Study on the value of isolation and extraction of pathological positive cells to improve the detection rate:control trial

ABSTRACT OBJECTIVE The density of pathological positive cells was calculated by flow cytometry, and the target cells were isolated and extracted to improve the detection rate. DESIGN a case-control study. SETTING Hematology Department of Hospital PARTICIPANTS Selected from MDS 33 cases, 17 cases of malignant tumors, 29 cases of lupus erythematosus, 4 cases of plasmodium falciparum disease, a total of 76 cases as positive blood specimens. MAIN OUTCOME MEASURES Design: Research group (group I) : Flow cytometry was used to analyze and calculate the density of various cell types, and then cell separation solution was used to accurately separate and extract target cells of the same density by adjusting the gradient of two different densities, that is, to collect pathologically positive blood cells of the same density. A control group (group II) was established: the whole blood samples of the same patient were compared and analyzed by direct push-film observation. RESULTS As shown in Figure 2AB-3AB-4AB-5AB, A large number of positive abnormal cells were extracted from group I, which improved the accuracy and specificity of diagnosis. In the control group (group II), only a few positive abnormal cells were found, which was very easy to misdiagnose and miss diagnosis. Data are analyzed from Table 3. According to the statistical results of group I, the positive detection rates were MDS=76%, malignant tumor cells =53%, lupus erythematosus cells =62%, Plasmodium falciparum =75%. In the control group (group II), the positive detection rate was MDS=27%, malignant tumor cells =11%, lupus erythematosus cells =33%, Plasmodium falciparum =25%. Chi-square test was used to compare the mean value of independent samples between groups, and the difference was statistically significant (P<0.05). CONCLUSIONS The density of pathologically positive blood cells can be measured by flow cytometry according to the specimens that need to be reviewed by microscopy as indicated by blood routine, and the target cells extracted can be accurately separated, that is, the same density of pathologically positive blood cells can be collected. Combined with the gold standard method of artificial microscopy, the detection rate was significantly improved, and the occurrence of misdiagnosis and missed diagnosis was reduced. For the difference in cell density of various forms in different types of specimens, it only needs to be distinguished from normal cell density, and abnormal pathological positive cells can be accurately extracted, which can help diagnose the positive detection rate of diseases, which is of great significance and value