Site-specific Halogenation of Peptides and Proteins using engineered Halogenase Enzymes
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Abstract
We demonstrate novel in vitro halogenation of peptides by halogenase enzymes, and identify the (G/S)GW motif (HaloTryp Tag) as a preferred substrate. We further derive PyrH halogenase mutants showing improved halogenation of the HaloTryp Tag, both as a free peptide and when genetically fused to model proteins.
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- europepmc
- last seen: 2026-05-19T01:45:01.086888+00:00