Monthly physical activity and storage time affect enzyme acetylcholinesterase activity in Plasma and Whole blood samples of healthy elderly subjects

Objective Enzyme Acetylcholinesterase is a potential marker for ageing, disease onset, progression and therapy. Physical activity and storage time affect the variable range, quality data prevalence and variability. The study aims to examine the effect of one and two months of storage time on protein concentration, acetylcholinesterase activity and inhibitor efficacy in subjects subjected to 3 months of physical activity. Method The value of AChE activity was measured spectrophotometrically by Elman et al. (1961). The obtained results were processed by the statistical program SPSS v 23.0. Results Group of 32 healthy subjects showed a significant difference (P<0.01) for protein concentration and acetylcholinesterase activity in Plasma, inhibitor Eserine efficacy in Plasma and Whole blood before and after physical activity. Based on Age, a significant difference is present for Height (P<0.01), Muscle mass (P<0.01) and protein concentration (P<0.05) in Whole blood after physical activity and storage time. A statistically significant difference between Gender exists for Age (P<0.01), Height (P<0.01), Weight (P<0.05), Muscle mass (P<0.05), protein concentration (P<0.05) in whole blood and acetylcholinesterase activity (P<0.05) in Plasma after physical activity. Conclusions Preliminary and descriptive data indicate no difference in acetylcholinesterase activity between Age groups. Physical activity and storage time influence the change of protein concentration, enzyme activity and inhibitor Eserine efficacy in healthy elderly subjects. Future studies should compare disease and healthy participants.