WITHDRAWN: Echium trygorrhizum Pomel root extract: A Novel Approach to Hyperglycemia management by inhibiting starch hydrolysis enzymes in vitro and in vivo

doi:10.21203/rs.3.rs-6477427/v1

WITHDRAWN: Echium trygorrhizum Pomel root extract: A Novel Approach to Hyperglycemia management by inhibiting starch hydrolysis enzymes in vitro and in vivo

2025 · doi:10.21203/rs.3.rs-6477427/v1

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Abstract

Abstract Echium trygorrhizum Pomel, is a wild species that grows in North Africa and belongs to the Boraginaceae family. In Algerian folk medicine, this species is used in the treatment of various conditions such as diabetes, jaundice, and angina. the present study we aimed to evaluate the therapeutic effects and the mechanism of action on type 2 diabetes management in rats and the antioxidant capacity and phytochemical analysis of the decocted aqueous extract of the roots obtained using a traditional method. To the best of our knowledge, no previous pharmacological studies have been performed in vivo on this plant. The Phytochemical composition of E. trygorrhizum Pomel roots aqueous extract (AQE) was analyzed using the HPLC-DAD technique. Four methods were used to investigate the antioxidant activity of our extract namely, DPPH free radical scavenging, ABTS radical cation scavenging activity, β- carotene / linoleic Acid Bleaching Activity, and CUPRAC. The inhibitory ability against α-glucosidase and α-amylase of AQE was assessed in vitro . Additionally, acute hypoglycemic tests, oral sucrose and glucose tolerance tests were performed on both healthy and alloxane-hyperglycemic rats. Investigation of the antioxidant activity demonstrated a significant oxidative reducing ability of our extract. Through HPLC-DAD analysis, myricetin and other compounds, namely fisetin, 2,4-dihydroxybenzoik acid, gallic acid, taxifolin, and naringenin, were identified, indicating high levels of phenolic compounds, including flavonoids, and tannins. In-vitro testing of AQE revealed considerable inhibitory ability against both α-glucosidase and α-amylase enzymes. Our results also indicated that AQE extract showed an antihyperglycemic effect in the oral sucrose tolerance test between 30 to 90 minutes after sucrose loading, whereas no decrease in blood glucose levels was detected for glucose tolerance and acute hypoglycemic tests. Our findings revealed that the AQE can effectively lower postprandial glycemia by inhibiting starch hydrolysis enzymes, namely, α-amylase and α-glucosidase, hence, the roots of our plant may serve as potential source for novel oral medication to treat hypoglycemia.

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WITHDRAWN: Echium trygorrhizum Pomel root extract: A Novel Approach to Hyperglycemia management by inhibiting starch hydrolysis enzymes in vitro and in vivo | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article WITHDRAWN: Echium trygorrhizum Pomel root extract: A Novel Approach to Hyperglycemia management by inhibiting starch hydrolysis enzymes in vitro and in vivo Allaoua Nouri, Iman RAMLI, Meriem HAMOUDI, Hakima Kebaili, Samira GHEDJATI, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6477427/v2 This work is licensed under a CC BY 4.0 License Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Editorial Note The full text of this preprint has been withdrawn by the authors in order to comply with an institutional policy on preprints. Therefore, the authors do not wish this work to be cited as a reference. Editorial notes are used to provide important context regarding the topic of a preprint or to alert readers to potential issues concerning that preprint or a downstream publication associated with it. For more information on editorial notes, see our Editorial Policies . Abstract The full text of this preprint has been withdrawn by the authors in order to comply with an institutional policy on preprints. Therefore, the authors do not wish this work to be cited as a reference. Full Text The authors have withdrawn this preprint from Research Square. Cite Share Download PDF Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. 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