Multi isotope analysis of seasonal variations and sources of sulfate in a typical thermal stratified reservoir

Multi isotope analysis of seasonal variations and sources of sulfate in a typical thermal stratified reservoir | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Multi isotope analysis of seasonal variations and sources of sulfate in a typical thermal stratified reservoir Li Li, Zhongfa Zhou, Jie Kong, Cui Wang, Yanbi Wang, Ru kai Xie, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5285977/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Seasonal stratification can alter the redox environment of lakes and reservoirs, and affect their water quality and biogeochemical cycles. Bacterial dissimilarity sulfate reduction (BSR) in anaerobic produces significant isotope fractionation. However, it is currently unclear whether the thermal stratification will lead to similar isotope fractionation characteristics in karst reservoirs. For this problem, samples were collected from Pingzhai Reservoir, a karst drinking water source reservoir in southwestern China, in April (spring), August (summer), November (autumn) of 2023, and January (winter) of 2024. By measuring the dissolved sulfate (SO2- 4) concentrations and multiple isotopes values (δ 34 S-SO2- 4, δ 18 O-SO2- 4and δ 18 O-H 2 O) in the inflow water, reservoir surface water, profile water, and outflow water, to trace the sources of SO2- 4 and analyze the biogeochemical processes in the reservoir. The results showed that SO2- 4 concentrations in the study area ranged from 11.76 to 132.52 mg/L, and the compositions of δ 34 S-SO2- 4 and δ 18 O-SO2- 4 values ranged from-7.66 to 7.52‰ and -5.94 to 5.05 ‰ ,respectively. The thermal stratification occurred between spring and autumn, but the BSR process mainly occurred in reservoir profiles from summer to winter. The enrichment coefficients of ɛ 34 S and ɛ 18 O were about 3.86‰ and 1.51‰, respectively, and no large isotope fractionation effect was found. The δ 18 O- SO2- 4 and δ 18 O-H 2 O values showed that sulfide oxidation processes occurred in all four seasons, and 37% to 95% of the oxygen atoms in the produced sulfates came from the surrounding water. Bayesian stable isotope mixing model showed that the sources of reservoir sulfate input were stable and that sulfide oxidation and soil sulfate dissolution were the main sources. The results of this study can help further understand the related effects of water chemistry and sulfur cycle processes in karst reservoirs. Earth and environmental sciences/Environmental sciences Earth and environmental sciences/Hydrology Earth and environmental sciences/Limnology sulfate thermal stratification multiple isotopes sulfate tracing karst reservoir Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5285977","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":377717904,"identity":"c217d889-e12e-4561-b886-e45ae286d57d","order_by":0,"name":"Li Li","email":"","orcid":"","institution":"Guizhou Normal University","correspondingAuthor":false,"prefix":"","firstName":"Li","middleName":"","lastName":"Li","suffix":""},{"id":377717905,"identity":"3c50fc14-a982-4b45-a720-82f1ec1868a9","order_by":1,"name":"Zhongfa 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of seasonal variations and sources of sulfate in a typical thermal stratified reservoir","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"sulfate, thermal stratification, multiple isotopes, sulfate tracing, karst reservoir","lastPublishedDoi":"10.21203/rs.3.rs-5285977/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-5285977/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eSeasonal stratification can alter the redox environment of lakes and reservoirs, and affect their water quality and biogeochemical cycles. Bacterial dissimilarity sulfate reduction (BSR) in anaerobic produces significant isotope fractionation. However, it is currently unclear whether the thermal stratification will lead to similar isotope fractionation characteristics in karst reservoirs. For this problem, samples were collected from Pingzhai Reservoir, a karst drinking water source reservoir in southwestern China, in April (spring), August (summer), November (autumn) of 2023, and January (winter) of 2024. By measuring the dissolved sulfate (SO2- 4) concentrations and multiple isotopes values (δ\u003csup\u003e34\u003c/sup\u003eS-SO2- 4, δ\u003csup\u003e18\u003c/sup\u003eO-SO2- 4and δ\u003csup\u003e18\u003c/sup\u003eO-H\u003csub\u003e2\u003c/sub\u003eO) in the inflow water, reservoir surface water, profile water, and outflow water, to trace the sources of SO2- 4 and analyze the biogeochemical processes in the reservoir. The results showed that SO2- 4 concentrations in the study area ranged from 11.76 to 132.52 mg/L, and the compositions of δ\u003csup\u003e34\u003c/sup\u003eS-SO2- 4 and δ\u003csup\u003e18\u003c/sup\u003eO-SO2- 4 values ranged from-7.66 to 7.52‰ and -5.94 to 5.05 ‰ ,respectively. The thermal stratification occurred between spring and autumn, but the BSR process mainly occurred in reservoir profiles from summer to winter. The enrichment coefficients of \u003cem\u003eɛ\u003c/em\u003e\u003csup\u003e34\u003c/sup\u003eS and \u003cem\u003eɛ\u003c/em\u003e\u003csup\u003e18\u003c/sup\u003eO were about 3.86‰ and 1.51‰, respectively, and no large isotope fractionation effect was found. The δ\u003csup\u003e18\u003c/sup\u003eO- SO2- 4 and δ\u003csup\u003e18\u003c/sup\u003eO-H\u003csub\u003e2\u003c/sub\u003eO values showed that sulfide oxidation processes occurred in all four seasons, and 37% to 95% of the oxygen atoms in the produced sulfates came from the surrounding water. Bayesian stable isotope mixing model showed that the sources of reservoir sulfate input were stable and that sulfide oxidation and soil sulfate dissolution were the main sources. The results of this study can help further understand the related effects of water chemistry and sulfur cycle processes in karst reservoirs.\u003c/p\u003e","manuscriptTitle":"Multi isotope analysis of seasonal variations and sources of sulfate in a typical thermal stratified reservoir","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-11-18 11:47:54","doi":"10.21203/rs.3.rs-5285977/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"809c17c8-bb4f-4fdb-a8f5-66a89e530ea8","owner":[],"postedDate":"November 18th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":40217417,"name":"Earth and environmental sciences/Environmental sciences"},{"id":40217418,"name":"Earth and environmental sciences/Hydrology"},{"id":40217419,"name":"Earth and environmental sciences/Limnology"}],"tags":[],"updatedAt":"2024-12-09T05:08:57+00:00","versionOfRecord":[],"versionCreatedAt":"2024-11-18 11:47:54","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-5285977","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-5285977","identity":"rs-5285977","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}