fabricated snCMUT array, SEM examinations were conducted using the Nova Nano SEM 450 (FEI, Netherlands). To carefully assess the shape of the silicon nanocolumns within the snCMUTs, focused ion beam (FIB) cutting was employed using NX 5000 (Hitachi, Japan). This process enabled precise vertical sectioning of the silicon nanocolumns, facilitating SEM observations to measure their shapes and dimensions. Additionally, the establishment of the vacuum gap and bonding of the moving plate were verified.\u003c/p\u003e \u003cp\u003eTo delve into the operational characteristics of the fabricated snCMUTs, an impedance analyser (4294A, Agilent Technologies Inc., USA) was used to confirm the phase and amplitude of the electrical input impedance, thereby assessing the electrical properties. A DC bias was applied to the snCMUT array via a bias T circuit and a high-voltage power supply, with a small-signal AC voltage of 100 mV superimposed (Supplementary Fig.\u0026nbsp;15). Calibration was meticulously conducted to invalidate potential parasitic influences. To analyze the mechanical movement of the moving plate of snCMUTs, an LDV integrated with a microscope (OFV-2570; Polytec GmbH, Germany) was employed (Supplementary Fig.\u0026nbsp;16). Measurement of the acoustic pressure generated by the snCMUT was conducted using a hydrophone (HGL-0200; Onda Co., USA) coupled with a pre-amplifier (Supplementary Fig.\u0026nbsp;17). The hydrophone signals were read out through a digitizing oscilloscope (Infiniium 54825A; Agilent Technologies Inc., USA). To simultaneously apply DC and AC voltages, a bias T circuit was used, with the DC voltage sourced from a high-voltage power supply (SRS PS310; Stanford Research System, USA) and the AC voltage applied through the AC function generator (Agilent 33500B; Agilent Technologies Inc., USA). The FBW of the transmitted ultrasound waves from the snCMUT array was calculated by acquiring the corresponding hydrophone signals. Conversely, the FBW of the pulse-echo signal from snCMUT, reflected from the metal bar in FC-40 immersed condition, was calculated by acquiring signals using an ultrasound pulser/receiver (5073PR, Olympus, Inc., USA).\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec13\" class=\"Section2\"\u003e \u003ch2\u003ePackaging and prototyping disposable snCMUT patch\u003c/h2\u003e \u003cp\u003eTo produce snCMUT for disposable patches, an in-house-designed FPCB was fabricated and employed. An adhesive was used to demonstrate the wearable CMUT's capability for ultrasound imaging to mount the fabricated snCMUT array. The FPCB was designed to include connectors for a commercial ultrasound imaging system (Vantage 256 research ultrasound system; Verasonics Inc., USA). Pre-tests were conducted using wire-bonding and anisotropic conductive film (ACF) for the electrical connection between snCMUT and FPCB. Since no significant differences were observed between the two methods, both methods were used for packaging. After electrical connections, 100 nm of parylene C was deposited via a parylene coating system (LAVIDA 110; FemtoScience, South Korea). The entire assembly was encapsulated with a PDMS lens for insulation and ultrasound focusing.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec14\" class=\"Section2\"\u003e \u003ch2\u003eAcoustic characterizations using commercial standard phantom\u003c/h2\u003e \u003cp\u003eIn this study, the conventional CMUT probe selected for comparison with snCMUT was fabricated using double oxidation and standard wafer bonding process\u003csup\u003e\u003cspan additionalcitationids=\"CR36\" citationid=\"CR35\" class=\"CitationRef\"\u003e35\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR37\" class=\"CitationRef\"\u003e37\u003c/span\u003e\u003c/sup\u003e, comprising a 192 channels linear array. Only 64 channels were operated for acoustic characterizations, the same as the number of channels in the snCMUT array. Imaging performance evaluation of both conventional CMUT probe and disposable snCMUT patch was conducted using standard ultrasound imaging phantom (ATS-539, CIRS) for dead zone group (2\u0026ndash;10 mm, with 1 mm spacing), vertical group (10\u0026ndash;180 mm, with 10 mm spacing), axial-lateral resolution array (40, 70, 110, and 160 mm, with 4, 3, 2, 1, 0.5, and 0.25 mm spacing) and anechoic target structures (10\u0026ndash;170 mm, with 20 mm spacing). Transducers were vertically placed on the surface of the phantom using ultrasound gel and operated using a commercial ultrasound imaging system (Vantage 256 system, Verasonics, Inc.) to scan over the phantom and reconstruct the imaging planes by employing a plane-wave compounding method, custom-programmed by MATLAB (MathWorks). Pulse-echo intensity, axial/lateral full width half maximum (FWHM) based on the point spread function (PSF) of the target, and ultrasound image contrast were analysed from the ultrasound images of the phantom targets acquired by both the conventional CMUT probe and disposable snCMUT patch.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec15\" class=\"Section2\"\u003e \u003ch2\u003eWearable B-mode imaging on the human body\u003c/h2\u003e \u003cp\u003eThe bio-interface excursions, carotid artery, and blood pressure waveforms were measured in healthy participants. All human tests were approved by the Seoul National University College of Medicine and Seoul National University Hospital, Institutional Review Board protocol (H-2007-145-1143). The participants provided their voluntary consent for ultrasonic measurements.\u003c/p\u003e \u003cp\u003eUsing the disposable snCMUT patches, real-time B-mode imaging on the human body was performed on nine healthy participants with relatively low and stable bias voltage for CMUT (30 V) provided by a commercial DC supply (PS310, Stanford Research Systems) and a transmitting voltage (24.5 V\u003csub\u003ePP\u003c/sub\u003e) provided by a commercial multi-channel controller, which was within the safe range of intensity defined by the Food and Drug Administration (\u0026lt;\u0026thinsp;190 W/cm\u003csup\u003e2\u003c/sup\u003e) to ensure the safety of the subject.\u003c/p\u003e \u003cp\u003eDisposable snCMUT patches were attached to the skin surface using commercial medical tape (3M Soft Cloth Tape with Liner 2762, 3M Company), manually aligned to the cross-sectional area of the CCA through real-time B-mode imaging. High-quality images of the CCA and adjacent JV and SCM were acquired using disposable snCMUT patches operated by the commercial multi-channel controller with a custom-programmed plane-wave compounding reconstruction algorithm.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec16\" class=\"Section2\"\u003e \u003ch2\u003eMeasurement and validation of blood pressure waveforms on carotid artery\u003c/h2\u003e \u003cp\u003eBased on the real-time B-mode imaging acquired by the disposable snCMUT patch, a single channel of the patch facing the top surface of the anterior wall of the CCA was manually selected for operation using M-mode imaging, specifically for measuring the CCA blood vessel diameter change over time acquired from pulse-echo signals from the anterior and posterior walls of the CCA. Using the wall tracking method, blood vessel diameter information was acquired and filtered to calculate the blood pressure waveforms of CCA.\u003c/p\u003e \u003cp\u003eTo validate the ability of the disposable snCMUT patches to measure blood pressure waveforms, the commercial pressure sensor-based blood pressure waveform analyser (DMP-1000 PLUS, DAEYOMEDI Co., Ltd., South Korea) was used to acquire blood pressure waveforms of CCA at the exact position of the same subject in close succession after the measurement using the disposable snCMUT patch. Systolic and diastolic blood pressure values from a commercial blood pressure cuff (JPN616T, OMRON Corporation, Japan) were additionally provided for calculating actual blood pressure for both disposable snCMUT patch and commercial pressure sensor.\u003c/p\u003e \u003c/div\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eData availability\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAll data supporting the findings and conclusions of this study are available within the paper and its Supplementary Information files. All other relevant data are available from the corresponding author upon request. Source data are provided in this paper.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgments\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (RS-2024-00341714), the internal program of the Korea Institute of Science and Technology (2E33141, 2E3312H), and the Korea Medical Device Development Fund grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health \u0026amp; Welfare, the Ministry of Food and Drug Safety) (RS-2023-00254676).\u003c/p\u003e\n\u003cp\u003eAll bio-experiments were conducted in accordance with the approval of the Institutional Review Board of the Seoul National University College of Medicine and Seoul National University Hospital.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthor contributions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eD.-H.K., SC, BT K.-Y., and BCL conceived and designed the research. HYK and BCL performed the simulations. D.-H.K., SC, HYK, SS, and BCL designed and performed all the fabrication and device measurements. D.-H.K., S.C., D.H.K, B.J., Y.S.L., E.-A.P., W.L., and B.C.L. performed all the clinical studies. D.-H.K., S.C., E.-A.P., W.L., H.K., M.I., J.-W.J., and B.C.L. analysed the data. J.-W.J. and BCL supervised the research and are project administrators. D.-H.K. and SC initially wrote the paper. All authors provided active and valuable discussions and feedback on the manuscript.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors declare no competing interests.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003eLa T, Le LH (2022) Flexible and Wearable Ultrasound Device for Medical Applications: A Review on Materials, Structural Designs, and Current Challenges. 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IEEE Trans Ultrason Ferroelectr Freq Control 60:1245\u0026ndash;1255\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"nature-portfolio","isNatureJournal":true,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"","title":"Nature Portfolio","twitterHandle":"","acdcEnabled":false,"dfaEnabled":false,"editorialSystem":"ejp","reportingPortfolio":"","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"wearable ultrasound imager, disposable patch, capacitive micromachined ultrasonic transducer, silicon nanocolumns, blood pressure monitoring","lastPublishedDoi":"10.21203/rs.3.rs-5309768/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-5309768/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eTraditional wearable ultrasound devices pose challenges concerning the rigidity and environmental impact of lead-based piezoelectric materials. This study proposes a novel silicon nanocolumn capacitive micromachined ultrasonic transducer (snCMUT) array for real-time wearable ultrasound imaging in disposable patches. Using a lead-free design, snCMUT incorporates silicon nanocolumns to address existing issues and achieves high transmission efficiency (220 kPa/V), flexibility, and low power consumption. The unique structure of snCMUT enhances displacement efficiency, enabling high-resolution imaging while maintaining a thin, flexible form factor (~\u0026thinsp;900 \u0026micro;m). Phantom imaging demonstrates its superior performance, with high axial and lateral resolutions (0.52 and 0.55 mm) and depth penetration (~\u0026thinsp;70 mm) at low voltage (8.9 V\u003csub\u003ePP\u003c/sub\u003e). Upon successful application to monitor both sides of the human carotid arteries, snCMUT offers clear ultrasound images and continuous blood pressure waveform monitoring. This proposed innovation presents significant potential for continuous medical imaging and cardiovascular health assessment, addressing environmental concerns and reducing manufacturing costs (\u0026lt;\u003cspan\u003e$\u003c/span\u003e20).\u003c/p\u003e","manuscriptTitle":"Silicon nanocolumn-based disposable and flexible ultrasound patches","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-01-15 12:09:40","doi":"10.21203/rs.3.rs-5309768/v1","editorialEvents":[],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"nature-communications","isNatureJournal":true,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"NCOMMS","sideBox":"Learn more about [Nature Communications](http://www.nature.com/ncomms/)","snPcode":"","submissionUrl":"https://mts-ncomms.nature.com/","title":"Nature Communications","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"ejp","reportingPortfolio":"Nature Communications","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"b102b360-59d6-4909-8afe-bb195e208ad7","owner":[],"postedDate":"January 15th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":40351011,"name":"Physical sciences/Nanoscience and technology/Nanoscale devices/NEMS"},{"id":40351012,"name":"Health sciences/Health care/Medical imaging/Ultrasonography"},{"id":40351013,"name":"Physical sciences/Engineering/Electrical and electronic engineering"},{"id":40351014,"name":"Physical sciences/Engineering/Biomedical engineering"}],"tags":[],"updatedAt":"2025-08-07T07:08:16+00:00","versionOfRecord":{"articleIdentity":"rs-5309768","link":"https://doi.org/10.1038/s41467-025-61903-x","journal":{"identity":"nature-communications","isVorOnly":false,"title":"Nature Communications"},"publishedOn":"2025-07-18 04:00:00","publishedOnDateReadable":"July 18th, 2025"},"versionCreatedAt":"2025-01-15 12:09:40","video":"","vorDoi":"10.1038/s41467-025-61903-x","vorDoiUrl":"https://doi.org/10.1038/s41467-025-61903-x","workflowStages":[]},"version":"v1","identity":"rs-5309768","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-5309768","identity":"rs-5309768","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}