Expression of cytochrome P450 and esterase genes involved in permethrin resistance in larvae and adults of Culex quinquefasciatus

Mosquitoes are the important vectors of several arthropod diseases, which remain a prioritized domain for epidemiology research. Mosquito vector control strategies have classically relied on adult management using chemical insecticides like synthetic pyrethroids. The indiscriminate and non-judicial practice of pesticides has caused tolerance of mosquito population to insecticides of choice. The evolvement of tolerance relies on the expression of few orinterplay of many gene products, belonging to cytochrome P450 and carboxylesterase and glutathione superfamily. The present investigation involves the expression pattern of six cytochrome P450 and five esterase candidate genes CYP6M2, CYP6AA7, CYP6Z2, CYP9J34, CYP6BTQ6B7, CYP6AE14 and α-Esterase, Esterase B1, Neuroactin, KM234968, KM234962 in larval and adults Culex quinquefasciatus permethrin resistance strain. Results found expression of the genes CYP6AA7 by 10 folds in larval PerRes and an adult, the expression was only 4.5 folds (P  0.05; F-0.2496). Esterase gene was 9.0 folds over-expressed in larva and 2.5 folds in adults (P < 0.001; F-27.75). Based on the expression profile the present study suggests that the cytochrome P450 CYP6A and esterase gene family may contribute metabolic resistance to permethrin in larval and adult Cx. quinquefasciatus strain.