Chemical Composition and antimicrobial activity of essential oils of two wild olive subspecies Olea europaea subsp. europaea var. Sylvestris and the endemic Olive Olea europaea subsp. lapperinie

Abstract Two wild olive subspecies fixed in this research: Olea europaea subsp. europaea var. sylvestris and Olea europaea subsp. laperrinei despite its ecological value, the chemical composition of subsp. laperrinei oil remains unknown, the samples were harvested from the different geographical area. Gas Chromatography-Mass Spectrometry(GC-MS) and Gas Chromatography-flame-ionization detection (GC-FID) analysis of subsp europaea var. sylvestris allowed the identification of 29 compounds oil with Nonanal (11.82%), theaspiranea A (9.81%), 3-hexen-1-ol,benzoate(9.31%) as a major constituents , while the subspecies of the Saharan region were resultedthe identification of 31 compounds where α-pinene (16%) , β-Ocimene (12.82%), dl-Limonene(8.20%) was the main components . The results of the disc diffusion method showed that the two volatile oils have efficient antibacterial activity but, subsp. laperrinei essential oil has a higher range of inhibition, in which P. aeruginosa and B. subtilis showed extreme sensitivity, while the K. pneumoniae bacterium shows great resistance to the two essential oils.