Isolation and characterization of a novel H3N6 from a Harbor seal (Phoca vitulina) in the North Pacific

Isolation and characterization of a novel H3N6 from a Harbor seal (Phoca vitulina) in the North Pacific | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 17 January 2025 V1 Latest version Share on Isolation and characterization of a novel H3N6 from a Harbor seal (Phoca vitulina) in the North Pacific Authors : Wendy Puryear 0000-0002-1795-3399 [email protected] , Rodrigo Canas-Arranz , Kaitlin Sawatzki , Nichola Hill , Alexa Simulynas , Natalie Rouse , Courtney Pace , … Show All … , Kathy Burek , Adriana Van De Guchte , Zain Khalil , Ana Gonzalez-Reiche , Rebecca L Pearl , Susana Guerra , Harm Van Bakel , Randy A Albrecht , Adolfo García-Sastre , and Jonathan Runstadler Show Fewer Authors Info & Affiliations https://doi.org/10.22541/au.173714759.92201883/v1 318 views 174 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Background Influenza A Viruses (IAV) are emerging pathogens of public health concern. Wild birds and mammals are important reservoirs that contribute to the evolving genetic diversity of influenza viruses and represent an important transmission interface that may facilitate mammalian adaptation of IAV. Detection of IAV within wild mammalian species provides important insights to potential mechanisms of interspecies transmission and adaptation. Methods A novel H3N6 IAV was recovered from a stranded harbor seal (Phoca vitulina) in Alaska. The virus was sequenced, isolated, and tested for replication in mouse and ferret models. Results Analysis of the full genome sequence suggested the novel seal derived H3N6 IAV was derived from a reassortment of H3N8 and H4N6 circulating in dabbling ducks in the Pacific flyway. Assessment of replication and virulence phenotypes in mouse and ferret models of influenza provided evidence of replication in mammals despite minimal disease. Conclusions. We report the first identification of H3N6 within marine mammals with capacity for replication within mammalian hosts despite the absence of previously described adaptations. Field surveillance studies combined with risk assessment pipelines that incorporate phylogenetic analysis and in vitro and in vivo characterization are essential for understanding mammalian adaptation. Supplementary Material File (seal influenza h3n6 complete preprint.pdf) Download 1.20 MB Information & Authors Information Version history V1 Version 1 17 January 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords h3n6 harbor seal influenza pacific reassortment risk assessment Authors Affiliations Wendy Puryear 0000-0002-1795-3399 [email protected] Cummings School of Veterinary Medicine at Tufts University View all articles by this author Rodrigo Canas-Arranz Department of Microbiology, Icahn School of Medicine at Mount Sinai Icahn School of Medicine at Mount Sinai, Global Health and Emerging Pathogens Institute View all articles by this author Kaitlin Sawatzki Cummings School of Veterinary Medicine at Tufts University View all articles by this author Nichola Hill University of Massachusetts Boston View all articles by this author Alexa Simulynas Cummings School of Veterinary Medicine at Tufts University View all articles by this author Natalie Rouse Alaska Veterinary Pathology Service, Eagle River View all articles by this author Courtney Pace Alaska Sealife Center View all articles by this author Kathy Burek Alaska Veterinary Pathology Service, Eagle River View all articles by this author Adriana Van De Guchte Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai View all articles by this author Zain Khalil Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai View all articles by this author Ana Gonzalez-Reiche Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai View all articles by this author Rebecca L Pearl Department of Microbiology, Icahn School of Medicine at Mount Sinai Icahn School of Medicine at Mount Sinai, Global Health and Emerging Pathogens Institute View all articles by this author Susana Guerra Department of Microbiology, Icahn School of Medicine at Mount Sinai Icahn School of Medicine at Mount Sinai, Global Health and Emerging Pathogens Institute Department of Preventive Medicine, Public Health and Microbiology, Universidad Autónoma de Madrid View all articles by this author Harm Van Bakel Department of Microbiology, Icahn School of Medicine at Mount Sinai Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai Icahn Genomics Institute, Icahn School of Medicine at Mount Sinai View all articles by this author Randy A Albrecht Department of Microbiology, Icahn School of Medicine at Mount Sinai Icahn School of Medicine at Mount Sinai, Global Health and Emerging Pathogens Institute View all articles by this author Adolfo García-Sastre Department of Microbiology, Icahn School of Medicine at Mount Sinai Icahn School of Medicine at Mount Sinai, Global Health and Emerging Pathogens Institute Department of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai View all articles by this author Jonathan Runstadler Cummings School of Veterinary Medicine at Tufts University View all articles by this author Funding Information National Institute of Allergy and Infectious Diseases 75N93021C00014 Metrics & Citations Metrics Article Usage 318 views 174 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Wendy Puryear, Rodrigo Canas-Arranz, Kaitlin Sawatzki, et al. Isolation and characterization of a novel H3N6 from a Harbor seal (Phoca vitulina) in the North Pacific. Authorea . 17 January 2025. DOI: https://doi.org/10.22541/au.173714759.92201883/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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