Genome-wide identification and expression analysis of related DNA methylation genes has revealed the potential role in the response to salt stress in Chrysanthemum

Genome-wide identification and expression analysis of related DNA methylation genes has revealed the potential role in the response to salt stress in Chrysanthemum | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 9 August 2025 V1 Latest version Share on Genome-wide identification and expression analysis of related DNA methylation genes has revealed the potential role in the response to salt stress in Chrysanthemum Authors : Zhongya Shi , Yanchao Han , Wenya Liu , Xingyuan Wang , Wenchao Tan , Wenqian Zhao , Zhongai Li , Penghui Ai , and Zicheng Wang [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.175471769.91074756/v1 162 views 96 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Cytosine-5 DNA methyltransferases (C5-MTases) and DNA demethylases (dMTases) are essential for plant growth, development, and responses to biotic/abiotic stresses. Despite chrysanthemum being a major ornamental crop, its C5-MTase and dMTase genes remain largely uncharacterized. Here, we identified 69 C5-MTase and 24 dMTase genes in four chrysanthemum species. Phylogenetic analysis classified these genes into 4 (C5-MTase) and 3 (dMTase) subfamilies, respectively. Among these four chrysanthemum species, the genes are distributed across 7–19 chromosomes, with segmental duplication as the primary driver of their family expansion. Expression profiling revealed distinct spatiotemporal expression patterns of all C5-MTase / dMTase genes in different tissues, as well as their differential responses to abiotic stresses (salt and drought). Functional characterization showed that ClMET1a -overexpressing Arabidopsis exhibited a significantly lower germination rate than the wild type (WT) after 24 hours of 150 mM salt treatment. RNA-seq analysis identified the nitrate transporter gene AtNRT2.6 , whose promoter methylation level was significantly elevated in overexpressing lines. This suggests that ClMET1a may regulate AtNRT2.6 promoter methylation, thereby modulating Arabidopsis germination under salt stress. Overall, this study provides critical insights into the roles of C5-MTase and dMTase gene families in chrysanthemum development and stress adaptation. Supplementary Material File (main manuscript.doc) Download 47.52 MB Information & Authors Information Version history V1 Version 1 09 August 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords development growth Authors Affiliations Zhongya Shi Henan University College of Life Science View all articles by this author Yanchao Han Henan University College of Life Science View all articles by this author Wenya Liu Henan University College of Life Science View all articles by this author Xingyuan Wang Henan University College of Life Science View all articles by this author Wenchao Tan Henan University College of Life Science View all articles by this author Wenqian Zhao Henan University College of Life Science View all articles by this author Zhongai Li Henan University College of Life Science View all articles by this author Penghui Ai Henan University College of Life Science View all articles by this author Zicheng Wang [email protected] Henan University College of Life Science View all articles by this author Metrics & Citations Metrics Article Usage 162 views 96 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Zhongya Shi, Yanchao Han, Wenya Liu, et al. Genome-wide identification and expression analysis of related DNA methylation genes has revealed the potential role in the response to salt stress in Chrysanthemum. Authorea . 09 August 2025. DOI: https://doi.org/10.22541/au.175471769.91074756/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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